1.Flow Cytometry and Immunohistochemical Study of Psoriasin in the Skin Lesion of Psoriatic Patients
Qing SUN ; Chunyang LI ; Yiyuan GUO ;
Chinese Journal of Dermatology 1994;0(06):-
ObjectiveToinvestigatetheroleofpsoriasininthepathogenesisofpsoriasis.MethodsImmunohistochemicaltechnique(SABC)andflowcytometrywereemployedtodetecttheexpressionofpsoriasinin14patientswithpsoriasis.ResultsTheexpressionofpsoriasinwasup-regulatedinpsoriatickeratinocytesandtheamountofitsexpressionwas23.38%?4.49%.Thepresenceofpsoriasinwaslocatedinthebasalandsuprabasallayersofpsoriaticlesions.Theepidermisinnormalcontrolsdidnotexpresspsoriasin.ConclusionTheoverexpressionofpsoriasininpsoriaticepidermissuggeststhatpsoriasinbeoneofthefactorsleadingtotheinfiltrationofinflammatorycellsinpsoriaticepidermisandberelatedtothehyperproliferationofpsoriatickera-tinocytes.
2.Direct activation of expression vector withβ-casein gene promoter using engineered TALE transcription factors in mouse fibroblasts
Wenhui PI ; Long LIANG ; Hong TANG ; Yiyuan ZHANG ; Yanhua GUO ; Limin WANG ; Chunhe XIANG ; Ping ZHOU ; Shouren LIU
Acta Laboratorium Animalis Scientia Sinica 2014;(5):13-16
Objective TALE-TFs were adopted to provide a new way in detection of the expression result ofβ-ca-sein gene promoter-interesting gene expression cassettes in mouse fibroblasts.Methods TALE-TFs of eukaryotic expres-sion plasmid and expression cassette withβ-casein gene promoter and red fluorescent protein reporter gene were co-nucleo-fected into mouse fibroblasts by Amaxa nucleofector.Results and Conclusion β-casein gene promoter was activated by artificial TALE-TFs in the mouse fibroblasts.The way is a new expression verification system instead of mammary epithelial cells with fibroblasts.
3.Analysis of genetic variants in a pedigree affected with hereditary multiple osteochondroma.
Xiaoyan GUO ; Qinqin ZHENG ; Mingrui LIN ; Yiyuan ZHANG ; Tengfei SHI
Chinese Journal of Medical Genetics 2021;38(6):549-552
OBJECTIVE:
To explore the genetic basis for a pedigree affected with hereditary multiple osteochondroma (HMO).
METHODS:
Peripheral blood samples were collected from the proband and members of his pedigree with informed consent. Following extraction of genomic DNA, all coding exons and flanking intronic sequences (-10 bp) of the EXT1 and EXT2 genes were subjected to targeted capture and next generation sequencing (NGS). Suspected variant was verified by Sanger sequencing.
RESULTS:
A heterozygous nonsense variant (c.1911C>A) was found in exon 10 of the EXT1 gene in the proband and his affected father but not in a healthy sister and normal controls. The variant was classified as a pathogenic based on the guidelines of the American College of Medical Genetics and Genomics (PVS1+PM2+PP1). Bioinformatic analysis predicted that the c.1911C>A variant may be disease-causing via nonsense-mediated mRNA decay and anomalous splicing.
CONCLUSION
The c.1911C>A variant probably underlay the disease in this pedigree. Discovery of this variant enriched the variant spectrum of HMO.
Codon, Nonsense
;
Exons/genetics*
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Exostoses, Multiple Hereditary/genetics*
;
Heterozygote
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Humans
;
Pedigree
4.Application of phage in patients with urinary tract pandrug-resistant Klebsiella pneumoniae infection
Yigang ZENG ; Juan BAO ; Demeng TAN ; Yiyuan ZHANG ; Mingquan GUO ; Zhe ZHU ; Enming SHAO ; Tongyu ZHU
Chinese Journal of Urology 2020;41(9):677-680
Objective:To summarize the application of phage therapy in patients with urinary tract complicated pandrug-resistant Klebsiella pneumoniae infection, and analyze its feasibility and effectiveness.Methods:To retrospectively analyze the clinical data of a patient with complicated urinary tract complex pan-resistant Klebsiella pneumoniae treated by phage from August to September, 2019 in Shanghai Public Health Clinical Center. The female patient, 65 years old, was admitted to the hospital on August 6, 2020. The patient repeated with frequent micturition and urgent micturition half a year before admission. These symptoms were not accompanied by back pain, fever, chills, dysuria, gross hematuria. Urinary culture results in outpatient hospital was pan-resistant Klebsiella pneumoniae. After the patient discontinued application of cefoperazone sulbactam, levofloxacin and other drugs, symptoms such as frequent urination could be relieved after treatment, but appeared repeatedly. In August 2019, the center innovatively applied phage therapy to treat this patient with urinary tract pandrug-resistant bacteria infection.Results:For the first time, we applied 117, 135, 178, GD168 phage mixed solution once a day, for 5 days of continuous bladder infusion. At the same time, meropenem and amikacin was intravenous administration to strengthened the anti-infection treatment. Urine culture was negative for two consecutive times after treatment. However, half a month after the end of the bladder infusion, the patient experienced discomfort such as frequent urination. Urine culture: pan-resistant Klebsiella pneumoniae. The second time, we applied a mixture of three phage strains 130, 131, 909, once a day, for 5 days of continuous bladder infusion. And in the afternoon of the third day of treatment, the renal pelvis was retrogradely intubated and perfused with the above three strains of phage mixture. During the second treatment follow-up until March 30, 2020, the patient's urine culture was reviewed once a month. As a result, no pan-resistant Klebsiella pneumoniae was found, and the patient no longer experienced frequent urination and other symptoms of urination. The treatment process was successful and without severe complications and side effects.Conclusions:Phage urinary tract perfusion is an effective method for the treatment of pan-resistant Klebsiella pneumoniae urinary tract infections. The curative effect is accurate and reliable. The patient did not show obvious complications and adverse reactions during treatment. It can be used as an alternative treatment plan for complex pan-resistant Klebsiella pneumoniae infection.
5.Feasibility of corneal epithelial transplantation with polyethylene glycol hydrogel membrane as a carrier for limbal stem cell deficiency
Yiyuan GUO ; Huimin XIAN ; Tan SHEREEN ; Fu QIANG ; Xin JIN ; Daniel MARK ; Qiao GREG.G. ; Hong ZHANG
Chinese Journal of Experimental Ophthalmology 2022;40(12):1125-1133
Objective:To investigate whether polyethylene glycol hydrogel films (PHFs) can be used as a carrier for the expansion of corneal epithelial cells (CECs) in vitro and whether PHFs can be used in the treatment of limbal stem cell deficiency (LSCD). Methods:Sebacoyl chloride, dihydroxyl PCL and glycerol ethoxylate were used to synthesize PHFs.The thickness, transmittance and mechanical tensile properties of PHFs were measured.Four clean-grade New Zealand white rabbits were selected to culture primary limbal epithelial cells.The expression of keratin marker AE1/AE3 and stem cell marker p63 in the cultured cells were observed under a fluorescence microscope.The cells were divided into negative control group cultured with common cell culture solution, positive control group cultured with cell culture solution containing 100 μmol/L H 2O 2, and PHFs+ CECs group lined with PHFs cultured with common cell culture solution for 24 hours.The proliferation and apoptosis of cells in the three groups were observed by MTT and TUNEL staining, respectively.Fifteen clean-grade New Zealand white rabbits were divided into control group, PHFs group and PHFs+ CECs group by random number table method, with 5 rabbits in each group.LSCD model was constructed in the three groups.The control group was not given any treatment after modeling.In PHFs group, empty PHFs were placed on the corneal surface of rabbits.In PHFs+ CECs group, tissue-engineered grafts constructed with CECs after passage implanted on PHFs were placed on the corneal surface of rabbits.The corneal defect area of rabbits was detected and scored by fluorescein sodium staining.The histological characteristics of rabbits corneal epithelium was observed by hematoxylin-eosin staining.The use and care of animals complied with Guide for the Care and Use of Laboratory Animals by the U. S.National Research Council.The experimental protocol was approved by the Research and Clinical Trial Ethics Committee of The First Affiliated Hospital of Harbin Medical University (No.2021006). Results:The synthetic PHFs were with a thickness ≤150 μm, a tensile strength about 6 MPa, and a transmittance over than 99% in the range of 400-700 nm.Most of the cells from primary culture of limbal tissue were positive for AE1/AE3 and p63.MTT test results showed that the A490 value of PHFs+ CECs group, negative control group and positive control group was 0.59±0.01, 0.65±0.07 and 0.06±0.04, respectively, showing a statistically significant overall difference ( F=12.25, P<0.05). The A490 values of PHFs+ CECs group and negative control group were significantly higher than that of positive control group, and the differences were statistically significant (both at P<0.05). TUNEL test results showed that there was a significant difference in the TUNEL-positive cell rate among the three groups ( F=13.45, P<0.05), and the rates of TUNEL-positive cells in PHFs+ CECs group and negative control group were significantly lower than that in positive control group (both at P<0.05). Fluorescein sodium staining results showed that with the extension of postoperative period, the corneal fluorescein sodium staining score of the three groups decreased, which decreased successively in control group, PHFs group and PHFs+ CECs group.Hematoxylin-eosin staining showed fewer irregularly shaped corneal epithelial cells in the control group, and sparse single layer of corneal epithelial cells in some areas of the PHFs group.In PHFs+ CECs group, the corneal epithelium coverage was the largest, and the cell layers increased to 3-5, and the cells were with regular morphology and in close arrangement. Conclusions:PHFs have enough toughness, high transmittance and can expand corneal epithelium in vitro.PHFs are suitable for corneal epithelial transplantation and can promote the repair of corneal epithelium in rabbit model of LSCD.
6.Recent advance in TDP-43 proteinopathy in diagnosing and treating Alzheimer's disease
Hongfu JIN ; Hui SUN ; Shenrui GUO ; Yiyuan FENG ; Yafu YIN ; Hui WANG ; Weiwei CHENG
Chinese Journal of Neuromedicine 2023;22(9):942-946
The important role of TDP-43 proteinopathy in Alzheimer's disease (AD) has been gradually revealed. High proportion of AD patients have TDP-43 proteinopathy on their postmortem diagnosis. Patients with TDP-43 proteinopathy show more sever hippocampal atrophy and cognitive dysfunction, suggesting that TDP-43 proteinopathy can serve as an important target in AD diagnosis and treatment. Evaluation of TDP-43 proteinopathy in vivo would hold great promise in AD diagnosis, drug development and treatment. In this review, we describe the pathological characteristics of TDP-43 proteinopathy in AD, and summarize the recent progress of TDP-43 proteinopathy in the diagnosis and treatment of AD.
7.Clinical observation of adjusting middle urethral sling tension through anatomic position during RMUS
Jiayi LI ; Qixiang SONG ; Weilin FANG ; Yiyuan GU ; Yunyue GUO ; Wei ZHANG ; Lei XU ; Wei XUE
Chinese Journal of Urology 2022;43(9):675-680
Objective:To investigate the safety and effectiveness of tension adjustment technique using anatomical landmarks during retropubic midurethral synthetic sling.Methods:The data of 36 consecutive female patients with urinary incontinence, who had underwent retropubic midurethral synthetic sling procedure from January to August 2019 were analyzed retrospectively. The mean age was (60.83±7.93) years old and the body mass index was (24.43±2.44) kg/m 2. Among the recruited subjects, 36 had positive stress test and Marshall-Marchetti test. 20 (55.6%) were pure stress urinary incontinence, and 16 (44.4%) were mixed urinary incontinence. The severity of incontinence was classified into mild (5 cases, 13.9%), moderate (14 cases, 38.9%), severe (13 cases, 36.1%) and very severe (4 cases, 11.1%) using one-hour pad tests. Urodynamics were performed in 17 cases, with 5 (29.4%) presented detrusor overactivity, 3 (17.7%) possessed intrinsic sphincter deficiency. For each case, the tension of the sling was adjusted based on the anatomical landmarks, i. e. using an angled clamp attached closely to the pubic symphysis ventrally and the tip parallel to the edge of hymen dorsally. All patients were catheter-free right after the procedure. The subjective and objective effectiveness, and safety (the rate of urinary retension after surgery and postvoid residual volume 3 months later) were evaluated.The subjective cure rate was was defined as complete leakage free or very mild leakage during excessive bladder filling and fierce cough. The subjective effectiveness was defined as over 50% improvement of the leakage symptom. The objective cure rate was defined as a negative stress test. Results:For all 36 patients, the median hospital stays was 8 (5-95)h. No bladder perforation or transfusion cases. All patients were catheter-free right after the procedure, with no incidence of urinary retention. 27 patients completed a 3-month follow-up, with 22 had post-void residual data, 23 had subjective effectiveness data and 23 had objective effectiveness data. The median post-void residual was 7.5 (5-64) ml, subjective cure rate was 91.3% (21/23), and objective cure rate was 95.7% (22/23). 8.7% (2/23) reported difficult urination alleviated without the necessity of clinical interference. No urethra erosion or vagina extrusion was found. At 2-year follow-up, 34 patients completed assessment by phone. The subjective cure rate was 91.2% (31/34), with only 2.9% (1/34) reported difficult urination. Besides, at 3-month follow-up, there was no difference regarding the subjective cure rate [100.0%(12/12) vs. 81.8%(9/11)]or objective cure rate [91.7%(11/12) vs. 100.0%(11/11)] between patients with stress and mixed incontinence. No difference was noted among patients with mild, moderate, severe and very severe leakage[75.0% (3/4) vs. 100.0%(6/6) vs. 90.0%(9/10) vs. 100.0%(3/3)]. Of the 12 cases with urodynamic records, the presence of detrusor overactivity [66.7%(2/3) vs. 88.9%(8/9)] or intrinsic sphincter deficiency [0(0/1) vs. 90.9%(10/11)] did not significantly affected the cure rate of the procedure. At 2-year follow-up, there was no difference regarding the subjective cure rate between patients with stress and mixed incontinence [94.7%(18/19) vs. 86.7%(13/15)]. No difference was also noted among patients with mild, moderate, severe and very severe leakage[80.0%(4/5) vs. 100.0%(13/13) vs. 83.3%(10/12) vs. 100.0%(4/4)]. Of the 16 cases with urodynamic records, the presence of detrusor overactivity [60.0%(3/5) vs. 90.9%(10/11)]or intrinsic sphincter deficiency [66.7%(2/3) vs. 84.6%(11/13)]did not significantly affected the cure rate of the procedure.Conclusions:Tension adjustment using anatomic landmarks during sling procedure is safe and feasible for urinary incontinence, with minimum complications and residual volume, and high subjective/objective cure rate.
8.Program optimization before enucleation on ovine somatic cell nuclear transfer.
Yanhua GUO ; Yiyuan ZHANG ; Limin WANG ; Hong TANG ; Yingli LI ; Ping ZHOU
Chinese Journal of Biotechnology 2017;33(5):730-742
Ovine somatic cell nuclear transfer (SCNT) efficiency remains lower. Therefore, we optimized the program before oocyte enucleation on ovine SCNT. Four experiments were done including exposure duration of ovaries (3 h or 3 to 5 h), duration of oocytes maturation (18 h and 24 h), rate of donor adherent and enucleation time of maturate oocyte. The maturation rates of oocyte, fusion rates and cleavation rates of cloned embryos were used to assess the efficiency of different procedures. The maturation rates of ovaries with 3 h exposure was higher than that of 3 to 5 h (60.18% vs 52.50%) (P<0.05). Embryonic development competence had no significant difference (P>0.05). The maturation rates were significantly different between group18 h and 24 h (53.81% vs 89.06%, P<0.01). Embryonic development competence had no significant difference (P>0.05); fusion rates of donor adherent 30% group was higher than that of 10% group. Embryonic development competence had no significant difference (P>0.05). Different adherent donor characterizes the difference in plateau phase. The cleavation rates of 18 hpm group was higher than that of 16 hpm group. Embryonic development competence had no significant difference (P>0.05), the enucleation of 16 hpm group obtained one clone fetus, we got four clone fetus to repeat the 16 hpm group. Five microsatellite was analyzed by PAGE, the bands indicated that fingerprint of cloned fetus were completely the same as those of donor cells. Our data therefore suggests program optimization before enucleation assurance quality of material which be able to improve the quantity and quality of clone embryos, and optimized scheme can obtain clone sheep offspring.
9.Proteomics-based screening of differentially expressed proteins in skin of Chinese merino fine sheep (JunKen type) of different gender.
Yiyuan ZHANG ; Yanhua GUO ; Hong TANG ; Xinhua WANG ; Limin WANG ; Ping ZHOU
Chinese Journal of Biotechnology 2022;38(10):3925-3939
The purpose of this study was to understand the effect of gender on the traits of wool. One-year-old male and female Chinese merino sheep (JunKen type) were used to screen differentially expressed proteins in skin tissues by quantitative proteomics using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and data independent acquisition (DIA) strategy. This was followed by GO function annotation, KEGG metabolic pathway and protein network interaction analysis on the differential proteins obtained. The result showed that there were 674 differentially expressed proteins between male and female groups, whereas 280 proteins were up-regulated and 394 proteins were down-regulated in the male group. Through further comparison and analysis, there were 43 differentially expressed proteins related to skin hair follicle development and wool phenotype, 30 up-regulated and 13 down-regulated. The GO annotation analysis of differentially expressed proteins were mainly enriched in 37 processes of molecular function such as oxygen binding, chondroitin sulfate binding, heme binding, glutathione peroxidase activity and glutathione transferase activity; 120 biological processes such as cellular oxidant detoxification, regulation of muscle contraction, notch signaling pathway, calcium ion transmembrane transport and glutathione metabolism; and 31 processes related to cellular components such as mast cell granule, nucleus, sarcoplasmic reticulum and endoplasmic reticulum lumen. KEGG analysis showed that the differentially expressed proteins were involved sixteen signaling pathways, among which MAPK and p53 signaling pathways were closely related to wool growth and development. The protein network interaction analysis discovered that COL1A1 was closely related to MMP2, SPARC, THBS1 and other differentially expressed proteins, which might play a key role in wool growth and development. Thus, this study obtained basic data for revealing the molecular mechanism of sheep wool traits of different gender.
Animals
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Male
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Female
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Proteomics
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Chromatography, Liquid/methods*
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Tandem Mass Spectrometry/methods*
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Wool
;
China
10.Mixed urinary incontinence: will urgency and urge urinary incontinence symptoms resolve after RMUS?
Jiayi LI ; Wenxin XU ; Qixiang SONG ; Lei XU ; Yiyuan GU ; Yunyue GUO ; Jieying WANG ; Wei XUE
Journal of Modern Urology 2024;29(8):713-718
【Objective】 To explore the efficacy of retropubic midurethral synthetic sling (RMUS) in relieving urgency and urge urinary incontinence (UUI) symptoms in patients with mixed urinary incontinence (MUI). 【Methods】 Clinical data of 44 female MUI patients treated with RMUS during Jan.2018 and Dec.2020 in Shanghai Renji Hospital were retrospectively analyzed.All patients had positive results in stress test and Marshall-Marchetti test before operation, and 27 of them completed ICIQ-FLUTS-LF questionnaire.Urodynamic (UDS) tests suggested that 9 patients (20.5%) presented detrusor overactivity (DO).During RMUS procedure, the tension of the sling was adjusted based on the anatomical landmarks.The postoperative efficacy and improvement of urinary incontinence were analyzed. 【Results】 The patients aged (58.59±9.08) years, with a body mass index of 24.71±2.77.Among the 40 patients who completed telephone interview 2 years after surgery, the subjective cure rate was 85.0% (34/40).Among the 27 patients with records of questionnaires before and after surgery, there were significant differences in the incidence of urine leakage \[100% (27/27) vs. 18.5% (5/27)\], stress urinary incontinence (SUI) \[100% (27/27) vs. 18.5% (5/27)\] and UUI \[70.4% (19/27) vs. 29.6% (8/27)\] (P<0.05).However, no statistical differences were found regarding nocturia voiding episode (≥1 times), urgency, dysuria, hesitancy, strain to void, intermittent stream and enuresis (P>0.05).Based on preoperative UDS test, there were significant differences regarding the subjective cure rate in patients with or without preoperative DO \[55.6% (5/9) vs. 93.5% (29/31)\], incidence of SUI \[66.7%(4/6) vs. 4.8% (1/21)\], and UUI \[66.7% (4/6) vs. 19.0% (4/21)\] (P<0.05), while there was no statistical difference regarding urgency \[66.7% (4/6) vs. 33.3% (7/21)\] (P>0.05). 【Conclusion】 RMUS is effective in treating MUI patients with positive stress test and Marshall-Marchetti test results, which can relieve SUI and UUI symptoms, but has no effects on urgency symptoms.DO on preoperative urodynamics results in poorer subjective outcomes.