1.Expression of leucocyte cell-surface antigens on colon cancer cell line HR8348.
Shi-long YANG ; Yon-min TANG ; Hong-qiang SHEN ; Bo-qin QIAN ; Hua SONG ; Shu-wen SHI ; Bo-tao NING ; Ying-hu CHEN ; Wei-qun XU
Journal of Zhejiang University. Medical sciences 2004;33(2):118-120
OBJECTIVETo define the immune phenotype of colon cancer cells.
METHODSUsing a panel of 40 anti-human monoclonal antibodies (MoAbs), the cells of colon cancer HR8348 were analyzed with three-color flow cytometry after direct immunofluorescent staining.
RESULTSHR8348 cell line did not express CD2, CD3, CD4, CD5, CD7, CD8, TCR, CD10, CD11b, CD14, CD16, CD19, CD22, CD25, CD28, SmIg, CD33, CD35, CD36, CD41a, CD45, CD45RA, CD45RO, CD56, CD61, CD64, CD66b, CD69, CD71, CD117, CD122 and P-glycoprotein but expressed CD13, CD15, CD20, CD38, CD95 and HLA-DR.
CONCLUSIONThe results demonstrate that colon cancer cell line HR8348 shares some antigenic determinants with leucocyte lineage.
ATP-Binding Cassette, Sub-Family B, Member 1 ; analysis ; Antigens, CD ; analysis ; Cell Line, Tumor ; Colonic Neoplasms ; chemistry ; Humans
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