"Adaptation of human body to natural environment"is one of the most important theories in Inner Canon of Huangdi(Huangdi Nei Jing).It explains some kinds of links between the natural variations and the changes of the human activity. This article analyses some comments about menstruation in Inner Canon of Huangdi from the angle of gynecologic pathology, physiology,treatment,health care as one side,and the variation of the moon,the four season,the day as another side.On the basis of that,this article explores applications of"adaptation of human body to natural environment"theory in gynecology.

This essay collects relative description on amenia in “Internal Classic” and explores its causa morbi, mechanism, diagnosis and treatment, also expounds the different methods to two types of amenia caused by blood stagnation and dry blood.

Objective] To discuss the diagnosis and treatment of infertility according to the related comments in. [Method] Analyzing the infertility related scripture in and carefully reading the related annotations to analyze and elaborate the etiology and therapeutic principles of infertility. [Result]suggested that the uterus, Jing from parents, Du Yang, Yin and Yang of kidney were essential to fertility. Deficiency of kidney and insufficient Du were main etiologies of infertility. < Huangdi Neijing > recommended that tonifying kidney and warming Du as treatments for infertility. [Conclusion]The theory about the etiology and therapeutic principles of infertility has taken shape in.is a cornerstone in the development of the etiology and therapeutic principles of infertility.

Objective:To investigate the mechanism of curcumin in the treatment of diabetic retinopathy (DR) by network pharmacology and molecular docking.Methods:The compounds targets of curcumin were predicted by SEA and SwissTargetPrediction databases, and the DR target genes were obtained by CTD database.The different genes were mapped and matched by Venny database to screen their intersections.The intersecting genes were submitted to GeneMANIA database to construct a protein-protein interaction network.WebGestalt database was used to conduct enrichment analysis and AutoDock Vina was used to perform molecular docking of the core targets.Results:A total of 52 targets of curcumin, 1 599 targets of DR and 48 intersecting targets were detected.The core targets were serine/threonine-protein kinase 1 (AKT1), tumor necrosis factor-α (TNF-α), epidermal growth factor receptor (EGFR), signal transduction and activator of transcription 3 (STAT3) and heat shock protein 90 alpha family class A member 1 (HSP90AA1). Enrichment analysis suggested that these targets were mainly associated with signaling pathways, including the EGFR tyrosine kinase inhibitor resistance signaling pathway, hypoxia-inducible factor-1 (HIF-1) signaling pathway, interleukin (IL)-17 signaling pathway and advanced glycosylation end product-the receptor of advanced glycosylation end product (AGE-RAGE) signaling pathway.Conclusions:Curcumin may play an important role in the treatment of DR by regulating multiple signaling pathways to inhibit the inflammatory response and combat oxidative stress.

Abstract OBJECTIVE To explore the potential targets and mechanisms of Rhodiolae Crenulatae Radix et Rhizoma in regulating autophagy in granulosa cells based on network pharmacology and experimental verification. METHODS With the help of the database, the active components, effect targets and autophagy-related targets of Rhodiolae Crenulatae Radix et Rhizoma were obtained, and a component-target network diagram and a protein-protein interaction network diagram were established. Enrichment analysis of genes and signaling pathways was conducted on key targets and the active ingredients and core targets were docked to explore the possible mechanism of Rhodiolae Crenulatae Radix et Rhizoma in regulating autophagy in granulosa cells. Western blotting, RT-PCR, transmission electron microscopy and double-labeled adenovirus were used to detect the effect of important active ingredients of Rhodiolae Crenulatae Radix et Rhizoma on autophagy in granulosa cells. RESULTS There were 7 active ingredients of Rhodiolae Crenulatae Radix et Rhizoma obtained, with 441 targets, 796 autophagy-related targets, and there were 834 biological processes, 60 cellular components, 69 molecular functions, and 150 signaling pathways obtained by enrichment analysis. Molecular docking results showed that the active components of Rhodiolae Crenulatae Radix et Rhizoma and the core targets AKT1, ERBB2, PIK3CA, PIK3R1 could form a stable target binding effect. The experimental results showed that salidroside, an important active component of Rhodiolae Crenulatae Radix et Rhizoma, could promote the formation of autophagosomes and autophagolysosomes, and inhibit the activity of the Pl3K/AKT signaling pathway. CONCLUSION The results show that the regulation of autophagy in granulosa cells by Rhodiolae Crenulatae Radix et Rhizoma has the characteristics of multi-component-multi-target-multi-pathway, and its mechanism is related to the regulation of PI3K/AKT signaling pathway by salidroside.