1.A study on the lipids of Ascaris suum ova.
Soon Hyung LEE ; Chul Yong SONG ; Keun Bai LEE ; Hi Sung LEE
The Korean Journal of Parasitology 1977;15(1):43-50
The present study was undertaken to observe the quality and quantity of lipid constituents by the developing Ascaris suum eggs. The collected eggs from the uterus of A. suum were classified into 3 groups, i.e., single cell stage, morula stage and embryonated eggs, and were subjected to analyse their lipid fractions. To obtain the morula stage eggs, 10 to 11 incubation days at 30 degree C were needed and for the embryonated eggs, 30 to 31 days were lasted. At the time of experiment, their indices of development by Hoffman were 285(morula stage) and 42 (embryonated stage) respectively. Lipid extraction was done by the methods of Folch et a1. (1957) and Kenny (1952), and then the extracted lipid fractions from the above 3 groups of eggs were separated by thin layer chromatography. Those fractions were also subjected to perform the quantitative analyses of fatty acids, glycerides, cholesterol and phospholipids. The results obtained were summarized as follows. Total amount of fatty acid was decreased from 12.9 mg per gram of eggs (single cell stage) to 6.6 mg/gm (embryonated eggs), whereas the proportion of free fatty acid to total fatty acid was constantly increased from 77.5 percent to 89.4 percent during the period of egg development. Total amount of glycerides was also increased from 33.0 mg/gm of single cell stage to 55.9 mg/gm of the embryonated eggs. The most abundant glyceride among 3 glycerides discovered from A. suum eggs was triglyceride, and the least was monoglyceride. The amount of free cholesterol was much larger than that of ester form in general, and it reached maximum in the eggs of morula stage (4.6 mg/gm). The increase of total cholesterol was monitored during the development of A. suum eggs from 3.3 mg/gm to 5.4 mg/gm. The following 8 phospholipids were detected in the embryonated eggs, i.e., lysophosphatidyl choline, phosphatidyl inositol, sphingomyelin, phosphatidyl choline, phosphatidyl glycerol, phosphatidyl serine, phosphatidyl ethanolamine and unknown phospholipid. But in the single cell stage eggs, 4 kinds out of the above 8 phopholipids were not observed, and in the morula stage eggs, 2 kinds were absent among the 8 phospholipids.
parasitology-helminth-nematoda
;
Ascaris suum
;
ovum
;
lipid
;
fatty acid
;
glycerides
;
triglyceride
;
monoglyceride
;
cholesterol
;
lysophosphatidyl choline
;
phosphatidyl inositol
;
sphingomyelin
;
phosphatidyl choline
;
phosphatidyl glycerol
;
phosphatidyl serine
;
phosphatidyl ethanolamine
;
phopholipids
;
biochemistry
2.A Case of Mayer-Rokitansky-Kster-Hayser Syndrom.
Kyoung Suk LEE ; Jeong Bae KANG ; Hong Bai KIM ; Keun Yong LEE ; Sung Won KANG
Korean Journal of Obstetrics and Gynecology 1997;40(8):1751-1755
A perplexing developmental error is 46,XX congenital absence of vagina ( Mayer-Rokitan-sky-Kster-Hauser syndrome, Mullerian dysgenesis, vaginal aplasia ). The Mayer-Rokitansky-Kster-Hauser syndrome refers to the climical entity consisting of primary amenorrhea associated with congenital absence of the vagina, 46,XX karyototype, a rudimentary uterus or complete absense, normal overian function and normal ovulation, normal female breast development, body proprotion and body hair, frequent association of renal, skeletal and other cpngenital anomalies. This syndrome results from agenesis of both Mllerian ducts or from a failure of the Mllerian ducts to estavlish a proper communication with that part of the vagina from the urogenital sinus. A case of mayer-Rokitansky-Kster-Hauser syndrom was reviewed briefly.
Amenorrhea
;
Breast
;
Female
;
Hair
;
Humans
;
Ovulation
;
Uterus
;
Vagina
3.Clinical analysis of surgical geriatric patients over 65 years of age.
Jae Chull UM ; Dong Whan CHOI ; Yong Bai LEE ; Sung Chul KIM ; Kwang Tae KIM
Journal of the Korean Surgical Society 1993;44(3):439-448
No abstract available.
Humans
4.Usefulness of insulin - like growth factors in predicting reduced bone mass in natural postmenopausal women.
Ki Bum AHN ; Jung Gu KIM ; Kwang Bum BAI ; Jin Yong LEE
Korean Journal of Obstetrics and Gynecology 2000;43(10):1813-1821
No abstract available.
Female
;
Humans
;
Insulin*
;
Intercellular Signaling Peptides and Proteins*
5.A Case of Malignant Mixed Mullerian Tumor of Uterus.
Sang Wook BAI ; See Yong KIM ; Yoon Ho LEE ; Dong Hoon WHANG
Korean Journal of Gynecologic Oncology and Colposcopy 1993;4(4):118-122
A Malignant mixed miillerian tumor of uterus is rare and highly malignant containing epithelial and mesenchymal components. A case of mixed germ cell tumor was exprienced and presented with a brief review of concerned literature.
Neoplasms, Germ Cell and Embryonal
;
Uterus*
6.Hypobaric Spinal Anesthesia in a Patient with Transplanted Heart: A case report.
Sun Joon BAI ; Yong Taek NAM ; Haeng Chul LEE ; Min Woo KOO
Korean Journal of Anesthesiology 1998;35(5):999-1002
Heart transplantation is an accepted procedure for treatment of end-staged cardiac failure. A return to near-normal quality on life can be expected in many patients with a nonrejecting cardiac allograft, and many of these patients will return to the operating room for noncardiac surgical procedures. Anesthesiologists should be alert to recognizing problems caused by the presence of infection in immunosuppressed patients, modes of presentation of rejection phenomena and how transplanted organs, notably significantly denervated ones, may behave and respond under the pathophysiologic circumstance that arise during surgery, resuscitation and intensive care. The use of regional techniques require adequate preloading to avoid exaggerated hypotension and aseptic technique to avoid infection. Hypobaric spinal anesthesia has some benefit. It does not depress cardiovascular and respiratory system and keep adequate venous return by trendelenberg position. We report herein a case of successfully undergone total hip replacement in a patient who had previously undergone orthotopic heart transplantation under hypobaric spinal anesthesia.
Allografts
;
Anesthesia, Spinal*
;
Arthroplasty, Replacement, Hip
;
Heart Failure
;
Heart Transplantation
;
Heart*
;
Humans
;
Hypotension
;
Critical Care
;
Operating Rooms
;
Respiratory System
;
Resuscitation
7.Acute gastric volvulus due to diaphragmatic hernia: a report of one case.
Jae Chull UM ; Dong Whan CHOI ; Yong Bai LEE ; Sung Chul KIM
Journal of the Korean Surgical Society 1993;44(6):911-915
No abstract available.
Hernia, Diaphragmatic*
;
Stomach Volvulus*
8.A clinical analysis of abdominal trauma.
Keong Chul JANG ; Yong Bai LEE ; Sung Chul KIM ; Kwang Tae KIM
Journal of the Korean Surgical Society 1992;42(5):679-691
No abstract available.
9.Rapid Diagnosis of Isoniazid Resistance by Detection of Mutations in katG and inhA of Mycobacterium tuberculosis from Korea.
Sang Jae KIM ; Seok Yong KIM ; Ji Youn LEE ; Sang Ryeol RYU ; Gil Han BAI
Journal of the Korean Society for Microbiology 1997;32(5):569-576
29 isoniazid (INH) resistant isolated strains and INH sensitive reference strain (H37Rv) of Mycobacterium tuberculosis were analysed by polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) and NciI restriction mapping for the detection of mutations in katG gene and inhA gene. The katG gene was divided into 3 parts (Akat, Bkat, Ckat; each part is about 800 bp) and amplified, inhA gene was amplified as a whole. Each of the amplified 800 bp DNA was digested into small fragments of less than 400 bp with restriction enzymes for the direct PCR-SSCP analysis. Firstly, 10 strains were analysed. All the 10 isolates showed clearly distinct SSCP patterns in Bkat from that of the reference strain, but only two isolates showed distinct SSCP patterns in Akat, and no isolated strain showed any distinct SSCP patterns in Ckat. 10 isolates also showed distinct SSCP patterns in inhA. NciI restriction mapping of Bkat showed mutation in codon 463 in 7 strains among 10 isolated strains. With these results an early detection strategy for the INH resistant M. tuberculosis was applied to the rest of 19 isolated INH resistant strains. Firstly, isolates were screened by Ncsl mapping in Bkat, and 13 strains showed mutations in codon 463. Secondly, the rest of 6 INH resistant isolates were analysed by PCR-SSCP with restriction enzyme digestion (PCR-SSCP-RE) in Bkat, and all the strains showed distinct SSCP patterns from that of the INH sensitive reference strain. This proved our strategy as effective and economic and time saving method in early detection of INH resistant M. tuberculosis.
Codon
;
Diagnosis*
;
Digestion
;
DNA
;
Isoniazid*
;
Korea*
;
Mycobacterium tuberculosis*
;
Mycobacterium*
;
Polymorphism, Single-Stranded Conformational
;
Restriction Mapping
;
Tuberculosis
10.A clinical study on the incompetent internal os of the cervix.
Young In LEE ; Dong Soo CHA ; Jang Yeon KWON ; In Bai CHUNG ; Yong Jin LEE ; Sang Won HAN ; dae Hyun KIM
Korean Journal of Obstetrics and Gynecology 1993;36(7):1475-1481
No abstract available.
Cervix Uteri*
;
Female