Objective:To screen the potential characteristic gene spectrums and signal pathways of osteoarthritis based on gene chips.Methods:We analyzed 2 microarrays of human joint synovial tissue (GSE82107 and GSE55235) derived from the Gene Expression Omnibus (GEO) database and included for this study 20 osteoarthritis (OA) samples and 17 healthy control samples. The differentially expressed genes (DEGs) between OA and HC were screened by GEO2R tool. Analyses of Gene Ontology function and Kyoto Encyclopedia of Genes and Genomes pathway enrichment were performed using the Database for Annotation, Visualization and Integrated Discovery to identify the pathways and functional annotations of DEGs (https://david.ncifcrf.gov/). Protein-protein interaction of these DEGs was analyzed based on the Search Tool for the Retrieval of Interacting Genes database and visualized by Cytoscape software (http://www.string-db.org/).Results:191 up-regulated DEGs and 49 down-regulated DEGs were screened out from the 2 microarray databases. Enrichment of DEGs was mainly found in regulation of such biological functions as "inflammation" , "bone cell differentiation" and "positive apoptotic cell regulation" , HTLV-I infection, silk crack on the original amp-activated protein kinase (MAPK) signaling pathway, swine flu, tumor necrosis factor (TNF) signaling pathway, the nf-kappa B signaling pathway, PI3 kinase/Akt pathway, toll-like receptor pathway, legionella, salmonella and other 14 signaling pathways. In 2 modes of MNC and Degree, the top 10 core genes were screened, of which interleukin-6 (IL6), JUN, chemokine 8 (CXCL8), early reaction growth factor (EGR1) and cyclin (CCND1) were identified as valuable biomarkers of OA.Conclusions:Based on GEO chips, 10 characteristic gene profiles such as IL6, JUN, CXCL8, EGR1, CCND and 14 signal pathways such as tumor necrosis factor (TNF) signal pathway, NF-κB signal pathway, PI3 kinase/Akt pathway and Toll-like receptor pathway were screened, which may provide new clues for understanding of the pathogenesis of osteoarthritis.

Systemic lupus erythematosus(SLE)is characterized by a large number of anti-autoantibodies produced by abnormally activated B cells,which form immune complexes with autoantigens to induce systemic inflammation,leading to the involvement of multiple systems and organs.Existing treatment strategies targeting B cells(such as belimumab,telitacicept,and rituximab)had limited efficacy.Recently,CD 19 targeting chimeric antigen receptor(CAR)-T cell has shown excellent efficacy in the treatment of SLE.However,autologous CAR-T cell therapy has potential risks such as cytokine release syndrome(CRS),T cell tumors,and infections,and it is expensive.CAR-natural killer(NK)cell therapy,on the other hand,is an off-the-shelf cellular therapy with high safety,no neurotoxicity or tumorigenic risk,and with low treatment costs.Currently,CAR-NK cell has achieved remarkable research results in treating hematological malignancies,and preliminary clinical studies of CAR-NK cell therapy for SLE have showed good efficacy,excellent safety,and predictable durability.This article focuses on the characteristics of the new generation of universal CAR-NK cell and the latest clinical research results for the treatment of relapsed and refractory SLE,offering insights into its promising future in the treatment of SLE and B-cell-related autoimmune diseases.

Objective lo investigate the recovery of urinary function and related influencing factors in patients with stress urinary incontinence(SUI)after the tension-free vaginal tape procedure at the mid-urethra.Methods A total of 112 SUI patients who underwent the tension-free vaginal tape procedure at the mid-urethra in Beijing Rehabilitation Hospital Affiliated to Capital Medical University from October 2018 to October 2023 were selected as the research subjects.The postoperative urinary function recovery of all patients was recorded.According to the postoperative urinary function recovery time,patients with a recovery time of≤3 days were included in the rapid recovery group,and those with a recovery time of＞3 days were included in the non-rapid recovery group.Clinical data including age,body mass index,menopausal status,vaginal delivery history,number of deliveries,disease severity,comorbid hypertension,diabetes,history of pelvic organ prolapse,maximum urinary flow rate,postoperative urinary retention,preoperative bladder residual urine volume,maximum bladder volume,hemoglobin(Hb)level,white blood cell(WBC)count,red blood cell(RBC)count,and platelet(PLT)count of patients between the two groups were compared.Factors affecting the recovery of urinary function in SUI patients were analyzed by multivariate logistic regression.Results The recovery time of urinary function in 112 patients with SUI ranged from 1 to 15(4.10±0.78)days.Specifically,64 patients(57.14％)recovered within 3 days,and 48 patients(42.86％)recovered in more than 3 days.The proportions of patients with menopause,history of vaginal delivery,history of pelvic organ prolapse,and postoperative urinary retention in the non-rapid recovery group were significantly higher than those in the rapid recovery group,and the maximum urinary flow rate was significantly lower than that in the rapid recovery group(P＜0.01).There were no significant differences in age,body mass index,number of deliveries,preoperative bladder residual urine volume,maximum bladder volume,Hb level,WBC count,RBC count,PLT count,disease severity,proportion of patients with comorbid hypertension and proportion of patients with diabetes between the two groups(P＞0.05).Multivariate logistic regression analysis showed that menopause,history of vaginal delivery,history of pelvic organ prolapse,postoperative urinary retention and maximum urinary flow rate were influencing factors for the recovery of urinary function in SUI patients after surgery(P＜0.05).Conclusion The recovery of urinary function in SUI patients after a transvaginal tension-free vaginal tape procedure at mid-urethra is not ideal.Menopause,history of vaginal delivery,history of pelvic organ prolapse,postoperative urinary retention and maximum urinary flow rate are the influencing factors for the recovery of urinary function in SUI patients.