This article analysis the present situation of less than 35 years of Sun Yat-sen university youth scientific and technological personnel,they declare、undertake to support basic research based science fundation related cases,discusses the science foundation for their assistance and training problems,put forward youth scientific talents to suit the science funds development requirements to promote their development proposals

OBJECTIVE:To compare the effects of2different treatment methods on the assaying of triptolide alcohol in tripterygium wilfordii cut crude drug.METHODS:After concentration,tripterygium wilfordii cut crude drug water decoction underwent column chromatographic separation after adsorption with neutral alumina(Method1)and neutral alumina column chromatographic separation after ethanol precipitation and extraction by chloroform(Method2),respectively.Triptolide alcohol was determined by HPLC and the assaying result underwent pairing and t tests.RESULTS:There was significant difference between the2methods with the content determined by Method1significantly higher than that by Method2(P

Alteration of H-and M-isozymfs of Lactic Dehydrogenase(LDH) were observed in the various tissues after exposing the rats to 50ppm and 250ppm of sulfur dioxide. These isozymes of the respective tissue were separated by Diethlaminoethyl(DEAE)-cellulose from tile tissue homogenates of brain, lung and muscle, presenting the activities by rate of reduction of nicot inamids-adenine-dinucleotide (NAD+). Pure LDH and the coenzyme NAD (NAD) were directly treated with sulfur dioxide in vitro order to find out the direct to sulfur dioxide on LDH and NAD+ and the results were as follow. 1. In the normal tissues, the H-isozyme activity was dominant in the brain and heart, and tole M-isozyme in the muscle. 2. In the lung tissue of normal rats, there was no different between tole activity of H-and M-type of LDH. 3 When rats inhale sulfur dioxide gas in concentration of 50ppm and 230ppm, it appeared that the H-type tend to be suppressed in aerobic tissues and the M-type in anaerobic tissues. 4. In the lung tissue exposed to sulfur dioxide, both the LDH activities were suppressed. 5. It seems that LDH and the coenzyme (NAD ) are not directly affected by exposing in sulfur dioxide gas.
Animals ; Brain ; Heart ; Isoenzymes ; Lung ; NAD ; Rats ; Sulfur Dioxide* ; Sulfur*

An experiment on seminal vesiculitis in rats induced by repeated injections of homologous and heterologous seminal vesicle tissue suspension with Freund's complete adjuvant was accomplished to produce an immune disease in the seminal vesicle for the purpose to find the cause of same type of lesion in human beings were divided into 7 In this study 65 healthy adult white rats which weighed from 150 to 250 gm. were divided into 7 groups are follows: Group 1. Six rats, control, injected with normal saline Group 2. Six rats, injected with adjuvant Group 3. Seven rats, injected with seminal vesicle tissue suspension Group 4. Six rats, injected with rat seminal vesicle tissue suspension and adjuvant with prednisolone Group 5. Seven rats, injected with rabbit seminal vesicle tissue suspension Group 6. Seven rats, injected with rabbit seminal vesicle tissue suspension with adjuvant. Rats in these 6 groups were injected weekly for 8 weeks and sacrificed on the sixty fourth day. Group 7. Twenty-six rate, rat seminal vesicle tissue suspension with adjuvant. Rats in this group were subdivided into four groups Group A. Five rats, injected 4 times and sacrificed on 64th day. Group B. Seven rats, injected 6 times and sacrificed on 64th day. Group C. Seven rats, injected 8 times and sacrificed on 64th day. Group D. Seven rats. injected 8 times and sacrificed on 92nd day. Seminal vesicles from all the rats sacrificed were histopathologically studied and results were obtained as follows. Significant histopathologic alterations suck as glandular atrophy, decreased secretoryactivity, interstitial fibrosis, edema. congestion and inflammatory cellinfiltration suggesting of noninfectious chronic seminal vesiculitis were obtained in most rats of Group 7. Severity of inflammatory reaction seemed to be accentuated according to the increase of frequency of the date of sacrifice not concerned. Those allergic reaction were appeared to be inhibited by administration of prednisolone.
Adult ; Animals ; Atrophy ; Edema ; Estrogens, Conjugated (USP) ; Fibrosis ; Humans ; Hypersensitivity ; Immune System Diseases ; Prednisolone ; Rats ; Seminal Vesicles*

Although alpha2-adrenoceptor agonists are widely used as postoperative sedatives in adults, the postoperative effects in pediatric patients with secondary pulmonary arterial hypertension (PAH) due to congenital heart disease are not well known. We experienced a case of successful ventilator weaning with continuous intravenous administration of dexmedetomidine (DEX) after surgical correction in a 46-month-old child with congenital ventricular septal defect (VSD) with severe PAH. She underwent VSD closure on cardiopulmonary bypass (CPB). After successful weaning from the CPB, hemodynamics and oxygenation were stabilized on DEX and nitroglycerin in the intensive care unit. The patient was successfully weaned from the ventilator 46 hours after surgery. The transthoracic echocardiogram two weeks after surgery showed a closed VSD with no residual shunt and trivial tricuspid regurgitation (Vmax = 2.5 m/sec) without PAH.
Administration, Intravenous ; Adult ; Cardiopulmonary Bypass ; Child* ; Child, Preschool ; Dexmedetomidine* ; Heart Defects, Congenital ; Heart Septal Defects, Ventricular* ; Hemodynamics ; Humans ; Hypertension* ; Hypnotics and Sedatives ; Intensive Care Units ; Nitroglycerin ; Oxygen ; Tricuspid Valve Insufficiency ; Ventilator Weaning ; Ventilators, Mechanical ; Weaning

Autophagy is an intracellular degradation system that delivers cytoplasmic constituents to the lysosome and it's involved in the cycle of matter and energy. Meanwhile autophagic cell death is a form of cell death called type-Ⅱ programmed cell death. In recent years, studies have shown that autophagy is involved in pathophysiologies of nervous system disease especially neurodegenerative diseases, ischemia-hypoxia, traumatic brain injury ,seizures.

MicroRNAs(miRNAs)are a series of short non-coding RNAs that can regulate the expressions of protein-coding genes posttranscriptionally.They modulate gene expression by targeting mRNA for degradation or blocking translation via base-pairing to complementary sites in the 3'-untranslated region of the target mRNAs.Their functions are related to many biology progresses and diseases including tumor and hematopoietic differentiation.They are speculated to be critical in the development, progression and prognosis of leukemia. In this review, we will summarize the research progress on miRNAs in leukemia.

Humans ; Internal Fixators ; Lumbar Vertebrae ; surgery ; Spinal Fusion ; instrumentation ; methods ; Spinal Stenosis ; surgery

Asian Continental Ancestry Group ; Cardiovascular Diseases ; ethnology ; prevention & control ; Humans

Guanylyl cyelase-C (GC-C) is recently reported as the intestinal specific polypeptide receptor,which is expressed selectively by cells from intestinal mucosa,including normal intestinal cells,primary and metastatic colorectal cancer cells.Stimulated by heat-stable enterotoxin (STa),guanylin and uroguanylin,GC-C could not only regulate the proliferation and differentiation of intestinal cells,but also inhibit the proliferation of colorectal cancer cells and DNA synthesis.Micrometastases of the colorectal cancer can be found in lymph nodes,peripheral blood and liver lobes by GC-C detection.We can also perform GC-C and ligands for targeted prevention and therapy of colorectal cancer.GCC play important roles in detecting occult micrometastasos of colorectal cancer and targeted therapy.