Objective To compare the effect and safety of venlafaxine sustained-release tablet combined with Shuganjieyu capsule and venlafaxine sustained-release tablet used singlely in the treatment of moderate to severe poststroke depression.Methods 70 patients suffered from moderate to severe post-stroke depression were randomly divided into two groups,35 cases in each group.The patients in the control group were only treated with venlafaxine sustained-release tablet,and the patients in the study group were treated by venlafaxine sustained-release tablet combined with Shuganjieyu capsule.The treatment lasted 6 weeks.The therapeutic effect and safety were evaluated by the positive and negative syndrome scale of HAMD (17 items),and treatment emergent symptom scale of TESS.Results After treatment for 6 weeks,the total effective rate of the study group was 88.57％,which was significantly higher than 65.71％ of the control group (x2 =5.19,P ＜ 0.05).The HAM D score of the study group w.as (16.42 ± 6.07) points,and the HAMD score of the control group was(10.54 ± 5.23) points after 6 weeks.The difference was statistically significant between the two groups (t =4.34,P ＜ 0.01).The difference of TESS score between two groups was not significant(t =0.18,P ＞ 0.05).Conclusion Venlafaxine sustained-release tablet combined with Shuganjieyu capsule is effective and safe in the treatment of moderate to severe post-stroke depression.

Objective To investigate the occurence of glomus tumor and investigate its diagnosis and treatment. Methods 13 cases of glomus tumors were analyzed retrospectively. There were 9 females and 4 males. All 13 cases were surgically treated under megaloscpe. Results All the cases were testified as glomua tumors by pathology. There was no recurrence during the follow up of 6 months to 1 year. All the patients were symptom free. Conclusion Glomus tumor has specific features on clinical presentation. Mierosurgieal excision under megaloscpe is fill elective method.

Objective To discuss the application value of diffusion weighted imaging(DWI)in the diagnosis and treatment of synovial sarcoma.Methods 48 patients with synovial sarcoma were selected.All patients were given conventional magnetic resonance imaging (MRI)and DWI detection examination,DWI diffusion sensitive factor examination was 500 s/was(b).All the patients'imaging results and disease detection were analyzed.ROC curve was used to analyze the value of DWI,MRI examination in the diagnosis of synovial sarcoma.Results DWI examination detection of synovial sarcoma was much higher than that of MRI,the difference was statistically significant(χ2 =5.62, P <0.05).ROC curve analysis showed that MRI examination in the diagnosis of synovial sarcoma specificity was 72.58%,sensitivity was 79.12%,the accuracy was 86.82%,DWI examination in the diagnosis of specificity was 76.81%,sensitivity was 85.79%,the accuracy was 95.18%.The sensitivity,specificity and accuracy of DWI were significantly higher than conventional MRI,the differences were significant(χ2 =5.72,5.68,5.12,all P <0.05). Conclusion DWI can effectively improve the detection rate of synovial sarcoma,and has higher diagnostic sensitivity, specificity and accuracy,it's conducive to the physician for treatment in a timely manner,and worthy of further clinical promotion.

Angina pectoris is a common belonging to the diseases of "Chest Bi-syndrome", "precordial pain" or "syncopal precordialgia" in traditional Chinese medicine. Clinically Shengmai injection, Acanthopanex injection, Breviscapine injection, Fleabane injection, Safflower injection, Astragalus injection, Shuxuetong injection, Xin-ding injection and Panax notoginsenoside injection were commonly used to treat the disease with good clinical curative effect.

Objective To explore clinical and neuroimagine features, as well as the management of rare ectopic pituitary adenoma in clivus. Methods We reported two cases of ectopic pituitary adenoma verified by histology in the clivus region, reviewed the relevant literature briefly, summarized the clinical and neuroimaging features, along with the management of ectopic pituitary adenoma in clivus. Results Ectopic pituitary adenoma in clivus caused symptoms of endocrinopathy and/or neurological dysfunction. Imaging studies showed an occupying lesion in the clivus. Pituitary adenoma was diagnosed in one patient preoperatively, the other was diagnosed as chordoma. The tumors were microsurgically removed through the trans-sphenoidal route. Tumor was radically removed in one patient, and during one-year follow-up, there was no recurrence. The other was partially resected, and suffered postoperative cerebrospinal fluid rhinorrhea and intracranial infection, follow-up of this patient lost finally. Conclusions The patients with ectopic pituitary adenoma in clivus have endocrinopathy or/and neurological dysfunction, and MRI plays a valuable role in the diagnosis. Although extremly rare, ectopic pituitary tumour needs to be considered in case of an occupying lesion in the clivus. Radical resection of tumor can cure the patient.

Objective: To explore the influence of granulocyte-colony stimulating factor (G-CSF) combined with adrenocortical steroid and/or immunosuppressants on immune system of donor mice during hematopoietic stem cell mobilization. Methods: The donor C57BL/6 mice were divided into 4 groups according to the mobilization strategies, namely, the G-CSF group (250 μg/kg for 4 days), G-CSF (250 μg/kg for 4 days) + methylprednisolone group (100 mg/ kg on day 3), G-CSF (250 μg/kg for 4 days) + methylprednisolone group (100 mg/kg on day 3) + cyclosporine A (100 mg/kg on day 3), and PBS control group. The post-mobilization T lymphocytes of C57BL/6 mice and the splenocytes of BALB/c mice were subjected to mixed lymphocyte reactions (MLR). The levels of IFN-γ, IL-4, and TGF-β 1 were determined in the supernatants of donor mice splenocytes by ELISA method and the mRNA levels of IFN-γ and IL-4 were determined by RT-PCR. Results: The results showed that the MLR-inhibitory activities in the 3 G-CSF mobilized groups were 42 %, 21 %, and 39 %, respectively; all lower than that in PBS control group (68%). IFN-γ levels in the 3 G-CSF mobilized groups were lower than that in PBS control group, but he levels of IL-4 and TGF-β 1 were significantly higher than those in PBS group(P<0.01), with no significant difference found between the 3 G-CSF mobilized groups. RT-PCR analysis found that the expression of IFN-y mRNA was obviously decreased and the expression of IL-4 was obviously increased (P<0.01). Conclusion: G-CSF combined with adrenocortical steroid and/or immunosuppressants can decrease the allogenic reaction of T lymphocytes and change the types of secreted cytokines in donor mice during hematopoietic stem cell mobilization, which may induce immune resistance in the recipients.

Humans ; Meniere Disease

A satisfaction survey carried out by Beijing Chao-Yang Hospital during the Beijing 2008 Olympic Games, found differences of patient satisfaction between locals and overseas patients in consulting environment, outpatient procedure, quality of service, quality of diagnosis and treatment. The patient satisfaction of overseas patients was lower than locals. Especially noteworthy is the low satisfaction of the former for the hospital coloring, privacy in the sector, registration, vocal communication, patient condition statement, nurse attitude and service quality. Real-time improvement in these aspects of medical services has harvested obvious progress. Results of the satisfaction survey provided valuable reference materials for large-scale activities, and ongoing improvement of medical services in the long run.

BACKGROUND: It has been reported that magnesium sulfate(Mg2SO4)treatment has a satisfactory effect on brain ischemia-reperfusion injury, but its effect on spinal cord ischemic injury remains unclear.OBJECTIVE: To evaluate the effects of intravenous administration of Mg2SO4 on spinal cord ischemia-reperfusion injury and further probe into its mechanism.DESIGN: Randomized controlled repeatedly measuring design based on the experimental animals.SETTING: Central research laboratory of a university hospital.MATERIALS: The experiment was conducted in the Central Research Laboratory, Medical College of Xi' an Jiaotong University from April 2003 to June 2004. Twenty-seven New Zealand white rabbits with body mass of 1.9to 2. 5 kg were included. The rabbits were randomly divided into Mg2SO4group, normal saline group and sham-operation group with 9 rabbits in each group.METHODS: The lower segment of the kidney under abdominal aorta was occluded for 30 minutes and 48-hour reperfusion was performed to establish ischemia-reperfusion model of lumbosacral segment of spinal cord. Mg2SO4group (Group A, n = 9) received Mg2SO4 at the dose of 0. 25 mL/kg per hour throughout this procedure; the same volume of saline solution was used in normal saline group(Group B, n=9) . Animals of sham-operation group (Group C, n = 9) were anesthetized and received laparotomy without aortic occlusion. The somatosensory evoked potential(SEP) was detected before ischemia, 30 minutes after ischemia, and 1, 2, 8, 16 and 24 hours after reperfusion. Motor function score was assessed in Mg2SO4 and saline groups 24 and 48 hours after reperfusion. After reperfusion for 48 hours, the animals were killed and histopathological test was performed on the spinal cord.MAIN OUTCOM MEASURES: Motor function score, SEP monitoring and spinal histopathological test.RESULTS: The latency of SEP(Nt) was markedly longer 30 minutes after ischemia in Mg2SO4 group. It was obviously recovered during the first two hours after reperfusion compared with during ischemia, but was obviously prolonged after that. Waveform disappeared 30 minutes after ischemia in normal saline group. SEP amplitudes and latencies in sham-operation group did not change remarkably during the procedures and all the animals recovered without neurological deficits. At each reperfusion time point, the recovery of SEP(N1) latency was better in Group A than that in Group B( P ＜ 0.05). The average motor function score at 24 hours and 48 hours after reperfusion was significantly higher in Group A[ (3.7 ±0.5) and(3.4 ±0.7) points] than that in GroupB [(3.0±0.7) and (2.6±0.9) points](P ＜0.05). The normal nerve cell counting of spinal cord 48 hours after reperfusion in Mg2SO4 group(23. 4 ± 3. 4) was significantly higher than that in saline group (12.3 ±3.2)(P ＜ 0.01).CONCLUSION: Intravenous Mg2SO4 administration may reduce spinal cord injury and preserve neurological function in transient spinal cord ischemia in rabbits.

BACKGROUND:Previous research has demonstrated human bone marrow mesenchymal stem cells(HMSCs)differentiate into hepatocyte-like cells;however,biological characteristics and differentiation mechanism remain unclear,and differentiation system remains immature.OBJECTIVE:To investigate the feasibility of hepatocyte growth factor(HGF)and epidermal growth factor(EGF)to induce the differentiation of HMSCs into hepatocyte-like cells.METHODS:HMSC5 were obtained from patients with esophageal cancer and were separated by density gradient centrifugation combined with attachment method.The phenotypes of MSCs were identified by flow cytometry.The third-passage HMSCs were divided into four groups:HGF(adding 20 μg/L HGF),EGF(adding 20 μg/L EGF),HGF+EGF,and blank control groups.Morphology was observed using inverted microscope.At days 7 and 14 after induction,α-fetoprotein and albumin mRNA expressions were detected using RT-PCR assay.RESULTS AND CONCLUSION:The HMSCs did not express hematopoietic cell CD34 and CD35,but strongly expressed β1-integrin CD29 and matrix receptor CD44.HMSCs changed from long fusiform shape to polygon or similar round shape in the HGF,EGF,and HGF+EGF groups.At days 7 and 14 after induction,α-fetoprotein and albumin mRNA expressions were positive.However,polygon cells were not observed in the blank control group,and α-fetoprotein and albumin mRNA expressions were negative.This suggested that HGF,EGF,and HGF+EGF could induce the differentiation of HMSCs into hepatocyte-like cells;however,their differentiation ability still needs to be further semi-quantitatively analyzed using immunohistochemical staining.