Fracture Fixation ; Fracture Healing ; Humans ; Medicine, Chinese Traditional ; Radius Fractures ; therapy

Objective To investigate the feasibility of bedside temporary transvenous cardiac pacing with balloon tipped floating catheter under non X-ray fluoroscopy.Methods Twenty-four patients of bedside temporary transvenous cardiac pacing,11 cases underwent common electrode (common group),13cases underwent balloon tipped floating electrode (balloon tipped group).The operating time,pacing thresholds,pacing perception and complications were observed between two groups.Results All balloon tipped group placement success,8 cases in common group successfully.The operating time in balloon tipped group was significantly less than that in common group [(2.5 ± 1.5) min vs.(9.3 ±5.3) min],there was significant difference (P ＜ 0.05),but pacing thresholds and pacing perception between two groups had no significant difference (P ＞ 0.05).Common group occurred complications in 2 cases,respectively cardiac perforation and induced paroxysmal ventricular tachycardia,balloon tipped group occurred concurrent electrode wire dislocation in 2 cases.Conclusion Compared with the common electrode,balloon tipped floating electrode is simple,fast,high success rate and low risk of complications,suitable emergency physician bedside operating emergency temporary pacing.

Objective:To determine the prognostic value of DNA ploidy and nuclear morphometry,and its relations to nuclear grading in renal cell carcinoma.Methods:Pathological grading were done in 47cases of renal cell carcinoma in accordance with the system proposed by Fuhrman et al.Feulgen staining were performed in the tumor histological slides,and the technique of image cytometry was used to quantitate nuclear DNA ploidy and nuclear morphological parameters.The results combined with the data of clinical follow up were analyzed.Results:The mean DNA ploidy in grade Ⅰ～Ⅱ was mainly 2C,≥5C cells less than 10%;the 2C cells in grade Ⅲ～Ⅳ were lower than in grade Ⅰ～Ⅱ,≥5C cells were more than 20%.Among the four grades DNA ploidy were statistically different( P

80) via portal vein.After reperfusion 1 h,1 d,3d and 7 d respectively,the concentration of superoxide dismutase(SOD) and catalase(CAT) were tested,and the apoptosis of hepatocarcinoma also was observed using terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling(TUNEL) method.Results The results indicated that the SOD concentration in both hepatocarcinoma tissue and normal hepatic tissues decreased following I/R and perfusion with hyperoxic fluid liquid.The concentration of CAT increased following I/R in normal hepatic tissues.In hepatocarcinoma tissue,concentration of CAT decreased after reperfusion for 1 d and reached its lowest point.After perfusion with hyperoxic fluid,the concentration of SOD in both hepatocarcinoma tissue and normal hepatic tissues decreased more quickly following I/R and the low level was still found on 7 d after reperfusion.The concentration of CAT in tissues of both groups decreased and reached the lowest level at 1 h after reperfusion,but it was restored at 3 d reperfusion in normal hepatic tissues,and in hepatocarcinoma tissue was still at lower level until 7 d after reperfusion. After I/R,the apoptotic cells increased in normal hepatic and hepatic cancer tissues,and were most marked in tissues of hepatic carcinoma at 1 d and 3 d after perfusion with hyperoxic fluid.After I/R and perfusing with hyperoxic fluid,the changes of SOD and CAT and apoptosis in hepatocarcinoma tissue were more obvious than that in normal hepatic tissues(P

Ankle sprains are orthopedic clinical common disease, accounting for joint ligament sprain of the first place. If treatment is not timely or appropriate, the joint pain and instability maybe develop, and even bone arthritis maybe develop. The mechanism of injury of ankle joint, anatomical basis has been fully study at present, and the diagnostic problem is very clear. Along with the development of science and technology, biological modeling and three-dimensional finite element, three-dimensional motion capture system,digital technology study, electromyographic signal study were used for the basic research of sprain of ankle. Biomechanical and kinematic study of ankle sprain has received adequate attention, combined with the mechanism research of ankle sprain,and to explore the the biomechanics and kinematics research progress of the sprain of ankle joint.
Ankle Injuries ; physiopathology ; Ankle Joint ; physiopathology ; Biomechanical Phenomena ; Finite Element Analysis ; Humans ; Sprains and Strains ; physiopathology

OBJECTIVETo detect the levels of miR-499 and relative proteins in hearts of mice after exercise training, and investigate the mechanism of exercise-regulative apoptosis.

METHODSMale C57BL/6 mice were randomly divided into 3 groups( n = 14): sedentary (SE), exercise training 1 (ET1) and exercise training 2 (ET2) group. SE did not do any exercise. ET1 performed swimming training for 8 weeks. ET2 performed the same work as ET1 until the 5th week. Then, mice trained twice a day until the end of training. TUNEL assay was applied to test myocardial apoptosis, RT-PCR and Western blot were used to detect miR-499 and proteins levels respectively.

RESULTSCompared with SE, stress in ET1 failed to affect apoptotic index (AI) and miR-499-CaN-Drp-1 pathway (P > 0.05). In contrast, exercise load in ET2 increased miR-499 level, decreased Drp-1 level and AI with statistical significance respectively (P < 0.05), but neither CaN expression nor CaN activity was changed significantly (P > 0.05).

CONCLUSIONSwimming training can inhibit myocardial apoptosis, and the decrease in Drp-l may be responsible for the reduced myocardial apoptosis. CaN, the upstream protein, does not participate in exercise-regulative apoptosis.

Animals ; Apoptosis ; Dynamins ; metabolism ; Heart ; Male ; Mice ; Mice, Inbred C57BL ; MicroRNAs ; metabolism ; Mitochondria, Heart ; physiology ; Myocardium ; pathology ; Physical Conditioning, Animal ; Swimming

Objective To compare and analyze the difference of ?-tubulin and aurora-A expression in human cervical cancer cells (CasKi ) and immortalized human cervical squamous H8 cells with positive HPV16 E6E7.Methods Difference of ?-tubulin and aurora-A expression in CasKi and H8 cells was analyzed by showing the fluorescence intensity of ?-tubulin with indirect immunofluorescence.Expression level of aurora-A mRNA was detected by RT-PCR.Expression level of ?-tubulin and aurora-A in CasKi and H8 cells was semi-quantitatively analyzed by Western blot.Results The immunofluorescence signal of ?-tubulin was stronger in Caski cells than in H8 cells (57.78?3.13 vs 37.37?2.37,P

In order to fit new plot proposed by medical education reform,by integrating interrelated resources,the author introduced peer education into the whole process of nutrition propa-ganda and education to promote the development of the whole social health education.

In order to nurture the undergraduate majors of nutrition to fit society’s need,it is necessary to establish a new market-oriented talent training mode adaptable to both the market and society. This mode can also provide reference for the talent training of other emerging professions. Exploring the argument basis,theoretical design,practical development and research plan of this mode.

Objective To analyze the hospitalization rates of diabetic patients in different departments in different years and compare the differences in hospitalization costs, length of stay, and mortality between diabetic and non-diabetic patients. Methods The clinical data of hospitalized patients from 1995 to 2009 in different departments of Peking Union Medical College Hospital were retrospectively analyzed. Patients were divided into three groups by calendar period (every five years) and into four groups by ages. Results A total of 500 523 patients were enrolled in this study. From 1995 to 2009, the hospitalization rates of diabetic patients increased from 2.85% to 7.65% , especially among patients who were treated in the departments of cardiology, ophthalmology,and vascular surgery. In 2009, the hospitalization rates of diabetic patients in the three departments were 29.36% , 24. 51% , and 21.25 % , respectively. The length of hospital stay was significantly longer among diabetic patients than among non-diabetic patients (P ＜ 0. 001). The total hospital costs were also significantly higher among diabetic patients than among non-diabetic patients in all the period and age groups (P ＜ 0.01 or P ＜0.001)except in 2000-2004 group and 2005 - 2009 group in 0 - 18 years group (P ＞0. 05). The hospital cost per day in diabetic patients was significantly lower than that in non-diabetic patients in all the period and age groups (P ＜ 0. 05, P ＜ 0. 01 or P ＜ 0. 001)except in 1995-1999 group in over 65 years group (P ＞ 0.05). The mortality rate of diabetic patients in the 1995-2004 in 0-18 years group was zero, and the mortality rates were not significantly different between diabetic patients and non-diabetic patients in 2005 -2009 group in 0-18 years group (P ＞0.05), and were significantly higher in diabetic patients than in non-diabetic patients in all other period and age groups (P ＜0.01 or P ＜ 0. 001). Conclusions Diabetic inpatients tend to have a longer hospital stay, higher hospitalization rate, higher hospital costs, and higher mortality rates. A hospital-wide diabetes management mode should be developed to optimize its treatment.