This article analysis the present situation of less than 35 years of Sun Yat-sen university youth scientific and technological personnel,they declare、undertake to support basic research based science fundation related cases,discusses the science foundation for their assistance and training problems,put forward youth scientific talents to suit the science funds development requirements to promote their development proposals

OBJECTIVE:To compare the effects of2different treatment methods on the assaying of triptolide alcohol in tripterygium wilfordii cut crude drug.METHODS:After concentration,tripterygium wilfordii cut crude drug water decoction underwent column chromatographic separation after adsorption with neutral alumina(Method1)and neutral alumina column chromatographic separation after ethanol precipitation and extraction by chloroform(Method2),respectively.Triptolide alcohol was determined by HPLC and the assaying result underwent pairing and t tests.RESULTS:There was significant difference between the2methods with the content determined by Method1significantly higher than that by Method2(P

Alteration of H-and M-isozymfs of Lactic Dehydrogenase(LDH) were observed in the various tissues after exposing the rats to 50ppm and 250ppm of sulfur dioxide. These isozymes of the respective tissue were separated by Diethlaminoethyl(DEAE)-cellulose from tile tissue homogenates of brain, lung and muscle, presenting the activities by rate of reduction of nicot inamids-adenine-dinucleotide (NAD+). Pure LDH and the coenzyme NAD (NAD) were directly treated with sulfur dioxide in vitro order to find out the direct to sulfur dioxide on LDH and NAD+ and the results were as follow. 1. In the normal tissues, the H-isozyme activity was dominant in the brain and heart, and tole M-isozyme in the muscle. 2. In the lung tissue of normal rats, there was no different between tole activity of H-and M-type of LDH. 3 When rats inhale sulfur dioxide gas in concentration of 50ppm and 230ppm, it appeared that the H-type tend to be suppressed in aerobic tissues and the M-type in anaerobic tissues. 4. In the lung tissue exposed to sulfur dioxide, both the LDH activities were suppressed. 5. It seems that LDH and the coenzyme (NAD ) are not directly affected by exposing in sulfur dioxide gas.
Animals ; Brain ; Heart ; Isoenzymes ; Lung ; NAD ; Rats ; Sulfur Dioxide* ; Sulfur*

Autophagy is an intracellular degradation system that delivers cytoplasmic constituents to the lysosome and it's involved in the cycle of matter and energy. Meanwhile autophagic cell death is a form of cell death called type-Ⅱ programmed cell death. In recent years, studies have shown that autophagy is involved in pathophysiologies of nervous system disease especially neurodegenerative diseases, ischemia-hypoxia, traumatic brain injury ,seizures.

Objective To observe the clinical efficacy on primary biliary cirrhosis(PBC) treated by two methods. Methods 50 cases with PBC were divided into the combination therapy group(A group:n =25) and simple western medicine treatment group(B group:n = 25) ;The curative effect and indicators of liver function were observed before and after treatment in patients with PBC. Results A total effective in group A 19 cases(76.0%) were higher than in B group 15 cases (60.0%) (x~2 = 3.87, P ＜ 0.05) ;The clinical symptoms and signs in A、B group after treatment were better than before treatment(x~2 = 3. 87 ～ 6. 89,P ＜ 0.05 ～ 0.01) ;The clinical symptoms and signs in A group after treatment were better than B group(x~2 = 3.88 ～ 4. 12. ,all P ＜0.05) ;The indicators of biochemical in A、B group after treatment were lower than before treatment(t = 2. 214 ～ 2. 517, P ＜ 0.05); The indicators of biochemical of ALP、GGT、ALT in A group decreased significantly compared with B group(t = 2. 284 ～ 2. 418, P ＜0.05). Conclusion Integrative Chinese and western medicine achieves good clinical efficacy in patients with PBC.

Objective To evaluate the curative effects of Hybrid external fixator in the treatment of severely comminuted and open fractures of the distal or proximal tibia. Methods From March 2004 to December 2008,we treated 27 patients with severely comminuted and open fractures of the distal or proximal tibia with the Hybrid frame purchased from Orthofix Co.in combination with limited internal fixation if necessary.The internal fixation was done with screws in 6 cases,with absorbable interference serews in 4 and with wire in 3.There were 16 males and 11 females,with an average age of 56.2(13 to 80)years.Accordins to Gustilo-Anderson classification,there were 10 cages of typeⅡ,12 cases of type Ⅲ A and 5 cases ofⅢB.The injuries were mostly caused by traffic accident and fall.Postoperative ankle and/or knee funetions were evaluated by Johner-Wrubs system.Results The patients were followed up for 7 to 58 months(average,27 months).Averagely the patients obtained bone union at 6.7 months and fixator removal at 6 months after operation.The postoperative JohnerWruhs score was excellent in 17 cases.good in 8 and fair in 2.The total exceHent to good rate was 92.6%,without wound infection or complications of nerve and vessel injury.There were only 5 cages of local superficial pin tract infection which responded to oral antibiotics and disinfectants. Conclusion In treatment of severely comminuted and open fractures of the distal or proximal tibia,Hybrid frame fixation may lead to limited invasion,reliable fixation,low rate of complications,and early recovery of joint function.

Description of a multi-perspective view of harmonious doctor-patient relationship holds that medical social workers as a key tie for building such a relationship.It proposed the medical social work strategies for such workers in the face of patients and their families,hospital staff,and hospital management.

Humans ; Laser Therapy ; Male ; Prostatic Hyperplasia ; surgery

Adolescent ; Humans ; Scoliosis ; etiology ; genetics

Humans ; Kyphosis ; complications ; surgery ; Osteotomy ; methods ; Scoliosis ; complications ; surgery ; Treatment Outcome