Objective To explore the effective mechanism of tumor necrosis factor-?(TNF-?) and interlukin-8(IL-8) in experimental lung damage by observing changes of TNF-? and IL-8 concentration in lung tissue during abdominal infection. Methods The cecal ligation and perforation were utilized to make the abdominal infection in rats.The rats were sacrificed respectively at 0,24,48,72,96 and 120 hours after operation. The pulmonary vascular permeability and the amount of different cells in bronchoalveolar lavage fluid(BALF) were examined. The concentrations of TNF-? and IL-8 in BALF, lung tissue and plasma were assayed. Results The pulmonary vascular permeability and the neutrophil percentage of BALF increased progressively. The concentrations of TNF-? and IL-8 significantly increased in plasma, lung tissue and BALF. Significant correlation was respectively present between the TNF-? levels and the IL-8 levels in lung and BALF. The levels of TNF-? and IL-8 in lung and BALF were not correlated respectively with those in plasma. Conclusions TNF-? and IL-8 may be implicated in the inflammatory reaction of lung during early stage of abdominal infection. Lung injury derived from intestinal infection could be early discovered through assaying TNF-? and IL-8 concentration in BALF.

BACKGROUND:With increased age,bone marrow mesenchymal stem cells(BMSCs)are influenced with regard to quantity and quality,which will induce great damages to the donors.Many studies have focused on seeking substitute MSC source.In contrast it remains controversial whether umbilical cord blood contains MSCs.OBJECTIVE:To isolate MSCs from human umbilical cord blood,and to detect their biological properties.METHODS:Umbilical cord blood samples were sterilely isolated using Percoll density gradient centrifugation to harvest intermediate layer cells.DMEM medium containing fetal bovine serum,penicillin,streptomycin and L-glutamine was added.Following several adherences and purification,the floating cells were discarded.Thus,many adherent cells with a confluence were collected.When cells were 60% 80% confluent,cells were digested by trypsin for subculture.Cells at passages,1,5 and 9 were obtained and their morphological changes were observed.Cell surface antigens were measured using flow cytometry.Growth curves were drawn,and cell viability was determined utilizing MTT.RESULTS AND CONCLUSION:Isolated umbilical cord blood MSCs presented an even size,showing spindle or star-shape fibroblasts-like cells.Umbilical cord blood MSCs at 1,5,9 passages were greatly positive for CD29,CD105 and CD166,but weakly positive for CD34 and CD45.Following 5 days of incubation,cells entered logarithmic growth phase.The number was decreased at day 9.Population doubling time was(53.5±8.32)hours.Cells grew well.Cells at 1-7 passages showed similar viability(P ＞ 0.05).Till passage 9,cell proliferation viability was decreased,but no significant difference was determined(P ＞0.05).Results verified that MSCs can be successfully isolated from umbilical cord blood in vitro.Cells at passages 1-9 presented a good reproductive activity.

Human upper extremity is the most complex and flexible executor during the human movement, coordination analysis of the synergetic control principle of human upper extremity is of great significance in trajectory planning and real-time control of anthropopathy robots and intelligent prosthesis system. Most studies have only been performed within the last 10 years. This paper surveys the research in the structure characteristic and redundancy coordination principle of human upper extremity, and the developments of various prospects of anthropopathy robots, intelligent prosthesis, gymnastic science and rehabilitation evaluation are discussed.
Humans ; Models, Theoretical ; Movement ; physiology ; Upper Extremity ; physiology

Objective To observe the effect of scalp-body acupuncture plus psychological intervention in treating post-stroke depression.Method Seventy-eight patients with post-stroke depression were divided into a treatment group (40 cases) and a control group (38 cases). In addition to ordinary neurological treatments, the treatment group also received scalp-body acupuncture plus psychological intervention while the control group also received Fluoxetine hydrochloride (Prozac). The interventions were given once a day, 4 weeks as a treatment course, for 2 courses in total. Hamilton Rating Scale for Depression (HAMD) and neurological impairment severity scale (NISS) were observed before intervention, after 1 treatment course and at the end of the intervention.Result After intervention, the HAMD and NISS scores dropped significantly in both groups, and the treatment group was markedly better than the control group (P＜0.05). The total effective rates of anti-depression and recovery of neurological function in the treatment group were significantly higher than those in the control group (P＜0.05).Conclusion Scalp-body acupuncture plus psychological intervention can produce a content efficacy in improving depression and recovering neurological function.

Aim To study the effect of tetramethylpyrazine(TMP) on binding of 125I-VEGF to VEGF receptor. Methods The mice sera were collected after peritoneal injection with big-dose TMP,low-dose TMP,protamine and NS. A reversed-phase high performance liquid chromatography(RP-HPLC) method was used to determine the TMP in mice serum. The culture medium of ECV304 was treated with the mice sera in different groups. Radioligand binding assay(RBA) of receptor and Scatchard pot were performed to observe the changes of the maximum binding capacity(B_ max) and dissociation constant(K_d).Results The sera of big-dose TMP inhibited 125I-VEGF binding to its receptor, K_d=343.30?36.64 pmol?L-1,B_ max=46.26?5.85 fmol/2?10~5 cells(P0.05),but B_ max decreased(P

Objective To observe the change of collagen in osteoarthritis and study the pathogenesis of osteoarthritis.Methods The right hind limb of twenty-four adult New Zealand White rabbits were immobilized with plaster cast in extension position for 10,20,30 and 40 days,respectively.Six animals without immobilization served as control.The articular cartilage of the medial femur condyle was harvested for transmission electron microscopy,in-situ hybridization of collagenⅡ,and immunohistochemistry of collagenⅠ,Ⅱ,Ⅲ.Results Transmission electron microscopy showed articular cartilage was destructed from the fine collagen fiber network of tangential zone.The fine collagen fiber network did not contain chondrocyte.Immunohistochemistry and in-situ hybridization showed that in earlier period of osteoarthritis,the collagen typeⅡand its gene expression firstly increased,then decreased with destruction of ultrastructure,and chondrocytes enhanced type Ⅱ collagen expressing and synthesizing mainly in transition zone and upper deep zone.In articular cartilage of osteoarthritis there was type Ⅲ collagen,instead of typeⅠcollagen.Conclusion In osteoarthritis,articular cartilage degenerated from tangential layer,in which collagen can not be repaired after destruction,this may contribute to the chondral degeneration.

Ligaments are the main parts which stabilize the knee joint. How to analog the ligaments in biomechanical model will affect the characteristics of the human knee dynamics and in the computation of the stress in ligaments between two bones. This symposium is aimed at the survey of the simplified method of the ligaments via mechanical parameters, and providing an exact method of constructing model.
Biomechanical Phenomena ; Humans ; Knee Joint ; anatomy & histology ; physiology ; Ligaments, Articular ; anatomy & histology ; physiology ; Models, Anatomic ; Models, Biological

Evaluation of human gait function is of great significance in clinical medicine and rehabilitation engineering. A quantitative gait evaluation method using principal component analysis was proposed. The evaluation steps included that a series of characteristic index was performed by the gait parameters with a gait detection, and the index was normalized, quantified and summarized by principal component analysis. Then the evaluation results were shown in formulation, figures and tables. The examples showed that this system could evaluate the recovery of the gait by treatment.
Evaluation Studies as Topic ; Gait ; physiology ; Humans ; Principal Component Analysis

A dynamics model of human lower extremity, which combines musculotendon dynamics and muscle excitation-contraction dynamics, is presented. With this model, a motion process of normal gait during swing phase is numerically analyzed by use of the optimal control theory. The model was verified using experimental kinematics, muscles activation, and electromyographic data. The result showed that the tri-phasic activation pattern and synergistic muscles displayed during a normal gait in swing phase. The pattern consists of three distinct phases, i. e., acceleration during moving initiation, braking the moving segment, and posture control at the final specified position.
Biomechanical Phenomena ; Computer Simulation ; Electromyography ; Gait ; physiology ; Humans ; Kinetics ; Lower Extremity ; physiology ; Models, Biological ; Muscle, Skeletal ; physiology ; Tendons ; physiology