Depression and insomnia are intimately related. Depressed patients usually manifest sleep discontinuity and early awakening, reduced or no slow wave sleep (SWS) and shortened latency of rapid eye movement (REM) sleep. These sleep abnormalities are very similar to those caused by over activated hypothalamic-pituitary-adrenal (HPA) axis with stress. Therefore, the animal models developed by post-traumatic stress disorder or chronic unpredictable mild stress could be used to evaluate drugs which have effects of both anti-depression and improvement of sleep quality, and to provide a more reliable platform for further studis on the mechanisms of depression and accompanied insomnia. This review mainly focuses on the typical features of sleep disturbance of depression, possible pathophysiological mechanisms, establishment of animal stress models and analysis of their abnormal sleep characteristics.

Objective To investigate the potential mechanism of inhibition of ursolic acid(UA) on growth of human gastric cancer cell lines SGC7901 in vitro.Methods SGC7901 cells were cultured in vitro,MTT assay was used to observe the effect of UA on growth of SGC7901 cells in various concentrations for different times.After SGC7901 cells were treated by 0—40 ?mol/L UA for 24 h,morphological changes were observed by inverted microscope.Apoptotic changes were detected by fluorescence microscopy and flow cytometry (FCM).Protein expressions of Bcl-2 and Bax were determined by Western blotting.Results UA(20—40 ?mol/L) could significantly inhibit the growth of SGC7901 cells in a(dose-and) time-dependent manner,the IC_(50) value of UA for SGC7901 cells for 12,24,36,and 48 h were((57.50?)1.18),(34.28?2.05),(27.54?1.11),and(24.83?1.02) ?mol/L,respectively.After UA((20—)40 ?mol/L) treatment for 24 h,SGC7901 cells turned round and floated at different levels;SGC7901 cells were arrested at G_0/G_1 phase and apoptosis was induced,and the apoptotic rate was increased along with the increase of UA concentration.Meanwhile Bcl-2 protein expression decreased,whereas Bax protein expression unchanged.Conclusion UA has a stronger antitumor effect on SGC7901 cells.Cytotoxic effect,proliferation inhibition,and apoptosis may be involoved in the mechanism of UA,and the apoptosis caused by UA may be enhanced by decrease of Bcl-2 protein expression.

Objective: To establish a Galleria mellonella model of liver abscess-related Klebsiella pneumoniae (K.pneumoniae) infection and to evaluate its feasibility for virulence detection. Methods: Twelve liver abscess-related K. pneumoniae strains were collected in Wuxi No.2 People′s Hospital from January 2016 to December 2017. Twenty K. pneumoniae strains isolated from sputum and urine samples were used as classic strains. All isolates were analyzed by String test. Common capsular serotypes (K1, K2, K5, K16, K20, K54 and K57) of highly virulent K. pneumoniae strains were detected by PCR. Virulence test was performed to measure 80% lethal doses (LD₈₀) of different serotypes in the same time period and the lethal time for 80% of larvae (LT₈₀) at the same concentration. Results: The 12 strains of liver abscess-related K. pneumoniae belonged to five serotypes, which were K1 (41.7%, 5/12), K2 (8.3%, 1/12), K5 (8.3%, 1/12), K20 (8.3%, 1/12) and K57 (33.4%, 4/12). High-virulence serotypes were not detected in the classic group. The positive rates of String test in the liver abscess group and the classic group were 75% and 10% (2/20), respectively. Results of the virulence test showed that when the concentration ranged from 1×10⁵ CFU/ml to 1×10⁸ CFU/ml, the lethal effects of different strains on Galleria mellonella larvae were in a concentration dependent manner. Twelve hours after infection, the numbers of dead larvae in K1 and K57 serotype groups were significantly higher than those in K2, K5, K20 and classic groups. The LD₈₀ values of the liver abscess group at 96 hours after infection were as follows: 1×10⁶ CFU/ml (K1, K57) and 1×10⁷ CFU/ml (K2, K5, K20). Conclusion The liver abscess-related K. pneumoniae isolates are all highly virulent strains. The virulence of K. pneumoniae can be detected at 12 hours after infection of Galleria mellonella.

Objective To investigate the distribution of hypervirulent capsular serotypes and virulence genes of Klebsiella pneumoniae associated with pyogenic liver abscess(PLA),and analyze the homology of strains.Methods Twelve strains of liver abscess-related Klebsiella pneumoniae were isolated in Wuxi Second People's Hospital during January 2016 and August 2017.Among them,five were also detected in blood samples.All the strains were performed the viscous thread test,and the hypervirulent capsular serotypes and main virulence genes were detected by PCR.The homology of the strains was analyzed by the multilocus sequence types(MLST) and pulsed-field gel electrophoresis(PFGE).Results The positive rate of the viscous thread test for 12 strains of liver abscess-related Klebsiella pneumoniae was 75％.Three kinds of hypervirulent capsular serotypes,including K1 (6/12,50％),K54(1/12,8.3％) and K57(5/12,41.7％),were detected.The positive rates of virulence genes,such as wcaG,rmpA,ureA,fimH,mrkD,uge,Aer and iroNB,were all 100％,while that of iucB was 83.3％.The cf29a gene was not found,and the magA,allS and kfuBC genes were only detected in K1 serotype strains.MLST found that ST23(4/12,33.3％) and ST25(3/12,25％) were main sequence types,and then were ST412(2/12,16.7％),ST1660(1/12,8.3％),ST1049(1/t2,8.3％) and ST11 (1/12,8.3％).PFGE showed that 12 strains of Klebsiella pneumoniae were divided into 8 types,and that only 3 strains of K1 serotype belong to the same clonotype.Conclusion All the isolated liver abscess-related Klebsiella pneumoniae strains are highly virulent.ST23 and ST25 are main sequence types,and ST1049 is first reported.PFGE results show genetic diversity,and Kl-type Klebsiella pneumoniae has a certain epidemic tendency.

Objective Explore the impact of long-term blood glucose fluctuations and blood glucose control on the stroke incidence in patients with type 2 diabetes mellitus(T2DM).Methods An observa-tional cohort of patients with T2DM was established based on"Shanghai Community Chronic Disease Health Management Service Objects"on October 1st,2018.Follow-ups were conducted every three months,and fasting blood glucose(FBG)were tested at each visit.Basic epidemiological data were collected via the Shanghai Community Health Management Information Platform or survey questionnaires,and stroke incidents were gathered via the"Shanghai Cardio-Cerebrovascular Event Monitoring System".The first reported stroke incident within the observation period was considered.The observational deadline was December 31st,2021.Standard deviation of FBG was used to evaluate blood glucose fluctuation and FBG control rate was used to reflect blood glucose control status.Cox Proportional Hazards Model was utilized to analyze the impacts.Results The cumulative observation time was 91,826.1 person-years for the study,in which there were 1785 cases of stroke events observed,implying a cumulative incidence of 5.73%and incidence rate of 1943.9/100000 person-years.The mean number of follow-ups(10.29±3.07)per patient was recorded.The details of the stroke cases were as follows:transient ischemic attacks(n=111,6.22%),non-lacunar brain infarctions(n=754,42.24%),lacunar brain infarctions(n=798,44.71%),intracerebral hemorrhages(n=80,4.48%),non-intracerebral hemorrhages(n=8,0.45%),subarachnoid hemorrhages(n=12,0.67%),and unclassified strokes(n=22,1.23%).After excluding subjects with less than five follow-ups,the Cox Proportional Hazards Model suggested that increased standard deviation of FBG was an independent risk factor for the first stroke incident,and an increased blood glucose control rate was an independent protective factor.Both the standard deviation of FBG and the blood glucose control rate were not correlated with stroke recurrence.Conclusion Long-term blood glucose fluctuations and blood glucose control can predict the risk of a first stroke in patients with T2DM.The relationship between blood glucose fluctuations,blood glucose management,and stroke recurrence requires further studies.

Objective To explore the effect of jogging on the patients with depression disorder of liver-stagnation and spleen-deficiency.Methods 100 patients with liver-stagnation and spleen-deficiency depression disorder were randomly selected from Tongde Hospital of Zhejiang Province from March to September 2022.They were divided into observation group and control group(50 cases each)by random number table method.The control group received conventional antidepressant drug treatment,and the observation group was given jogging exercise regimen on the basis of the control group:exercise at least 4 times a week,30-60 min each time,and the heart rate was maintained at 110-120 times/min for 4 weeks.The depressive disorder of the patients was assessed by Zung's Self-rating Depression Scale(SDS)and 17-item Hamilton Depression Scale(HAMD-17).Results After jogging exercise intervention,the total effective rate of the observation group was 94%,which was significantly higher than that of the control group 80%(P<0.05).HAMD-17 score was significantly lower in the observation group than in the control group after intervention(F=12.575,P<0.05).The scores of cognitive impairment,block and sleep disturbance in the observation group were significantly lower than those in the control group after intervention(F=12.575,P<0.05).The body weight factor scores of the observation group and the control group were significantly higher after intervention than before intervention(P>0.05).Conclusions Jogging exercise intervention therapy can significantly improve the depression of patients with liver-qi and spleen-deficiency depression disorder,and jogging can be an effective method to prevent and treat depression disorder.