Objective:To investigate the expression of miRNA‐146a in the inflammatory response model of rat Schwann cells (SCs) cultured in vitro .Methods:Lipopolysaccharide(LPS) was applied to induce inflammatory response model of rat SCs .The cyclooxygenase‐2(COX‐2) inhibitor (NS398) was added six hours after LPS had been used .In normal group ,the same amount of culture medium was replaced while the LPS was applied in LPS group .The expression of miRNA‐146a and the proliferative capacity of SCs were detected by real‐time PCR and CCK‐8 method .Results:Compared with that in the normal group ,the ex‐pression levels of miRNA‐146a in all LPS groups increased significantly (P<0 .05) ,and the expression level of miRNA‐146a was up‐regulated in accordance with the increase of the concentration of LPS .If NS398 was added six hours after the applica‐tion of 1 .0 μg/mL LPS ,The expression of miRNA‐146a was down‐regulated .Six hours after the application of 10 μg/mL LPS ,the proliferative capacity of SCs declined .However ,if NS398 was added six hours after the application of LPS ,then the proliferative capacity of SCs improved .Conclusions:The miRNA‐146a is involved in the regulation of inflammatory response in Schwann cells cultured in vitro ,and its expression level may reflect the severity of inflammatory response to a certain extent .

Animals ; Cell Differentiation ; Hepatocyte Growth Factor ; genetics ; metabolism ; Membrane Proteins ; genetics ; metabolism ; Mice ; Mouse Embryonic Stem Cells ; cytology ; metabolism ; Protein Precursors ; genetics ; metabolism ; Serine Endopeptidases ; genetics ; metabolism