Cardio-pulmonary exercise was performed in 43 normal subjects and 175 patients with cardiac disease in order to evaluate the severity of heart failure. AT could be determined easily during bicycle ergometer exercise by measuring minute ventination (V_E), oxygen uptake (V_(O2)) and carbon dioxide output (V_(CO2)). Peak V_(O2) and % AT were also determined, Both AT(ml/min/kg) and peak V_(O2) (ml/min/kg) declined with age, how ever male showed higher values than femal in both indiceS, % AT (determined by the predicted AT values of each age and sex), decreased as class of NYHA progressed (i. e. 85.3?10.3% in class Ⅰ, 74.7?14.1% in class Ⅱ, and 58.8?12.8% in class Ⅲ). The reproducibility of AT was excellent in two testing With in a 3-hour interval. These results suggested that indices from cardio-pulmonary exercise testing, especially AT, were closely related to the pathophysiology of heart failure so that they might be used as an objective parameters and were reliable for evaluation of the severity of heart failure and are sensitive enough to detect the efficacy of therapuetic intervention for heart failure.

Objective To investigate the clinical application value of ultrasound-guided catheter drainage and vacuum-assisted Mammotome system in the treatment of lactational breast abscess. Methods The clinical data of 46 patients with lactational breast abscesses were analyzed retrospectively. All patients were divided into control group (28 patients) and treatment group (18 patients). Homemade irrigation and drainage tube was used in control group,and vacuum-assisted Mammotome system was used in treatment group. The curative effect of 2 groups was compared. Results All patients were cured. The mean healing time in treatment group was (9.5 ± 1.3) d,in control group was (12.5 ± 2.2) d, and there was significant difference (P < 0.01). The rate of breast-feeding in control group was 57.1% (16/28), in treatment group was 13/18,and there was no significant difference (χ2=1.069, P>0.05). All patients recovered well without recurrence. Conclusions Ultrasound-guided catheter drainage should be employed as first line treatment of breast abscess with diameter greater than the 3 cm. If patient′s economic conditions are good and have high requirement on the beauty outcome, vacuum-assisted Mammotome system can be applied as treatment option.

To capture a state of the enzyme in complex with an intact substrate, we developed and adopted a novel freezing method in crystal preparation procedure. Neither the elimination of the catalytically indispensable ligands, nor mutation or modification of the active site is required. At -20 degrees C, we soaked the crystal of 6-phosphate-beta-glucosidase (Bg1A) in the liquor containing p-nitrophenyl-beta-D-glucopyranoside-6-phosphate (pNPbetaG6P). The diffraction data at 2 A resolution was collected and an intact and unambiguous electron density map of pNPbetaG6P was obtained. These results provide an effective method for the research of cryoenzymology and the intermediate state of enzyme-substrate complex in the future.
Cold Temperature ; Crystallization ; Glucosidases ; chemistry ; metabolism ; Substrate Specificity ; X-Ray Diffraction

Objective To discuss the technique and therapeutic effect of pneumatic ballistic lithotripsy for the treatment of bladder calculi under ureteroscope via the outer sheath of resectoscope.MethodsTotally 42 patients with bladder calculi(39 men and 3 women)were treated in our hospital form March 2002 to February 2005.Among the patients,20 cases were complicated with BPH,and 3 had bladder fabrosis.The cystolith was crushed with pneumatic ballistic lithotripsy under an ureteroscope via the outer sheath of resectoscope.For the patients complicated with BPH(20 cases),prostatectomy was performed at the same time.ResultsAll the cases were cured in one session.No massive hemorrhage,bladder perforation,or overhydration occurred after the operation in this series.The patients were followed up for 6 to 18 months(mean 8 months),during which no recurrent cases were found,all the patients had normal urination.ConclusionsPneumatic ballistic lithotripsy via the outer sheath of resectoscope is an effective,simple,accurate method for the treatment of bladder stones.The procedure is superior in less complications and good outcomes.

OBJECTIVE:To investigate the compatible stability of isosorbide mononitrate(ISO) and dopamine(DA) hydrochloride in glucose injection.METHODS:The contents of ISO and DA in the mixture of ISO and DA at 20 ℃ and 30 ℃ under natural illumination within 24 h,and the pH and appearance of the mixture were monitored.RESULTS:No significant change was noted in the concentrations of ISO and DA,the pH and the appearance of the mixture at 20 ℃ or 30 ℃.CONCLUSION:The mixture of ISO and DA in glucose injection was stable at 20 ℃ or 30 ℃,but which should be used up within 18 h after mixing.

Objective·To isolate lipopolysaccharide (LPS) and capsular polysaccharide (CPS) from Porphyromonas gingivalis (Pg) and analyze their ability to induce THP-1 cells to secrete interleukin 8 (IL-8) in vitro. Methods·LPS and CPS were isolated from the polysaccharides by gel filtration chromatography, which had already been extracted from Pg ATCC33277 and SJD4. The isolated LPS and CPS from different strains were used to stimulate THP-1 cells in different doses and for different periods. The level of IL-8 in the cell culture suspension was measured by ELISA. Results·LPS and CPS were successfully separated from the polysaccharides in Pg ATCC33277 and SJD4. The relative molecular masses of LPS and CPS were 20000-50000 and 150000-500000, respectively. LPS and CPS could induce THP-1 cells to produce IL-8 in vitro in a time- and dose-dependent manner. The effect of LPS from ATCC33277 on secretion of IL-8 was greater than that of CPS with the dose of 0.1 μg/mL (P<0.05). However, the difference could only be found at 24 h in SJD4, and the effect of CPS was greater (P<0.05). Conclusion·As a component of polysaccharides extracted from Pg, CPS can induce THP-1 cells to secrete IL-8 in vitro in a time- and dose-dependent manner.

OBJECTIVE:To study the compatible stability of gatifloxacin for injection with tinidazole injection.METHO_DS:Contents changes of gatifloxacin and tinidazole within 48h after mixing were determined by HPLC;and the appearance,pH value were observed and determined.RESULTS:The contents changes for both gatifloxacin and tinidazole were less than 5%. The mixed solution was clear in appearance yet without generation of gas and sediments.No significant changes were noted in pH value,color and smell.CONCLUSION:The mixed solution of gatifloxacin for injection with tinidazole injection is stable at room temperature within 48h and which can be applied in combination.

Nisin is a cationic antimicrobial peptide produced by some lactic acid bacteria. However, expression of nisin resistance protein (NSR) could confer nisin resistance on some non-nisin-producing Lactococcus lactis. To deeply elucidate molecular mechanism underlying NSR-mediated nisin resistance, an NSR mutant with N-terminal 38 amino acid residues deleted (NSR?38) was overexpressed in Escherichia coli by fusion with GST. Purified NSR?38 was obtained through glutathione (GSH) affinity chromatography followed by cleavage of GST tag. Putative proteolytic activity of NSR?38 was determined in vitro against nisin. Antimicrobial activity analysis revealed that nisin lost its bactericidal activity after incubation with NSR?38. Further reversed-phase high performance liquid chromatography (RP-HPLC) analysis indicated that NSR?38 displayed proteolytic activity against nisin, thus inactivating the antimicrobial peptide. The current study paves the way for in-depth functional studies on NSR.

Objective To observe the effect of hyperbaric oxygen (HBO) combined with borneol on the permeability of the blood-brain barrier to sodium valproate in rats.Methods Fifty-six male,healthy Sprague Dawley rats were randomly divided into a control group which received an intraperitoneal injection of sodium valproate (0.1 g/kg),an HBO-1 group to which HBO was administered 0.5 hours before an equivalent injection of sodium valproate,an HBO-2 group where the HBO and sodium valproate were administered together,an HBO-3 group which received HBO 0.5 hours after the injection,a low dosage borneol group (L-borneol) which received 0.125 g/kg of borneol by oral perfusion 0.5 hours before an injection of sodium valproate (0.1 g/kg),a high dosage borneol group which received 0.25 g/kg of borneol 0.5 hours before an injection of sodium valproate (0.1 g/kg) and a combined group which received the HBO-3 group's treatment supplemented with the borneol dose of the L-borneol group.There were 8 rats in each group.Blood and cerebrospinal fluid (CSF) were collected 1.5 h after the sodium valproate injection and the concentrations of sodium valproate were measured by high performance liquid chromatography.Results Compared with the control group,the CSF concentrations of sodium valproate in the HBO-2 and HBO-3 groups had increased significantly more (to 97.43 ± 12.09 mg/L and 100.10 ± 13.54 mg/L respectively).The ratios of the CSF to plasma (C/P) concentrations had also increased significantly more.The CSF concentration of sodium valproate in the H-borneol group (91.09 ± 9.45 mg/L) and the C/P ratio (0.577 ± 0.051) had increased significantly more than in the control group.Compared with the L-borneol group,there were significantly greater increases in the HBO-3 group and the combined group in the concentration of sodium valproate in the rats' CSF.Compared with the HBO-3 group,the average concentration of sodium valproate in the CSF of the combined group (112.43 ± 11.52 mg/L) and the C/P ratio (0.698 ±0.058) had increased significantly more.There was no significant difference among the groups in the plasma concentrations of sodium valproate.Conclusions HBO can increase the permeability of the blood-brain barrier to sodium valproate,at least in rats.Combining HBO with a low dose of borneol has an additional effect.

Objective: To investigate whether the plasmid ?1neo-hgp100 could be expressed and presented in vitro and could protect the immunized mice from B16F10 challenge in vivo. Methods: ?1neo-hgp100 plasmid was constructed in which the DNA sequence encoding hgp100 CTL epitope inserted into CDR3 of ?1-neo vector. The expression of anti-bodized antigen and IFN-? in supernatant was measured by ELISA respectively after transfection J558L with ?1neo-hgp100 and further co-culture of J588L transfacted with ?1 neo-hgp100 and pmel TCR transgenic T cell. After introspleenic inoculation of ?1neo-hgp100, the protective efficacy of the gene vaccine was observed by means of measuring the tumor area every two days. Results: ?1neo-hgp100 could be expressed and presented in vitro, the immunogenecity of CTL epitope of hgp100 was strong enough and could activate gp100 specific T cell, the mice immunized with the gene vaccine could resist the tumor challenging in vivo. The mean survival time was prolonged to 36 days, compared to control group (P