This experiment was designed to investigate the expression profile of miRNAs (microRNA) in human gastric cancer tissues.Methods The expression profiles of miRNAs were compared between 3 pairs of GC and adjacent normal tissues using an Exiqon miRNA array,following which quantitative PCR (qPCR) was employed to confirm the results of the miRNA array,and 10 miRNAs were selected.Bioinformatics was used to analyze the biological function of the differentially expressed miRNAs and its target genes in gastric cancer.Results Compared with adjacent mucosal tissues,95 miRNAs were up-regulated and 16 miRNAs were down-regulated in GC (＞ 2 folds,P ＜ 0.05).The qPCR results were consistent with microarray-based expression analysis (P =0.049).Furthermore,the online GO and pathway analysis revealed that miRNAs might involve RNA transcription,RNA metabolism,gene expression,gene silencing,and other biological functions in GC.Conclusion There is abnormal expression of miRNAs in gastric cancer,and the abnormal expression of miRNAs may be related to GC tumorigenesis.

Objective To describe common dermoscopic features of primary cutaneous amyloidosis (PCA),and to explore the application value of dermoscopy in assisting the diagnosis of PCA.Methods Seventeen patients with PCA were collected from Department of Dermatology of Peking Union Medical College Hospital between April 2014 and December 2016.A total of 45 skin lesions were subjected to dermoscopy,and their dermoscopic features were analyzed.Results The common dermoscopic features of PCA included central hubs,which could be white,brown or scar-like areas,various pigment structures and shiny white streaks.Dermoscopy showed that central hubs could be observed in all the lesions (100％),while white center hubs alone were observed in 14 (31％) lichenoid lesions,both white center hubs and scar -like areas in 5 (11％) lichenoid lesions,brown center hubs alone in 8 (18％) macular lesions,both white and brown center hubs in 6 (13％) lichenoid lesions and 17 (38％) macular lesions.All the lesions showed various pigment structures.Shiny white streaks were observed in 4 (9％) lichenoid lesions.Conclusion Dermoscopy has good application value in assisting the diagnosis of PCA.

Objective To detect the impact of Diltiazem on the major adverse cardiac events (MACE) in six months after percutaneous coronary intervention (PCI).Methods A total of 192 patients after PCI with coronary atherosclerotic heart disease were enrolled in this study.The patients were randomly divided into Diltiazem therapy group (101 patients) and non-Diltiazem therapy group (91 patients).The high-sensitivity C-reactive protein (hs-CRP) was assessed before and 24 h after PCI,and the incidence of Major adverse cardiovascular events(MACEs) were assessed at the sixth month after PCI.Results Compared with before PCI,hs-CRP level increased significantly in both group after PCI (P＜0.01),but hs-CRP level was lower in Diltiazem therapy group than in non-Diltiazem therapy group (P＜0.05).Compared with non-Diltiazem therapy group,there was lower incidence of MACEs during six months follow-up in Diltiazem therapy group.Conclusions Diltiazem can decrease the incidence of MACEs during six months after PCI.

Objective To investigate the curative effect of auditory integration training (AIT) on autism spectrum disorders (ASD) in children with abnormal brainstem auditory evoked potential (BAEP).Methods 56 cases of ASD patients with abnormal BAEP were treated with AIT (aged 2-6 years).BAEP will be reviewed after each course of treatments until the test results were back to normal or had no obvious changes.Children' s core symptoms were evaluated by using autism behavior checklist (ABC) and the childhood autism rating scale (CARS) before and after treatments.Results 56 children accepted (1.95±0.92) courses of AIT.Compared with the data before AIT,CARS scores ((36.32± 3.54),(34.11 ± 3.12)),scores of the sensory factor((5.65±4.61),(4.28±4.11)) and the stereotypes factor of ABC were decreased significantly(P＜0.05).After treatments,BAEP of 29 cases (51.79％) went back to normal levels.The bilateral wave incubation periods on left side (Ⅰ:(1.81 ± 0.17) ms,(1.71 ± 0.12) ms,Ⅲ:(4.14 ± 0.18) ms,(4.07 ±0.17)ms,V:(6.09±0.23)ms,(5.97±0.22) ms)and right side (Ⅰ:(1.79±0.17) ms,(1.74±0.13) ms,Ⅲ:(4.15±0.16) ms,(4.07±0.16) ms,V:(6.06±0.23) ms,(5.99±0.26) ms) were significantly shortened (P＜ 0.05).Conclusion AIT can improve the functional handicap of auditory pathway in brainstem of ASD children,and the core symptoms of ASD.

Objective To study the effect of RNA interference on the expression of CTGF in skin fibroblasts of systemic sclerosis(SS). Methods Four CTGF specific siRNAs and a negative control siRNA were designed and then synthesized by in vitro transcription. siRNAs labeled with carboxyfluorescein-6-succimidyl ester (FAM) were transiently transfected into SS skin fibroblasts. Forty-eight hours after the fibroblasts were treated with siRNAs, the mRNA and protein expression of CTGF was detected by semiquantitative RT-PCR and Western blot analysis, respectively. Results The mRNA and protein expression of CTGF in fibroblasts was significantly down-regulated by 4 and 3 CTGF specific siRNAs (both P

Volume management is recognized as an important determinant of dialysis adequacy. The optimal ultrafiltration and sodium removal will improve the volume management and reduce the cardiovascular mortality. Automated peritoneal dialysis (APD), as one of renal replacement therapies, has become a choice for more and more patients with end-stage renal disease. APD allows an individualized dialysis prescription by providing more dialysis doses and more exchange times to improve ultrafiltration and sodium removal, achieving or even exceeding the efficacy of continuous ambulatory peritoneal dialysis. New strategies for volume control have emerged, including adapted APD, using icodextrin and low-sodium dialysate, to provide new ideas for APD prescription adjustment.

Objective To explore the application effects of Omaha problem classification system on clinical follow-up in patients with peritoneal dialysis (PD) at home. Methods 60 outpatients with PD at home were selected into the study from May to September 2016. Based on Omaha problem classification system, problems referred to physiology,social psychology,health-related behavior and environmental issues of nursing care were assessed. Results A total of 436 nursing problems were found,with about 7.27 problems per patient on average. Approximately 65.6% problems for physiology,23.2% for health-related behavior,7.8% for environmental issues,and 3.4% for social psychology. The most common nursing problems referred to circulation (90.0%),pain (80.0%),skin (71.7%),and digestion (63.3%). Conclusions Omaha problem classification system can comprehensively assess nursing problems in outpatients with PD at home. The system is beneficial for normalizing the clinical nursing assessment and providing evidence for integrated nursing care.

Objective To evaluate the status of anxiety, depression and cognitive function of elderly patients with diabetic nephropathy(DN), and their relation with nutritional status, in order to provide evidence to improve nutritional status of elderly DN patients.Methods A total of 80 elderly DN patients were investigated with self-rating anxiety scale(SAS),self-rating depression scale(SDS),mini mental state examination(MMSE)for the patients' level of anxiety, depression and recognition and mini nutrition assessment scale(MNA) for patients' nutrition.Results SAS score was (53.52±16.49) with an incidence rate of 62.5%; SDS score was (55.41±13.27) with an incidence rate of 65.0%; MMSE score was (27.36±5.41) and the incidence rate of cognitive function declining was 47.5%. The prevalence of malnutrition, potential malnutrition and the well- nourished were 12.5%, 67.5%, 20.0%,respectively. The MNA grade had a positive correlation with cognitive function, but had a negative correlation with anxiety and depression (P<0.01).Conclusions The correlation among nutritional status with anxiety, depression and cognitive function can not be ignored. Medical personnel should be prepared to take mental intervention to correct the nutritional status of patients, improve their health status and quality of life, as well as reduce negative emotion and pay attention to their psychological status and cognition function.

Objective To analyse the prognosis of 117 newly diagnosed nasopharyngeal carcinoma (NPC) patients underwent intensity modulated radiotherapy (IMRT). Methods From Jan to Nov 2005, 117 NPC patients who were treated by IMRT were enrolled. There were 81 males and 36 females with a median age of 42 years (range 18-76 years). According to Chinese Fuzhou Staging system(1992), 11 cases were Stage I , 15 Stage Ⅱ, 54 Stage Ⅲ and 37 Stage ⅣA. IMRT was carried out with Peacock plan. The prescription dose to the gross target volume(GTVnx) of nasopharyngeal tumor was 68 Gy, that of positive neck lymph nodes (GTVnd) was 60-66 Gy, clinical target volume 1 (CTV1) was 60 Gy, and CTV2 was 54 Gy. Results After a median follow-up time of 48 months (range 10.5-59.5 months), the 3-and 5-year overall survival (OS) rates were 95.7 % and 89.7 %, the disease-free survival (DFS) rates were 91.5 % and 87.2%, and the local-regional control rates were 94.0 % and 91.5 %. Univariate analysis showed the KPS, stage, Fuzhou clinical stage, status of blood platelet before treatment and uric acid after treatment were correlated with OS rate. T stage was the only independent factor of prognosis in the COX stepwise regression model. Conclusion Radical IMRT significantly prolongs the survival of NPC patients. T stage is the only independent prognostic factor for NPC patients.

We developed a high-sensitivity C-reactive protein quantifiable chemiluminescent immunoassay (hs-CRP CLIA). The high-purity native CRP was purified from hepatic cirrhosis patient ascetic fluid by affinity and ion exchange chromatography and used as an immunogen to develop the monoclonal antibodies (mAbs) against CRP. Twenty-two mAbs were identified reactive with CRP in ELISA and 13 of them were reactive in the phosphorycholine ligand capture ELISA. The mAbs 10C5 and 10C11 were selected to develop the hs-CRP CLIA. The linearity and performance of the hs-CRP CLIA was characterized. It was showed not reactive when testing against other serum materials (IgG, hemoglobin and triglyceride). The reliable correlation (R2 > 0.993) was obtained between testing value (RLU/S) and the concentration of human serum CRP calibrator. The linearity fell in the range of 0.04-20.38 mg/L. The assay has good accuracy and reproducibility, the mean recovery was 99% and the precision of the intra- and inter assay was CVs (4.2%-5.8%) and (9.0%-11.5%), respectively. In testing of 90 human sera, this assay performed well and correlated comparably with a commercial hs-CRP ELISA kit. Thus, hs-CRP CLIA is an accurate, reliable, quantifiable assay for detection of high-sensitive C-reactive protein in serum, it may be useful to improve the risk assessment of cardiovascular disease and the prognosis of inflammatory bowel disease.
C-Reactive Protein ; analysis ; chemistry ; Humans ; Immunoassay ; methods ; Luminescent Measurements ; methods ; Sensitivity and Specificity