No abstract available.
Intercostal Nerves*

No abstract available.

No abstract available.
Immobilization* ; Rib Fractures* ; Ribs*

Epstein-Barr virus (EBV) is a potent inducer of polyclonal B lymphocyte proliferation and a tool for the establishment of human B lymphoblastoid cell lines (B-LCLs), which have proven useful for several human immunologic applications. B-LCLs serve as efficient antibody-producing cells and antigen-presenting cells. In spite of these advantages, the cloning efficiency of B-LCLs is less than 1%. In order to generate clones of B-LCLs, we cultured B-LCLs with and without canine stromal cell line, DO64, as feeder cell which was immortalized by transduction of retrovirus encoding E6 and E7 of the human papilloma virus type 16 (HPV-16), which was defined to produce various cytokines including stem cell factor (SCF) and interleukin- 6 (IL-6). After 3 weeks of B-LCLs cultured with DO64, 8.3% and 37.5% in 1 cell and 3 cells per well were efficiently cloned, respectively. There was no significant effect on growing of 8-LCLs without DO64 cells and on high concentration of FBS. The cloning efficiency of B-LCLs transduced by retrovirus cultured with and without DO64 cells was 4.2% and 0% in 3 cells per well, respectively, while that of stable transfectant 33.3% and 8.3% in 1 cell per well, respectively. Our results suggest that the use of DO64 cells as feeder cells might permit the cloning of B-LCLs. This efficient cloning of B-LCLs could be used for the convenient source of autologous antigen-presenting cells expressing foreign antigen for the study of human immune responses in vitro, and for a variety of additional purposes, such as the production of human monoclonal antibodies.
Antibodies, Monoclonal ; Antibody-Producing Cells ; Antigen-Presenting Cells ; Cell Line* ; Clone Cells* ; Cloning, Organism* ; Cytokines ; Feeder Cells* ; Herpesvirus 4, Human ; Humans* ; Lymphocytes ; Papilloma ; Retroviridae ; Stem Cell Factor ; Stromal Cells*

The propolis, honey bee hive product, is a folk medicine for treating various ailrnents and caffeic acid phenethyl ester (CAPE) is an extract of propolis. The purpose of this study was to examine the effects of ethanol extracted propolis (EEP) or CAPE on the tumorigenesis, pulmonary metastases, and proliferation and activity of splenocytes and macrophages in ICR mice. EEP at 0.2, 2 or 20mg/ml applied topically on the back of each mice 30 minutes before application of 7,12-dimethylbenz (a)anthracene and 12-0-tetradecanoylphorbol-13-acetate inhibited the number of tumors per mouse by 61, 75 or 100%, respectively. ...continue...
Animals ; Bees ; Carcinogenesis* ; Ethanol ; Honey ; Macrophages* ; Medicine, Traditional ; Mice* ; Mice, Inbred ICR ; Neoplasm Metastasis* ; Propolis*

The Halo-pelvic traction has been considered as a powerful and strong method for correction of the severely deformed rigid spine whether it is kyphotic or scoliotic. It also affords firm stabilization during the operation and postoperative management with immobilization for a long time. Ambulation with this instrument enhances the usefulness. We have succeeded in treating and correcting 6 cases of Tuberculous Kyphosis, and 3 of which accompanied paraplegia. Especially one case of severe kyphosis, 78 was corrected to 20 and anterior strut graft was accomplished with vascuiarized free fibular graft with aid of microvascular technique. The correction rate was 38% in average. No significant complications have developed except osteoporosis of the spine.
Congenital Abnormalities ; Immobilization ; Kyphosis ; Methods ; Osteoporosis ; Paraplegia ; Spine ; Spondylitis ; Traction ; Transplants ; Walking

Of 62 supracondylar fractures of the humerus in children treated for 10 years, 27 cases are described with follow-up study of 2 years and 6 months on average. These patients were treated percutaneous K-wires fixation after closed reduction. This method takes advantage in stability of fixation, vascular safety with any permissible any position of the elbow, early ambulation and short hospital stay, and low incidence of varus deformity in follow-up. Complication included transient ulnar sensory change, pin-tract infection and inaccurate position of K-wires. We observed 1 case of clinical cubitus varus among 17 cases during more than one-year follow-up and concluded this unhappy result was associated with imperfect initial reduction rather than growth disturbance or initial displacement.
Child ; Congenital Abnormalities ; Early Ambulation ; Elbow ; Follow-Up Studies ; Humans ; Humerus ; Incidence ; Length of Stay ; Methods

Since 1957, multiple possible mechanisms have been suggested to explain the production of the osteonecrosis of the femoral head and it is now accepted that steroid medication is one of the etiologic factors producing osteonecrosis of the femoral head. Among the surgical procedures in ischemic osteonercrosis of the femoral head are bone grafting, core decom-pression, rotational osteotomy, angulation osteotomy, hemiarthroplasty and total hip replacement. The choice of surgical procedures in the management of idiopathic osteonecrosis of the femoral head is dependent on several factors. Most important are the etiology and stage of the lesion. Other considerations are the patients age, lifestyle, and demands on his hip, and the surgeons preference and expertise. We reviewed 32 total hip replacements in 22 patients, which had been performed to treat steroid-induced osteonecrosis of the femoral head at Department of Orthopaedic Surgery, National Medical Center from Jan. 1975 to Dec. 1981. The longest follow-up was 7 years and 6 months and the shortest, 1 year, the average being 2 years and 6 months. The results were as follows: 1. There were 13 males and 9 females and more than half were in 5th decade. 2. Principal causes to use oral corticosteroids were rheumatoid arthritis, arthralgia, and low back pain in the order of frequency. 3. Duration of corticosteroid medication was between 2 months and 12 years and 18 patients took more than 12 months. 4. Of 18 patients who had the bilateral involvement, the two also had osteonecrosis of the humeral head. 5. Roentgenographically, 30 hips were in stage IV and 2, in stage III according to the classification of Arlet and Ficat. 6. Types of the implants used were Charnely (28 cases), MQller (2 cases) and Trapezoidal-28 (3 cases). 7. The function of the each hip was evaluated preoperatively and postoperatively by the method of Wilson and Salvati and all improved from average poor to average good.
Adrenal Cortex Hormones ; Arthralgia ; Arthritis, Rheumatoid ; Arthroplasty, Replacement, Hip ; Bone Transplantation ; Classification ; Female ; Follow-Up Studies ; Head ; Hemiarthroplasty ; Hip ; Humans ; Humeral Head ; Life Style ; Low Back Pain ; Male ; Methods ; Osteonecrosis ; Osteotomy ; Surgeons

Despite its benign nature, chronic subdural hematoma (SDH) can be fatal if surgical intervention is delayed. Here, we report on bilateral chronic SDH in an 84-year-old man who died of duret hemorrhage in the brain stem and ischemia in the occipital and temporal lobes. We discuss the necessity for urgent surgical intervention to treat bilateral chronic SDH, and provide a review of the relevant literature.

During the period from June 1st 1971 to November 30th 1971, studies on air polluton were made in Kwangju city. The city was divided into 6 areas : the downtown ares, the simi-downtown area, the heavy traffic area, the commercial area, the residential area, the park area, 13 surveying sites were selected each representing the characteristics of the area. The Measurement methods which were used are described below. Sulfur oxides were measured by PbO2 cylinder method, sulfur dioxides (SO2) and carbon monoxide (CO) by the MSA & Kitakwa detector, dustfall by the Deposit gauge method, and the noise levels by the Kanomax sound level meter. The results obtained are as follows : 1. The mean value of sulfur oxides in Kwangju city was 1.16 mg SO3/day/100cm2 PbO2, ranging from 0.45mg SO3/day/100cm2 PbO2 to 3.10mg SO3/day/100cm2 PbO2. 2. The mean value of sulfur oxides according to its specific area in the city were 1.45mg SO3/day/100cm2 PbO2 in heavy traffic area, 1.36 in downtown area, 1.23 in semi-downtown area, 1.11 in commercial area, 0.96 in residential area, and 1.07 in park area, respectively. 3. The average concentration of sulfur dioxide was 0.063 ppm from 2 to 5 P.M in Kwangju city. 4. The average concentration of sulfur dioxides according to its specific area, from 2 to 5 P.M, in the city were 0.084 ppm in heavy traffic area & downtown area, 0.067 in commercial area, 0.053 in semi-downtown area, 0.052 in residential area, and 0.036 in park area. 5. The average concentration of carbon monoxide was 22.3 ppm from 2 to 5 P.M in Kwangju city. 6. The average concentration of carbon monoxide according to its specific area, from 2 to 5 P.M, in the city were 27.0ppm in downtown traffic area, 26.3 in semi-downtown area, 23.0 in heavy traffic area, 21.7 in commercial area, 20.0 in residential area, and 17.6 in park area. 7. The mean value of dustfall in Kwangju city was 29.28 ton/Km2/month, ranging from 9.85 ton/Km2/month to 66.34 ton/Km2/month. 8. The mean values of dustfall according to its specific area in the city were 50.37 ton/Km2/month in semi-downtown area, 42.76 in heavy traffic area, 34.67 in downtown area, 17.77 in commercial area, 14.40 in park area, and 14.76 .in residential area. 9. The mean value of the soluble dust in Kwangju city was 10.23 ton/Km2/month and that of the insoluble dust was 19.05 ton/Km2/month. 10. The mean values of noise level in Kwangju city was 62 phon, ranging from 37phon to 88 phon. 11. The mean values of noise level according to its specific area in the city were 76 phon in heavy traffic area, 67 in semi-downtown area, 64 in downtown area, 59 in commercial area, 52 in part area, and 50 in residential area.
Air Pollution* ; Carbon Monoxide ; Dust ; Gwangju* ; Noise* ; Sulfur ; Sulfur Dioxide ; Sulfur Oxides