A total of 100 in-hospital patients with acute stroke were randomly assigned to receive traditional Chinese medicine Radix et Rhizoma Rhei combined with abdominal massage(once daily; study group, n=50) or observation (control group, n=50 ) to prevent from constipation. After 3 days' treatment, 3 patients (6%) in the study group and 44 patients (88%) in the control group developed constipation (χ2=67.482, P<0.01). In addition, no adverse effects occurred in the study group. Our results indicated that traditional Chinese medicine Radix et Rhizoma Rhei combined with abdominal massage might help acute stroke patients to prevent from constipation, achieve earlier health recovery, and improve quality of life.

Dendritic cells(DC)present telomerase reverse transcriptase(hTERT)to T lymphocytes, stimulating specific reactions of cytotoxic T cells and CD4 + T cells. Researches on the relationship between natural killer(NK)cells and telomerase of tumor cells are scarce. DC and NK cells act together promoting antitumor responses and possibly participate in anti-tumor immune responses with telomerase.

Objective To investigate the relationship between serum levels of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMP) and the mortality in patients with malignant middle cerebral artery infarction.Methods A total of 74 patients with malignant middle cerebral artery infarction (MMCAI) was assigned to research group,74 healthy volunteers were recruited to serve as healthy controls,and 242 patients with other cerebral infarction were recruited to serve as case controls.Quality-of-life questionnaire and clinical examination were used to collect the information of participants.Endpoint was 30-day mortality.Results The research group showed higher serum levels of MMP-9,MMP-10,and TIMP-1 relative to the case control group,and the case control group showed higher serum levels of MMP-9,MMP-10,and TIMP-1 relative to the healthy control group (P ＜ 0.05).Compared to survivor ones,non-surviving MMCAI patients showed lower Glasgow Coma Scale score (P ＜ 0.05),higher serum levels of tumor necrosis factorα (TNF-α),MMP-9,MMP-10,and TIMP-1 (P ＜ 0.05).Multiple logistic regression analysis showed that serum levels of TNF-α,MMP-9,MMP-10,and TIMP-1 were associated with 30-day mortality,and the OR were 1.09,1.18,1.13,and 1.32,respectively.Conclusions Serum levels of MMP-9,MMP-10,and TIMP-1 levels in MMCAI patients were associated with mortality,and could be used as an auxiliary index of mortality in MMCAI patients.

OBJECTIVE: To establish a new pattern of ward round record for clinical pharmacist and rational use of drug. METHODS: The new pattern of ward round record was established according to domestic and foreign history pattern, The Principle and Proposed Pattern of Clinical Medication History in China, disease course record. This new pattern focused on patients’ disease and medication changes. A case of cardiovascular department was taken as example. RESULTS: Established ward around record contained two parts, i.e. first page and record chart. CONCLUSION: Established pattern is concise and practical and covers essential medical information. It makes communication more easily between pharmacist and doctors and meets the needs of clinical pharmacy practice.

Objective: To study mental health status in patients with hepatitis and cirrhosis. Methods: Symptom checklist 90 (SCL-90) were assessed to 123 patients with acute and chronic hepatitis and cirrhosis. Results: The factor scores of SCL-90 in those patients were significantly higher than those in Chinese norms (P﹤0.01), especially for somatization, depression, anxiety and hostility factors in patients with chronic hepatitis and cirrhosis. The factor scores existed elevatory tendency according to the severity of disease among the three groups, but the significant difference was only founded in psychotic factor. Multiple factor analysis showed that the type of liver diseases, age and marriage influenced the factor scores. Conclusion: Mental health problems exist in varying degrees in most patients with hepatitis and cirrhosis, and the type of liver diseases, age and marriage play an importance role in the factor scores of SCL-90.

Objective To set up the determining method of quercetinum in Kuding Cha of Ligustrum by HPLC. Method Hypersil ODS2 chromatographic column (4.6 mm?250 mm, 5 ?m) was adopted. Methanol 0.4% phosphoric acid (47∶53) was used as mobile phase, the flow rate was 1 mL/min, and the detecting wavelength was 360 nm. Result The linear range of quercetinum was 0.050 4～1.008 0 ?g, r =0.999 99, and the average recovery was 101.61% (RSD=1.75%, n =5). Conclusion The method is convenient and accurate, and can used be as basis of quality control for Kuding Cha of Ligustrum.

[Objective]To evaluate the efficacy of topical use of 1 g/L tacrolimus eye drops in the treatment of ocular alkali burns.[Methods]A prospective study was performed to evaluate the efficacy of 1 g/L tacrolimus eye drops in the treatment of 12 patients with ocular alkali burns admittedin Zhongshan Ophthalmic Center of Sun Yat-sen University between May 23,2016 and Jan-uary 18,2017. The patients were treated for 1 ~ 18 weeks,with an average of 8.91 ± 5.56 weeks.The degree of ocular alkali burns was evaluated according to Roper-Hall classification system. In this group of patients,there were 2 cases of gradeⅡburns,5 cases of gradeⅢburns and 5 cases of gradeⅣburns. Each patient was given 1 g/L tacrolimus eye drops 2 times a day after admission. The slit lamp microscope and corneal fluorescein sodium staining examination were performed before and 1th week,2nd week,3rd week, 4th week,6th week,8th week respectively after performing topical tacrolimus treatment. Photos were taken to keep records to dynami-cally observe the epithelial repair and anterior segment lesions. Adobe Photoshop CS6 image processing software was used to calculate the area of corneal epithelium repair. The therapeutic effect was evaluated according to the corneal epithelial repair ,visual acuity and complications. The statistical analysis was carried out by SPSS 19.0 software.[Results]4 cases reached good prognosis ,including 2 cases of gradeⅡ and 2 cases of gradeⅢ burns,the corneal epithelium healed and the visual acuity was greater than 0.3. 5 cases had guarded prognosis,including 3 cases of gradeⅢand 2 cases of gradeⅣburns,corneal epithelium healed in 2 cases,corneal epi-thelial repair rate was greater than 50%in 3 cases,in which corneal neovascularization or corneal conjunctiva occurred,and the visu-al acuity was less than 0.3. 3 cases of gradeⅣ burns had poor prognosis:1 had corneal perforation,1 had long term corneal ulcer, and 1 had secondary glaucoma. In 12 cases,the corneal epithelial healing rate reached 99% in 50% patients,and the healing rate was greater than 50%in 75%patients. The visual acuity of 33.3%patients increased by more than three lines,and 66.6%cases had complications. The main factors affecting the prognosis are:The degree of corneal and corneal limbal burns,complication and corneal epithelial repair rate.[Conclusion]1 g/L tacrolimus eye drops can reduce the inflammatory reaction of ocular alkali burns ,reduce the incidence of glaucoma,promote corneal ulcer healing and corneal epithelial repair,and reduce complications caused by corneal lim-bal burns.

Objective: To explore the dignositic characteristics of coronary heart disease (CHD) in perimenopausal women. Methods: The clinical data of 180 patients suspected of CHD were retrospectively analyzed. The risk factors of CHD,clinical characteristics of chest pain, routine electrocardiography,ambulatory electrocardiography and coronary artery angiography (CAA) were compared between perimenopause group and control group. Results: The incidence of CHD increased significantly in control group(13.5%, 10/76) compared to perimenopause group (43.3%, 45/104, P

Objective To establish a TaqMan-MGB fluorescent probe characterized real-time polymerase chain reaction ( qPCR) method for detecting retinoic acid induced genes G ( RIG-G) in human acute promyelocytic leukemia ( M3 ) .Analyze RIG-G expression levels in peripheral blood of both normal persons and M3 patients and explore its diagnosis value for M 3.Methods Methodology establishment study.A detection method and standard curve of TaqMan-MGB real-time PCR were established after designing specific primers and TaqMan-MGB fluorescence probe of human RIG-G gene and using reverse transcription complementary DNA ( cDNA) as a template.The performance of this method was evaluated in specificity, accuracy, precision, analytical sensitivity and interference substances . Twenty clinical specimens with M3 were quantified RIG-G expression so as to evaluate the correlation between peripheral blood and bone marrow samples .Meanwhile , the results of RIG-G expression in peripheral blood of 40 normal specimens and 20 patients with M3 were analyzed by t-test.And receiver-operating characteristic curve ( ROC ) was used to analyze the detection efficiency of M 3.Results There was a good linear relationship between log value of RIG-G standard substance and threshold cycle number ( Ct ) ( standard curve equation:Y=-3.539X+42.952,R2 =0.999).New method was used to detect standard substance . The deviation between observed and expected values was <5% (r=0.999).Three concentration samples (107 ,104 ,101 copies/μl) were selected for precision test.Intra-assay coefficients of variation were 1.38%, 2.31% and 1.38%, respectively , and intre-assay coefficients of variation were 0.71%, 1.17% and 5.07%, separately.All were less than 10%.The sensitivity of this method was 101 copies/μl.There was a good correlation of RIG-G results between peripheral blood and bone marrow in M 3 patients(r=0.996, b=0.973).But there was no significant difference between this two group results (t=0.099, P>0.05). However , there was obvious difference of RIG-G value in peripheral blood between control group and M 3 patient group (U=18,P<0.001), 3.62 ×104(1.61 ×104 -4.90 ×104)copies/μl for controls and 7.10 ×102 (5.43 ×102 -2.21 ×103 ) copies/μl for M3 patients, respectively.Conclusions Successfully establishe a TaqMan-MGB real-time PCR method for detecting RIG-G gene in peripheral blood.The accuracy, precision, sensitivity and specificity are good .It could provide necessary help in early diagnosis and monitor treatment of clinical M3 patients.

Objective To evaluate the serum level of antimicrobial peptide human cationic antimicrobial protein 18 ( hCAP18 ) in non-small cell lung cancer ( NSCLC ) patients and its auxiliary diagnosis and prognosis value.Methods Case-control study was used.The serum level of hCAP18 was measured by enzyme linked immunosorbent assay ( ELISA) in 50 cases with NSCLC patients of department of thoracic surgery and 50 cases healthy people of department of physical examination from January 2011 to January 2012 in Tongji Hospital of Tongji University.The concentrations of hCAP18 in serum of NSCLC patients before and after surgery were analyzed.The sensitivity and specificity of serum hCAP18 for the diagnosis of NSCLC were evaluated using the receiver operating characteristic ( ROC ) curves.Data was analyzed by using the t-test and Log-rank test.Results Serum hCAP18 concentration in NSCLC patients (6 733 ±771.8) μg/L was significantly higher than in healthy controls (253 ±6.9) μg/L (t=8.396, P<0.05) .However, the concentration of hCAP18 showed no significant difference between squamous cell carcinoma and adenocarcinoma[(6 300.0 ±1 221.0) μg/L and (7 074.0 ±1 005.0) μg/L, respectively;t=0.494 2, P <0.05 ] .hCAP18 levels had significantly decreased in serum of NSCLC patients after 30 d surgery compared to preoperative results[from (6 733.0 ±771.8) μg/L to (433.6 ±38.2)μg/L;t=8.512, P<0.05].ROC analysis of serum hCAP18 yielded an AUC (Area under the ROC curve) of 0.931 ( 95% CI =0.884 -0.978 ) with 95% sensitivity and 96.3% specificity, which was higher than the CYFRA21-1[0.873 (95%CI=0.758-0.917)].The relapse rate of NSCLC patients with serum hCAP18≤390.0 μg/L was 12.5%(4/32), while 44.4%(8/18) in NSCLC patients with serum hCAP18>390.0μg/L (χ2 =22.64,P<0.05).Conclusions Detection of serum hCAP18 shows a good sensitivity and specificity for the auxiliary diagnosis of NSCLC. It is possible to be a potential detection index for noninvasive diagnosis and monitoring progression of lung cancer.