AIM: To investigate the effect of Guishen Capsule(Radix Angelicae Sinesis,Radix et Rhizoma Salivae Miltiorrhizae,etc) on mastoplasia and T-lymphocyte subsets of spleen in rats. METHODS: The model of mastoplasia was induced by benzoate estradiol intraperitoneal injection in SD female rats.The model rats were treated with low,middle and high dosages of Guishen Capsule,respectively.All rats were killed after 30 days,the tissue of second pair of mammary glands were observed.At the same time,the spleens were taken out for preparing single cells suspension.The ratio of CD~+_3,CD~+_4,CD~+_8,CD~+_4/CD~+_8 of splenic T-lymphocytes were determined by flow cytometry(FCM). RESULTS: Compared with normal group,the growth of weight was slow in model group,and its breast was swollen,and proliferation was obvious in mammary gland tissue,the ratio of CD~+_3,CD~+_4,CD~+_4/CD~+_8 reduced significantly in model group. Guishen Capsule could inhibit the breast swelling and mammary gland hyperplasia.It also could increase ratio CD~+_3,CD~+_4,CD~+_4/CD~+_8 of splenic T-lymphocytes remarkably. CONCLUSION: Guishen Capsule can inhibit mammary gland hyperplasia.It also has the function of immunologic enhancement and cellular immune regulation.

BACKGROUND: Calcium phosphate cement (CPC) can strengthen the immediate and early fixation of screws by improving the quality of bone and the surface of screws. OBJECTIVE: To evaluate the biomechanical efficacy of CPC in augmentation of anterior cervical unicortical screw fixation. DESIGN: A study of controlled observation. SETTING: Department of Orthopaedics of First Xiangya Hospital of Xiangya Medical College of Central South University. MATERIALS: Experiments were performed at the Room of Electromechanical Tensile Property of College of Material Science and Engineering of Central South University from September 2003 to January 2004. Anterior cervical unicortical screw (pure titanium) was produced by Zhangjiagang Xinda Medical Equipment Co., Ltd. Injectable calcium phosphate cement was produced by Shanghai Ruibang Biomaterial Co., Ltd. Axial pull-out sleeve was produced by Machine Manufacture Center of Central South University. METHODS: ①Sixteen cervical vertebrae (C3-6) of four fresh young man cadavers with fine results in bone mineral density (BMD) test and sixteen cervical vertebrae (C3-6) of 4 fresh old man cadavers with poor results in BMD test were selected. Specimens were provided by Department of Anatomy of Xiangya Medical College of Central South University. Mortal remains of the deceased were donated according to the will of the dead. Informed consents were obtained from their family members. Twelve vertebrae were selected in each group for three tests. Six vertebrae were selected in pull-out test, and six vertebrae in cyclic bending test and shearing test. Screw holes, which were 8 mm apart from the midline and 5°inclined centrally, were made on both sides in the front part of the vertebra. Cortical bone was not penetrated. ②A screw was randomly inserted into one side hole, which was considered to be a control group. Pull-out test was performed on material testing machine at 5 mm/min. The screw hole was filled with CPC (0.10-0.15 mL) to repair the screw hole, and then the screw was implanted, which was considered to be a restoration group. The screw hole was injected with CPC, and then the screw was implanted, which was considered to be a augmentation group. 24 hours later, pull-out test was conducted again at 37 ℃. ③The screw was randomly implanted into one side hole (as a control group). The screw hole was injected with CPC, and then screw was implanted (as an augmentation group). Both groups received cyclic bending test and shearing test. MAIN OUTCOME MEASURES: ①The maximal axial pull-out strength of screws, ②displacement of screws after cycle bending test, ③the maximal shear strength after cycle bending test. RESULTS: ①The axial pull-out test: In normal vertebrae, axial pull-out strength was (313±64)N, (376±88)N and (446±121)N in the control group, the restoration group and the augmentation group respectively. In osteoporotic vertebrae, axial pull-out strength was (106±47)N, (154±67) N and (191±80)N in the control group, the restoration group and the augmentation group respectively. The axial pull-out strength in the restoration group and augmentation group was significantly bigger than in the control group (P < 0.05). ②The cyclic bending test: The displacement of the unicortical screw in the augmentation group was much smaller than in the control group (P < 0.05). ③ In normal vertebrae, the maximal shear strength was (301±79)N and (395±105)N in the control group and the augmentation group respectively. In the osteoporotic vertebrae, the maximal shear strength was (87±39)N and (149±63)N in the control group and the augmentation group respectively. The maximal shear strength was significantly bigger in the augmentation group than in the control group (P < 0.05).CONCLUSION:CPC can enhance the axial pull-out strength and shearing ability of the screw. The augmentation is more evident in osteoporosis patients.

Objective To search different weeks′ gestation atrioventricular size and cardiac function change in Hb Bart′s fetal edema syndrome. Methods Color Doppler echocardiography was adopted to study 67 cases with Hb Bart′s fetal edema syndrome in different weeks′ gestation.Atrial and ventricular diameter and cardiac function were measured, and compared with those of normal fetuses in same weeks′ gestation.Results In the cases with Hb Bart′s fetal edema syndrome,the heart was enlarged from 25 weeks′ gestation,right atrial enlargement came earliest, along with gestation weeks′ followed with right ventricle,left atrium and left ventricle aggravated enlargement.From 29 weeks′ gestation left and right cardiac diastolic function was involved with complicated pericardial,pleural,peritoneal effusion the damage was clearer.During 33 weeks′ gestation,left and right heart systolic function was involved.From 25 weeks′ gestation,fetal foramen ovale began to enlarge,and the enlarged foramen was difficult to be differentiated from secondary atrial septal defect.Conclusions Echocardiography is sensitive to Hb Bart′s fetal edema syndrome,and provides reliable basis for early clinical intervention.

The effects of magnolol (Mag) on hyperglycemia and hyperlipemia, hepatic oxidative stress and cytochrome P4502E1 (CYP2E1) activity of diabetic rats induced by high-fat diet (HFD) and streptozotocin (STZ) were studied. After oral administration of Mag (25, 50 and 100 mg x kg(-1) x d(-1)) for continuous 10 weeks, the blood glucose and lipids (TC, TG and LDL-C) levels, as well as the hepatic CYP2E1 activity and MDA content of diabetic rats, decreased significantly (P < 0.05 or P < 0.01), whereas the oral glucose tolerance and hepatic antioxidant enzymatic activities (CAT and GSH-Px) of diabetic rats, increased significantly (P < 0.05 or P < 0.01). The results indicated that Mag was effective against the hepatic oxidative damage, hyperglycemia and hyperlipemia of diabetic rats induced by HFD and STZ, and the inhibition of Mag on hepatic CYP2E1 activity could be an important mechanism of Mag against hepatic insulin resistance and oxidative damage.
Animals ; Biphenyl Compounds ; isolation & purification ; pharmacology ; Blood Glucose ; metabolism ; Cholesterol ; blood ; Cholesterol, LDL ; blood ; Cytochrome P-450 CYP2E1 ; metabolism ; Diabetes Mellitus, Experimental ; blood ; drug therapy ; metabolism ; Diet, High-Fat ; Glucose Tolerance Test ; Hypoglycemic Agents ; isolation & purification ; pharmacology ; Lignans ; isolation & purification ; pharmacology ; Liver ; metabolism ; Magnolia ; chemistry ; Male ; Oxidative Stress ; drug effects ; Plants, Medicinal ; chemistry ; Protective Agents ; pharmacology ; Rats ; Rats, Wistar ; Streptozocin ; Triglycerides ; blood

Objective To investigate the Th1/Th2 polarization of T lymphocytes in human peripheral blood stimulated by CpG-ODN2216,and the secretion of cytokines in supernatant of cultured PBMCs after stimulation of CpG-ODN2216.Methods Human PBMCs were isolated from blood of donors.The PBMCs were incubated with CpG-ODN2216 for 24 hours.Th1/Th2 subsets in the cultured PBMCs were examined by flow cytometry,and IFN-?,IL-2,IL-4 and IL-10 in the supernatant were assayed by ELISA.Results The percentage of Th1 and Tc1 increased significantly after stimulation of CpG-ODN2216 compared with control group (P0.05).Conclusion The Th1 cells and Tc1 cells in T lymphocytes of peripheral blood could be polarizated by CpG-ODN 2216.IFN-? secretion in PBMCs could be induced by CpG-ODN2216.

Objective The forensic application of Lefort aqua regia digestion method detecting diatom in rabbits’s organs. Methods Using the Lefort aqua regia digestion-membrane ifltrition-SEM observation method and the traditional acid digestion-centrifugation-light microscopy observation method to detect diatom of drowning or postmortem immersion rabbits’s organs(lung,liver,kidney), and make statistical analysis of diatom number, consuming time, digestive capability and ifne structure of diatoms. Results The diatom number of Lefort aqua regia digestion method was superior to conventional acid digestion method, with the difference being statistically signiifcant (P<0.05), as well as consuming time, but have undifferentiated of drowning grounp(P>0.05), and have a better observation effect. Conclusion The Lefort aqua regia digestion method for diatom extraction was helpful for diatom identiifcation., and useful in drowning test by conventional and economicly.

Objective To observe the influence of the theory of planned behavior (TPB) in teaching patients with cervical spondylosis about their disease.Methods A hundred cervical spondylosis patients were assigned according to their admission order into an experimental or a control group.The control group received conventional health education and health education materials, including oral health education.With the intervention group,the TPB was used to design their nursing, and they received behavioral intervention addressing attitude, subjective norms and behavior control.Both groups were assessed for the degree of pain using a six-point pain score, health behavior, and recurrence, before the experiment and after 6 months.Results After six months both groups had significantly improved pain scores, but the average improvement in the intervention group was significantly greater.Their health behavior was also significantly better except for the prevention of pharyngeal infection and keeping warm.Conclusions The TPB can significantly relieve the pain and improve the health behavior of spondylosis patients, and reduce the recurrence rate.

Acute Disease ; Administration, Inhalation ; Adolescent ; Asthma ; drug therapy ; Budesonide ; administration & dosage ; Child ; Child, Preschool ; Female ; Humans ; Male ; Oxygen ; Terbutaline ; administration & dosage

The effect of amygdalin joint hydroxysafflor yellow A (HSYA) on the endplate chondrocytes derived from intervertebral discs of rats induced by IL-1beta and the possible mechanism were studied and explored. Chondrocytes were obtained from endplate of one-month SD rat intervertebral discs and cultured primary endplate chondrocytes. After identification, they were divided into normal group, induced group, amygdalin group, HSYA group and combined group. CCK-8 kit was adopted to detect the proliferation of the endplate chondrocytes. FCM was measured to detect the apoptosis. Real-time PCR method was adopted to observe the mRNA expression of Aggrecan, Col 2 alpha1, Col 10 alpha1, MMP-13 and the inflammatory cytokines IL-1beta. The protein expression of Col II, Col X was tested through immunofluorescence. Compared with the normal group, the proliferation of the endplate chondrocytes decreased while the apoptosis increased (P < 0.05). With down regulation of the mRNA expressions of Aggrecan, Col 2 alpha1 and up regulation of the mRNA expressions of Col 10 alpha1, MMP-13, IL-1beta (P < 0.05), the protein expression of Col II decreased while the protein expression of Col X increased. Compared with the induced group, amygdalin group, HSYA group, the combined group could inhibit the apoptosis and promote the proliferation (P < 0.05). They could increase the mRNA expressions of Aggrecan and Col 2 alpha1 while decrease the mRNA expressions of Col 10 alpha1, MMP-13 and IL-1beta (P < 0.05). They could also enhance the protein expression of Col II while reduce the protein expression of Col X. The effect of the combined group was significantly better than that of amygdalin and HSYA. Amygdalin joint HSYA could inhibit the degeneration of the endplate chondrocytes derived from intervertebral discs of rats induced by IL-1beta and better than the single use of amygdalin or HSYA.
Amygdalin ; pharmacology ; Animals ; Apoptosis ; Cells, Cultured ; Chalcone ; analogs & derivatives ; pharmacology ; Chondrocytes ; drug effects ; Collagen ; metabolism ; Drug Synergism ; Interleukin-1beta ; Intervertebral Disc ; cytology ; Quinones ; pharmacology ; Rats