OBJECTIVE:To study the effects of ulinastatin on pathological state of lung tissue and aquaporin 1(AQP-1)and matrix metalloproteinases (MMP-2),MMP-9 expressions in silicosis rats. METHODS:Wistar rats were divided into normal con-trol group,model group,ulinastatin high-dose,medium-dose,low-dose groups(50×104,10×104,2×104 u/kg),20 in each group. Except for normal control group,rats in other groups were given 40 mg/mL SiO2 suspension 1 mL by non-exposed endotracheal per-fusion to induce silicosis model;1 h before modeling administration,corresponding doses of ulinastatin were intraperitoneally in-jected,for 3 d. Hematoxylin-eosin staining was used to observe silicon nodules in lung tissue of rats in each group;immunofluores-cence method was used to detect AQP-1 expression in lung tissue;real-time fluorescence quantitative polymerase chain reaction method was used to detect MMP-2,MMP-9 mRNA expressions in lung tissue;Western blot method was used to detect MMP-2, MMP-9 protein expressions in lung tissue. RESULTS:There were obvious silicon nodules and serious structural damage in lung tis-sue in model group,ulinastatin groups showed macrophage foci visible dust,but pulmonary fibrosis was obviously reduced. Com-pared with normal control group,AQP-1 expression in lung tissue in other groups were reduced,MMP-2,MMP-9 mRNA and pro-tein expressions were enhanced (P<0.05). Compared with model group,AQP-1 expression in lung tissue in ulinastatin groups were increased,MMP-2,MMP-9 mRNA and protein expressions were decreased (P<0.05),in which improvement effects in high-dose,medium-dose groups were superior to low-dose group(P<0.05). CONCLUSIONS:Ulinastatin can reduce the patholog-ical state of silicosis rats,increase AQP-1 expression and decrease MMP-2,MMP-9 expressions.

BACKGROUND:Cel transplantation becomes a new approach for treatment of cerebral infarction. In recent years, bone marrow mesenchymal stem cels (BMSCs) have become an important kind of seed cels in cel transplantation.
OBJECTIVE: To investigate the effect of fleabane injection combined with BMSC transplantation on S100B protein and superoxide dismutase expression in acute cerebral infarction rats.
METHODS:Animal models of acute cerebral infarction were made in Sprague-Dawley rats using suture method. After successful modeling, 80 model rats were randomly divided into control group, fleabane group, BMSC group and combined group (fleabane combined with BMSC transplantation). Changes of serum S100B protein and serum superoxide dismutase levels were detected using enzyme-linked immunosorbent assay and xanthine oxidase method, respectively, before and after treatment. NIHSS neurological function scores were measured to observe neurological behavior changes in model rats. The infarct volume was measured by TTC staining.
RESULTS AND CONCLUSION:At 36, 7, 14 days after treatment, S100B protein levels in the fleabane group and BMSC group were significantly lower than that in the control group, but higher than that in the combined group (P <0.05); serum superoxide dismutase levels in the fleabane group and BMSC group were significantly higher than that in the control group, but lower than that in the combined group (P < 0.05). At 1, 2, 3 weeks after treatment, NIHSS neurological function scores were ranked as folows: combined group < fleabane group and BMSC group < control group, and there was a significant difference between groups (P < 0.05). At 2 weeks after treatment, the infarct volume in the fleabane group and BMSC group was higher than that in the combined group but lower than that in the control group (P < 0.05). These findings indicate that fleabane combined with BMSC transplantation can inhibit the expression of S100B protein in rats with acute cerebral infarction, and promote the activity of superoxide dismutase, thereby playing a neuroprotective role.

BACKGROUND:Studies have shown that tanshinone II can improve microcirculation, dilate cerebral blood vessels, increase cerebral blood flow, reduce infarct size, and improve brain function after cerebral metabolic disorder.
OBJECTIVE:To investigate the effect of tanshinone II combined with bone marrow mesenchymal stem cel transplantation on nerve regeneration folowing cerebral infarction in rats.
METHODS:Rat models of acute cerebral infarction were prepared using the thread occlusion method and then given tanshinone II combined with bone marrow mesenchymal stem cel transplantation (combined group), bone marrow mesenchymal stem cel transplantation (cel transplantation group), and no treatment (model group), respectively. Neuromotor function was assessed using Longa scores. Expression of brain-derived neurotrophic factor gene around the infarction region was detected using RT-PCR. Cel apoptosis was detected using TUNEL. Immunohistochemistry staining was used to determine peri-cortex Nogo-A and NgR protein expression at the infarction region.
RESULTS AND CONCLUSION:Longa scores, apoptotic index, and expression of Nogo-A and NgR proteins exhibited significant differences among three groups (P< 0.05) as folows: combined group < cel transplantation group < model group. Conversely, the expression of brain-derived neurotrophic factor gene was highest in the combined group successively folowed by the cel transplantation group and model group (P< 0.05). These data show that tanshinone II combined with bone marrow mesenchymal stem cel transplantation accelerate the recovery of neurologic function and promote nerve regeneration after cerebral infarction by incresing mRNA expression of brain-derived neurotrophic factor.

AIM: To investigate the effect of combination of traditional Chinese and western medicine treatment on active tuberculosis patients and the impact on peripheral blood T-lymphocyte subsets. METHODS: 133 cases of active pulmonary tuberculosis patients were divided into two groups.The clinical effects of two groups were compared and Tlymphocyte subsets were detected. RESULTS: The total effective rate was 95.8%,its efficacy was superior to the control group(P

Objective To study the effects of ShaJi on the indexes of oxygen metabolism such as coronary blood flow in myocardium of anesthetized thoraco-opened dogs. Methods Dogs were randomly divided into control group,4 and 16 mg?kg-1 ShaJi groups,and positive control group(n=6).The anesthetized thoraco-opened dog models were set up.The administration of intravenous injection was used by femoral vein.The blood pressure,heart rate and coronary blood flow(CBF) were measured.Coronary resistance,myocardial oxygen uptake rate,myocardial oxygen consumption index and myocardial oxygen consumption were calculated.Results Compared with control group,the CBF was increased (P0.05).Conclusion ShaJi can significantly ameliorate oxygen metabolism in myocardium of anesthetized thoraco-opened dogs.

Anti-tumor necrosis factor-α(anti-TNF-α)agents have been widely used in the treatment of inflammatory bowel disease(IBD)in children.Anti-TNF-α therapy can effectively induce and maintain disease remission, promote intestinal mucosal healing, and prevent long-term end-stage organ damage and growth retardation in pediatric IBD patient.Anti-TNF-α agents can significantly impair the human immune function, which may increase the infection risk of IBD children, including the infection of bacteria, viruses, fungi and mycobacteria.This study summarizes the current published literature regarding infections in pediatric patients with IBD receiving anti-TNF-α therapies, which can help to improve the cognition of pediatric medical staff on opportunistic infection of pediatric IBD patients following anti-TNF-α treatment.

Objective:To analyse the skin clinical characteristics of adverse reactions to cosmetic products.Methods:A total of 132 patients suffered with the skin adverse reactions of cosmetics were collected in the Department of Dermatology in the First Affiliated Hospital of Xinxiang Medical University from January 2021 to December 2021. There were 5 males and 127 females, aged 2-66 (34.0±13.1) years. and the personal information, medical records, clinical characteristics and the cosmetic information as well as laboratory results were collected.Results:The major types of adverse reactions to cosmetic products were contact dermatitis (86.4%). Head was the most commonly affected site, The most common symptoms were pruritus and burning sensation (83.9%), and sores, dryness, tightness of the skin, and the common skin lesions included erythema and papula (92.9%). Suspected cosmetics were mostly skin care products (45 cases) and freckle removing products (30 cases). Only 7 patients accepted patch tests, 1 case had negative results and others were all tested positive.Conclusions:Adverse drug reactions affect young and middle-aged women mostly. Contact dermatitis is the most common adeverse reaction to cosmetic products and the patch test is still the most effective method in helping diagnosing contact dermatitis to cosmetic products.

Objective To understand patient pathway and clinical characteristics of chronic diseases in urban areas of Shanghai. Methods A total of 10 002 residents were enrolled and assigned to the chronic disease group (including hypertension, diabetes, coronary heart disease, myocardial infarction, and ischemic stroke) and the non-chronic disease group. Body mass index,fasting blood glucose, triglyceride,total cholesterol, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol were tested.Difference of patient pathway and clinical characteristics of those chronic diseases was compared. Results Above chronic diseases were observed in 37.7% participants. About 2/3 diseases were confirmed and 80% patients were followed up in healthcare units not far away from home. Patients with coronary heart disease and myocardial infarction showed more outpatient visit to tertiary hospitals (P＜0. 05 ). However, patients with ischemic stroke had health check, rehabilitation and pharmacy done mainly in local healthcare centers (P＜0. 05 ). Diastolic blood pressure of patients visiting local doctors was significantly decreased (P＜0. 05). Conclusion Some differences in patient pathway were found in this study. Communication and cooperation between medical institutions should be intensified for effective chronic disease control.

ObjectiveTo analyze the effectiveness and safety of clinical use of different types of stem cells to provide evidence for governmental supervision of key issues in clinical utilization of stem cells. MethodsSix literature databases in China and abroad were searched for relevant literature published from January 2010 to July 2020， and a systematic review was conducted. ResultsThe study showed 72 studies concerning the effectiveness and safety of different types of stem cells in clinical utilization. Although clinical utilization of stem cells had some therapeutic effects for certain diseases， their long-term effect and safety need to be further evaluated， especially their potential risk of tumorigenicity. ConclusionTo protect the patients’ interest， physicians should fully weigh the benefits against the risks of clinical utilization of stem cells， and relevant governmental departments should strengthen supervision of ethics of clinical research and therapies involving stem cells as well as supervision of stem cell products.