Objective To study the detection and clinical significance of C reaction protein(CRP)in patients with ulcerative colitis(UC).Methods The levels of peripheral blood CRP and blood platelets count(BPC)in patients with UC(n=57) and normal controls group(n=30)were detected and the effects on disease severity were analyzed subsequently.Results The levels of peripheral blood CRP and BPC in active phase group were significantly higher than those in catabasis group(P＜0.05),they were no significant difference between catabasis group and norreal controls group(P＞0.05);the leveh of peripheral blood CRP and BPC in severe stage were significantly higher than those in medium stage(P＜0.05),in medium stage,severe stage they were significantly higher than those in mild stage(P＜0.05,P＜0.01).Conclusion The level of peripheral blood CRP and BPC had important value to judge active phase and severity of UC.

Objective To investigate the effect of esomeprazole in the treatment of digestive ulcer with heli- eobacter pylori(Hp)infection.Methods A total of 60 patients with digestive ulcer and Hp infection were randomly divided into group A(30 cases)treated with esomeprazole(20mg),amoxicillin(1000mg)and clarithromycin(500 mg)twice daily and group B(30 cases)treated with omeprazole(20mg),amoxicillin(1000mg)and clari thromycin (500mg)twice daily.The treatment lasted for 7 days,after which abdominal pain refief rate,ulcer healing rate and eradication rate of Hp as well as adverse effects of the medication were evaluated and compared.Results Ulcer heal- ing rate and Hp eradication rate were 90.1% and 94,2 % in group A and 83.9 % and 83.7 % in group B,showing significant statistical diferences between the two groups(P

Cell senescence is a state of irreversible growth arrest, which can be triggered by a variety of different cellular stress. Currently, the detection indexes involved in the study of cell senes?cence include senescence-associated β-galactosidase, telomeres and telomerase, senescence-associ?ated heterochromatin foci, senescence-associated secretory phenotype, reactive oxygen species, and tumor suppressor genes p53 and p16. These indexes are widely used in the study of cell senescence, each with its own characteristics or advantages. This review summarizes several common cell senes?cence indexes and compares their accuracy, credibility, specificity, and potential applications.

Objective To explore the applied value of combined measurement of cystatin c (Cys-C) ,fibrinogen(Fbg) and U-mALB in early renal damage due to diabetes .Methods 97 cases of type Ⅱ diabetes mellitus (T2DM ) patients were divided into 2 groups according to the measured glycated hemoglobin HbA 1c ,serum glucose well control group (46 cases ,HbA1c≤8% ) ,and ser-um glucose poor control group (51 cases ,HbA1c>8% ) .39 healthy people were randomly selected as control group .The concentra-tion of Cys-C ,Fbg and U-mALB were detected .Results Cys-C ,Fbg and U-mALB of serum glucose poor control group were higher than that of glucose well control group and control group (P<0 .01) .Cys-C and U-mALB of glucose well control group were high-er than the control group (P<0 .01) .As a single positive marker of diagnosis ,the sensitivity for serum Cys-C ,serum Fbg ,and U-mALB were 73 .6% ,71 .4% and 61 .5 % respectively .The positive rate of combined detection was 97 .0% ,which was significantly higher than that of the individual testing (P<0 .05) .Conclusion Combined measurement of Cys-C ,Fbg and U-mALB has impor-tant clinical value for diagnosis and condition monitoring in early renal damage .

Objective To explore the features of sex hormones and insulin resistance and to analyse the relation between these features and ultrasonographic data in patients with polycystic ovary syndrome(PCOS) .Methods 120 patients with PCOS diagnosed and treated in hospital from April 2010 to December 2013 were enrolled as PCOS group ,and 50 healthy persons as control group ,in both groups ,ovarian stromal area(SA) ,ovarian total area(TA) and SA/TA ratio were measured by ultrasonography ,and serum levels of follicle stimulating hormone(FSH) ,luteinizing hormone(LH) ,LH/FSH ratio ,estradiol(E2) ,testosterone(TESTO) ,and prolactin(PRL) were assayed .Fasting plasma glucose(FPG) and fasting insulin(FIN) were measured ,and homeostasis model as‐sessment for insulin resistance(HOMA‐IR)was calculated in the PCOS group .Results the serum levels of LH ,LH/FSH ratio , TESTO ,SA ,and SA/TA ratio in the PCOS group were higher than that of the control group .SA and SA/TA ratio were correlated positively with LH ,LH/FSH ,TESTO ,and HOMA‐IR in the PCOS group .Conclusion sex hormones and insulin resistance were correlated with ultrasonographic data in the PCOS patients .

[Objective] To study the effect of celecoxib on osteoblast apoptosis induced by titanium particle in neonatal rats. [Methods]The best in vitro celecoxib serum concentration for promoting proliferation of osteoblast in neonatal rats was obtained through MTT.One group of rat osteoblast in vitro was cultured with serum containing saline and titanium particle and the other was cultured with serum containing celecoxib and titanium particles.Concentrations of TNF-? and PGE-2 in cells collected after 24 hours were analyzed by ELLSIA,COX-2 mRNA and protein were indicated by FO-PCR and Westren blot.[Results]Some cellar factors such as COX-2,TNF-? and PGE-2 could be induced by titanium particle in in vitro,but celecoxib could inhibit expression of these factors by COX-2 pathway.[Conclusion]Celecoxib coule be a promising drug for treatment of aseptic loosening.

Functional magnetic resonance imaging (fMRI) plays an ever increasing role in human brain function research with its development. To make an analysis of the MRI data and draw the conclusion, it is necessary to deeply understand the formulation of the signal and collection of the data of image. We introduce in this paper the data signal of magnetic resonance imaging and its noise, the analysis of the experiment data. New development of data analysis for fMRI is reviewed.

BACKGROUND: Pseudoachondroplasia is rare heredigary osteopathy, usually accompanied by deformity of the lower limbs, short fingers, and ligamentous laxity, so it is a difficulty in total knee arthroplasty.OBJECTIVE: To evaluate the curative effectiveness of bilateral total knee arthroplasties for a patient with pseudoachondroplasia using three-dimensional (3D) printing technology.METHODS: A retrospective study was done in a case of pseudoachondroplasia undergoing bilateral total knee arthroplasties using 3D printing technology. X-ray examination was conducted to observe the prosthesis location after arthroplasties, and the Knee Society Score outcome measures were used to evaluate the functional outcome.RESULTS AND CONCLUSION: (1) After arthroplasties, X-rays showed good line of force of the lower limbs and prosthesis location, and 1 year later, showed no radiolucent lines in tibia. (2) The right Knee Society Score improved to 94 points, the functional scoring improved to 90 points, and the postoperative flexion was 95°; the left Knee Society Score improved to 93 points, the functional scoring improved to 90 points, and the postoperative flexion was 90°. All patients were very satisfied with the treatment outcomes. (3) These results indicate that the individualized treatment scheme designed using 3D printing technology can reduce the surgical difficulty and trauma with high accuracy, and promote the functional recovery of the knee. Additionally, it obtains good clinical efficacy and patient's satisfaction in orthopaedic surgeries.

BACKGROUND:Previous studies have shown that apoptosis, a central feature of articular chondrocytes, plays a dominant role in cartilage damage, which is one of the pathological factors of articular cartilage degeneration. OBJECTIVE:To observe the effects of meditcated serum containingDuhuojisheng Decoction on the expression of cytochrome C, proCaspase-9 and proCaspase-3 in rat degenerative chondrocytes in vitro and to investigate the possible molecular biological mechanism ofDuhuojisheng Decoction in the treatment of knee osteoarthritis. METHODS:A cultivation system of degenerative chondrocytes in vitro was established. After treatment with meditcated serum containingDuhuojisheng Decoction or blank serum for 24 and 48 hours, the protein expression of cytochrome C, proCaspase-9 and proCaspase-3 was measured by western blot assay. RESULTS AND CONCLUSION: In the cytoplasm, the release of cytochrome C was reduced gradualy in both groups in a time-dependent manner, and the release amount of cytochrome C was significantly lower in the medicated serum group than the blank serum group (P < 0.05). In mitochondria, cytochrome C leakage was gradualy decreased in both groups, and it was decreased significantly in the medicated serum group compared with the blank serum group (P < 0.05). The protein expression of proCaspase-9 and proCaspase-3 was gradualy increased in both groups, especialy in the medicated serum group; the medicated serum containingDuhuojisheng Decoction could promote the protein expression of proCaspase-9 and proCaspase-3 in a time-dependent manner, and there was a significant difference at 24 and 48 hours (P< 0.01). These findings indicate that the medicated serum containingDuhuojisheng Decoction can inhibit the apoptosis of osteoarthritis chondrocytes through inhibiting the release of cytochrome C and the activation of Caspase-9 and Caspase-3.

BACKGROUND:Due to variation of spinal anatomic structure, vertebral body rotation, absence of vertebral body, and spinal canal deformation in patients with scoliosis, the deviation of pedicle screw placement can induce catastrophic results. Recently, with the wide application of pedicle screw and the continuous development of computer navigation, scoliosis surgery becomes more accurate and safe.
OBJECTIVE:To review the existing problems and advantages of Iso-C arm three-dimensional (3D) navigation system for guiding internal fixation of pedicle screw implantation in the treatment of scoliosis.
METHODS: The first author searched PubMed (http://www.ncbi.nlm.nih.gov/PubMed) and CNKI (http://www.cnki.net/) for articles published until December 2012. The key words were “computer navigation, scoliosis, pedicle screw”. The literatures with abstracts containing above key words were retrieved. Totaly more than 50 articles were found, and 13 articles were accorded with the inclusion criteria.
RESULTS AND CONCLUSION: Iso-C arm 3D navigation system for guiding internal fixation of pedicle screw implantation in the treatment of scoliosis had obvious advantages. It fuly combined the advantages of CT 3D navigation and intraoperative X-ray navigation, and can quickly and accurately capture the image data. Real time display of images could ensure the safety of repair operation. Automatic registration function of Iso-C arm 3D navigation undoubtedly improves the efficiency and accuracy of the repair, and helps physicians with more accurate and safe ways to carry out a variety of complex scoliosis surgery.