1.Effect of somatostain intra-articular injection on metabolism of free radicals in rabbits with knee osteoarthritis
Zhentao ZHANG ; Yixuan WANG ; Zhaohua LIU
International Journal of Surgery 2011;38(10):684-687
Objective To discuss the effect of somatostain SS on metabolism of free radicals in animal models of osteoarthritis,so to discuss the treatment mechanism of intra- articular injection of somatostatin on osteoarthritis.MethodsForty experimental Japanese big-ear rabbits were randomly divided into four groups:normal control group,blank model group,normal saline group and SS group,n =10 for each group.After modeling,the rats in SS group and normal saline group received intra- articular injection of somatostain and saline respectively on the right knee.Ten weeks after the modeling,we measured the movement of right knee joint in rabbits,the levels of nitric oxide ( NO ) and hyaluronic acid (HA) in serum from the heart blood,and the activity of SOD and the content of MDA in synovial tissue.Results Compared with the blank model group,the movement of the knee joint was significantly increased in SS group,(96.01 ± 1.06)vs (50.21 ± 1.80) (P < 0.01 ).In the blank model group,the levels of NO and HA (μmoL/mL) in serum were ( 111.60 ± 2.76) and ( 309.11 ± 1.89 ),which was significantly decreased in SS group,which was( 80.14 ± 1.67 ) and ( 133.73 ± 2.75 ) ( P1 =0.0049,P2 =0.0052,P < 0.01 ).Compared with the blank model group,the SOD activity was (15.77 ± 2.76) and the MDA in serum nmol/mg prot was( 1.33 ±1.03),while was significantly increased in SS group,(24.74 ± 1.67) ( P < 0.01 ),and the levels of MDA in serum was significantly decreased in the SS group,(0.89 ±1.46) (P<0.01).Conclusion Intra-articular injection of somatostatin in knee osteoarthritis can improve the oxidative stress,enhance the activity of the knee joint.
2.Advances in Study on Interaction between Gut Microbiota and Brain-gut Axis
Lu ZHANG ; Yixuan LIU ; Liping DUAN
Chinese Journal of Gastroenterology 2014;(9):563-565
Gut microbiota plays an important role in the maintenance of physiological function and genesis of some gut diseases. Brain-gut axis,as an important link between brain and gastrointestinal tract,is a requisite of gut microbiota stability. The dysfunction of brain-gut axis may lead to intestinal dysbiosis through activation of intestinal immune activity. Conversely,intestinal dysbiosis can influence nervous system development and may cause dysfunction of brain-gut axis,in which vagus nerve and metabolites in serum may be the critical factors. This article reviewed the advances in study on interaction between gut microbiota and brain-gut axis.
3.Content Determination of Astragaloside Ⅳ in Qizhi Jiangtang Granules by HPLC-ELSD
Qiuyue LI ; Yixuan ZHANG ; Yue SHI
China Pharmacy 2007;0(32):-
OBJECTIVE:To establish HPLC-ELSD method for content determination of astragaloside Ⅳ in Qizhi jiangtang granules. METHODS:The separation was performed on Hypersil ODS2(250 mm?4.6 mm,5 ?m)column with acetonitrile-water(32 ∶ 68)as mobile phase. The flow rate was 1.0 mL?min-1. Injection volume was 10 ?L. The column temperature was 25 ℃. The temperature of drift tube for measurement was 110 ℃ and flow rate of N2 was 3.12 SLPM. RESULTS:Regression equation was A=1.699 C+6.181. The linear range of astragaloside Ⅳ was 0.259~1.295 mg?mL-1(r=0.999 6,n=5) with lowest detectable limit of 5.18 ?g?mL-1. An average recovery rate were 97.75%(RSD=2.17%). CONCLUSION:The method is simple,rapid and reliable for quality control of Qizhi jiangtang granules.
4.Pregnancy outcome of frozen-thawed embryo transfers in different endometrial progesterone preparation time
Jin LU ; Cuilian ZHANG ; Shaodi ZHANG ; Jiaxuan GENG ; Yixuan ZHANG
Chinese Journal of Obstetrics and Gynecology 2014;49(6):424-427
Objective To investigate the pregnancy outcome of frozen-thawed embryos transfer in different endometrial progesterone preparation time.Methods From January to December 2012,pregnant outcome of 1 103 frozen-thawed embryo transfer cycles using artificial periodic endometrial preparation were studied retrospectively in Reproductive Medical Center of Henan Provincial People's Hospital.It was divided into 4 groups:group 3-3 (n =543,3 days after endometrial progesterone preparation and transfer D3 embryo),group 4-3(n =330,4 days after endometrial progesterone preparation and transfer D3 embryo),group 5-5 (n =150,5 days after endometrial progesterone preparation and transfer D5 blastula),group 6-5 (n =80,6 days after endometrial progesterone preparation and transfer D5 blastula).The rate of implantation,pregnancy,ectopic pregnancy,multiple pregnancy,and first trimester abortion were compared among those groups.Results (1) With the different endometrial progesterone preparation methods and transfer D3 embryos,implantation rate [group 3-3:39.9% (429/1 074) ; group 4-3:44.1% (286/648)],pregnancy rate [group 3-3:56.0% (304/543) ; group 4-3:59.4% (196/330)],ectopic pregnancy rate [group 3-3:3.3% (10/304) ; group 4-3:2.6% (5/196)],multiple pregnancy rate [group 3-3:38.5% (117/304) ; group 4-3:43.4% (85/196)]and early abortion rate[group 3-3:13.5% (41/304); group 4-3:13.3% (26/196)] had no significant differences between group 3-3 and group 4-3 (all P > 0.05).(2) With the different endometrial progesterone preparation methods and transfer D5 blastocysts,implantation rate [group 5-5:64.7% (191/295) ; group 6-5:69.4% (100/144)],pregnancy rate [group 5-5:80.7% (121/150) ; group 6-5:78.8% (63/80)],ectopic pregnancy rate [group 5-5:2.5% (3/121) ; group 6-5:0],multiple pregnancy rate[group 5-5:55.4% (67/121) ; group 6-5:46.3% (37/80)] and early abortion rate[group 5-5:5.8% (7/121); group 6-5:7.9% (5/63)].However,there were no significantly differences between group 5-5 and group 6-5 (all P > 0.05).Conclusions The two different progesterone transformed endometrial methods can obtain satisfactory clinical outcome with D3 embryo or D5 blastocysts transfor.It is convenient to clinical and laboratory work arrangements.
5.Effect of inherent depression on chronic visceral hypersensitivity induced by colon acetate stimulation in neonatal rats
Yixuan LIU ; Yongshen ZHANG ; Liping DUAN ; Lu ZHANG ; Changqing YANG
Journal of Peking University(Health Sciences) 2015;(2):289-294
Objective:To explore the effect of inherent depression on chronic visceral hypersensitivity. The differences of visceral sensitivity, colitis, and brain activation between Fawn-Hooded ( FH/Wjd) and Sprague-Dawley( SD) rats were identified after neonatal colon acetate stimulation.Methods:The specific pathogen free Fawn-Hooded (FH/Wjd) and Sprague-Dawley(SD) rats were used to establish irritable bowel syndrome (IBS) model.The visceral sensitivity was measured by colorectal distension (CRD). The expression of 5-hydroxytryptamine (5-HT), mast cell (MC), indoleamine 2,3-dioxygenase (IDO) in colon and IDO in specific cerebral regions were detected through immunohistochemistry.Results:Ab-dominal withdrawal reflex ( AWR) scores showed that visceral sensitivity of acetate-enema groups was sig-nificantly higher than that of saline-enema groups ( FH/Wjd:2.44 ±0.04 vs.1.96 ±0.07, P<0.05;SD:1.75 ±0.13 vs.1.32 ±0.05, P<0.05).Furthermore, FH/Wjd rats of IBS group scored signifi-cantly higher than SD rats of IBS group (2.44 ±0.04 vs.1.75 ±0.13, P<0.05).The MC amounts of both SD and FH/Wjd IBS group rats were significantly more than those of their control groups ( FH/Wjd:43.24 ±1.72 vs.24.92 ±1.38, P <0.01.SD:23.80 ±1.28 vs.14.24 ±0.92, P <0.01).
Besides, the MC amounts of control and IBS group of FH/Wjd rats were significantly more than that of SD IBS group rats ( P<0 .01 ) .The IDO and 5-HT positive cells in colonic mucosa of IBS group of both SD and FH/Wjd rats were significantly more than those of their control groups, respectively(P <0.01). The IDO, 5-HT positive cells in colonic mucosa of both control and IBS group of FH/Wjd rats were significantly more than those of both control and IBS group of SD rats ( control:IDO,24.64 ±2.22 vs. 15.52 ±1.39;5-HT,21.32 ±1.26 vs.12.72 ±1.12.IBS: IDO,44.92 ±2.31 vs.20.85 ±1.72;5-HT, 31.84 ±1.57 vs.19.65 ±1.09.P <0.01).The expression of IDO in prelimbic cortex (PrL) areas of FH/Wjd IBS rats was significantly higher than that of IBS group of SD rats (49.60 ±4.31 vs. 35.60 ±2.42, P <0.01) , and the expression of IDO in rostral anterior cingulate cortex ( rACC) areas of FH/Wjd IBS rats was significantly more than that of FH/Wjd control rats (45.44 ±1.16 vs.34.08 ± 2.76, P <0.01) .Conclusion:Inherent depressive FH/Wjd rats were more sensitive to neonatal colon acetate stimulation, presenting as visceral hypersensitivity which maybe associated with increased MC amounts and over-expression of 5-HT and IDO in colon, suggesting that depression disorder may aggra-vate functional disturbance of gastrointestinal tract by regulating the response to inflammatory stimulation.
6.Short and long term clinical outcome of 283 cases of nasopharyngeal carcinoma patients treated with hy-perfractionated radiotherapy
Zhiyuan XU ; Hanxing LIN ; Xiaoping ZHANG ; Yixuan LI
Journal of International Oncology 2008;35(9):711-713
Objective To approach the therapeutic effects and complication of hyperfractionated radio-therapy for nasopharyngeal carcinoma. Methods From January 2000 to November 2002,283 patients with na-sopharyngeal carcinoma were treated with hyperfraetionated radiotherapy. For hyperfractionated radiotherapy,γ-ray from 60Co was performed and two fractions of 1.2 Gy were given daily,with an interval of≥6 h,5 days per week to a median dose of 72 Gy for nasopharynx and 68.4 Gy for neck. For some patients, carboplatin or cispla-tin and 5-fluorouracil were transfused. Results The incidence rate of≥3 grade acute mueositis was 12. 4%.The complete remission rate after treatment was 93.6%. The 1,2 and 5 year survival rate was 93.6% ,82. 7%and 60. 1% respectively. Conclusion patients treated with hyperfractionated radiotherapy have good tolerance and therapeutic effect. It provids a treatment means for nasopharyngeal carcinoma. But it must be validated by the large-scale clinical trials.
7.Construction, purification and substrate specificity identification of recombinant human platelet-activating factor acetylhydrolase isoformⅠ
Xiaoying CHEN ; Jing XU ; Junwei YANG ; Yixuan ZHANG
Journal of Shanghai Jiaotong University(Medical Science) 2009;29(10):1152-1156
Objective To construct and purify the recombinant protein of platelet-activating factor acetylhydrolase (PAF-AH) isoform I , and study the enzyme activity by different substrates. Methods The (3 subunit of PAF-AH isoform I was cloned and expressed in E. coli. Exogenously expressed recombinant protein was purified to SDS-PAGE homogeneity, and its activity was identified by arylesterase detection. Phenylacetate, 1-O-hexadecyl-2-deoxy-2-thioacetyl-sn-glycero-3-phosphocholine ( 2-Thio PAF) and l-myristoy1-2-( 4-nitrophenylsuccinyl) phosphatidylcholine (the latter two were commercial plasma PAF-AH substrates) were used for the substrate identification. The plasma type PAF-AH was served as positive control. Results Recombinant protein of β subunit of PAF-AH isoform I was successfully constructed and expressed in E. coli after purification. Compared with positive control, the recombinant protein could hydrolyze phenylacetate and 2-Thio PAF, but could not hydrolyze l-myristoyl-2-( 4-nitrophenylsuccinyl) phosphatidylcholine. Conclusion Recombinant protein of β subunit of PAF-AH isoform I can be successfully constructed. There are differences in the substrate specification to the two commercial PAF substrates for PAF-AH isoform I and plasma type PAF-AH, which provides a quick method to differentiate PAF-AH isoform I from plasma type PAF-AH.
8.Overexpression of connexin 43 in bone marrow mesenchymal stem cells in Xiao Meishan swines.
Yixuan FAN ; Yanli ZHANG ; Qiang WANG ; Caifang REN ; Feng WANG
Chinese Journal of Biotechnology 2015;31(3):351-360
We studied the function of connexin 43 (Cx43) gene in Xiao Meishan swine bone marrow mesenchymal stem cells (BMSCs) resulting from overexpression. Cx43 eukaryotic expression vector (pEGFP-Cx43) was constructed and transfected into BMSCs by nucleofector, after detecting the transfection efficiency; the expression of Cx43 was verified by RT-PCR, immunofluorescence and western blotting. Furthermore, we detected its cell cycle and apoptosis through flow cytometry. Our results show that pEGFP-Cx43 plasmid was successfully constructed, and green fluorescence in pEGFP-Cx43 transfected BMSCs was highly expressed with 60% transfection efficiency. In transgenic Xiao Meishan swines BMSCs, the expression level of Cx43 mRNA and protein were up-regulated. Meanwhile, the ability of cell proliferation was significantly increased, and the apoptosis rate was significantly reduced. Taken together, Cx43 overexpression could promote the proliferation of Xiao Meishan swine's BMSCs and markedly reduce their apoptosis, which provides evidence for in vivo research.
Animals
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Animals, Genetically Modified
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Apoptosis
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Cell Proliferation
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Connexin 43
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metabolism
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Flow Cytometry
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Genetic Vectors
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Mesenchymal Stromal Cells
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metabolism
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Plasmids
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Swine
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genetics
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Transfection
9.Survey on the Current Status of Job Satisfaction of Clinical Medical Staff and Research on Coping Strategies
Xiaoxiao ZHANG ; Yixuan ZHU ; Jingtong WANG ; Shan WANG
Chinese Journal of Medical Science Research Management 2015;28(3):260-265,282
Objective To explore the current status of job satisfaction of clinical medical staff and factors that influence the job satisfaction,and to put forward coping strategies and suggestions for improving the job satisfaction of clinical medical staff,so as to maintain workforce stability.Methods The paper will investigate the job satisfaction of clinical medical staff by sending questionnaires to all doctors and nurses working in a hospital,and use descriptive analysis and T test statistic method to analyze the current status of job satisfaction of questionnaire receivers as well as different dimensions of job satisfaction among different population groups.Results More than 85% of questionnaire receivers think that the job they are doing is at higher risk.Over 80% feel stressed out by their jobs.Less than 35% are satisfied with their occupations,social status,working status,job security,and promotion opportunities.Less than 15% feel respected by their jobs.Conclusions Generally,questionnaire receivers' job satisfaction is low.Heavy workload and poor quality of life are leading factors that contribute to their low job satisfaction.In this sense,we should adopt various measures to give attention to these key population groups.
10.Analysis on ADR Case Reports of Our Hospital in 2008
Shiyong ZHANG ; Jun CHENG ; Ruqiang WANG ; Yixuan HAN
China Pharmacy 2007;0(26):-
OBJECTIVE:To probe into the characteristics and regularity of adverse drug reactions(ADR)cases occurred in our hospital.METHODS:535 ADR cases reported in our hospital in 2008 were collected and analyzed statistically.RESULTS:In the incidence of 535 ADR cases,the ratio of male to female were 1.10∶1.Patients over the age of 60 years showed the highest incidence,accounted for 36.82%(n=197).430 ADR cases were induced by intravenous route(80.37%);254 cases were induced by antimicrobials(47.48%),followed by TCM preparations(n=75,14.02%).The ADR were chiefly manifested as lesion of skin and its appendants in 212 cases(39.63%),followed by 164 cases of lesion of digestive system(30.65%).Most ADR cases had a favorable prognosis.CONCLUSION:It should be strengthened to offer education and guidance of ADR monitoring for medical staff so as to make sure the safety,effectiveness and convenience of drug use in the clinic.