Inflammatory diseases are a group of disorders caused by abnormal activation of the immune system,including autoimmune diseases,chronic inflammatory bowel disease,metabolic inflammatory diseases,and periodontitis.The transcription factor purine rich box-1(PU.1)plays an important role in the development and progression of inflammatory diseases by regulating the development and differentiation of immune cells and related inflammatory signaling pathways.In recent years,studies have gradually revealed the cen-tral role of PU.1 in inflammatory response and highlighted its clinical application value as a potential therapeutic target.This article systematically summarizes the biological features and regulatory mechanisms of PU.1,explores its role in inflammatory response and common inflammatory diseases,and analyzes its clinical application strategies,in order to provide a reference for future research and clinical application.

Objective To investigate the correlation between interleukin 1β gene-511C/T polymorphism of and essential hypertension in the Yi ethnic group of Yunnan province.Methods-511C/T polymorphism of interleukin 1β gene was detected by PCR-RFLP in 85 Yi patients with essential hypertension(EH group)and 106 Yi healthy people(control group)in Shuanghe Township,Jinning County,Yunnan Province.Genotype and allele frequencies were analyzed by SPSS 27.0 software,and association analysis was performed.Results The frequency distribution of CC,CT and TT genotypes at the mutation site 511 of the IL-1βgene in EH group was 18.82%,44.71%and 36.47%,respectively,and it was 5.66%,26.42%and 67.92%in the control group.The difference in genotype frequency between the two groups was statistically significant(P＜0.05).The allele frequency of C and T in EH group was 41.18%and 58.82%,respectively,and the allele frequency of C and T in control group was 18.87%and 81.13%.The frequency difference of alleles between the two groups was statistically significant(P＜0.05).Both genotype frequency and allele frequency found in males and females had statistical differences(P＜0.05).Conclusions The distribution of IL-1β gene-511C/T polymorphism is related to the incident of essential hyper-tension among the Yi ethnic group Yunnan Province,and is the susceptibility gene of the Yi ethnic group to essen-tial hypertension.

Mice ; Animals ; Rett Syndrome ; Longevity ; Methyl-CpG-Binding Protein 2 ; Disease Models, Animal ; Phenotype

Objective: To investigate the transcriptomic changes in primary human oral keratinocytes (pHOKs) after coculture with Prevotella melaninogenica (P.m) and to verify the changes in human oral keratinocyte (HOK) cell lines. Methods: pHOK was isolated and cocultured with P.m for 0, 4 and 24 h. Total RNA was extracted, a gene library was constructed, transcriptional sequencing was performed, differentially expressed genes (DEGs) were analyzed, gene ontology (GO) pathway analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed, and the validation of DEGs was performed by qRT-PCR and Western Blot in the HOK and P.m coculture cell model. Results : 1 788 DEGs were detected between the 4 h group and control group, including upregulated DEGs such as lymphocyte cytosolic protein 1(LCP1), keratin 7 (KRT7) and Cilia and flagella associated protein 251(CFAP251) and downregulated DEGs such as FERM, ARH/RhoGEF and Pleckstrin domain protein 1 (FARP1), WW domain containing transcription regulator 1(WWTR1) and Discoidin, CUB and LCCL domain-containing protein 2 (DCBLD2). 1 832 DEGs were detected between the 24 h group and control group, including upregulated DEGs such as LCP1, complement C1s(C1S), kynureninase (KYNU) and downregulated DEGs such as phosphoserine aminotransferase 1 (PSAT1), FARP1 and FKBP prolyl isomerase 10 (FKBP10). There were 1 090 common differentially expressed genes (cDEGs) in the 4 h and 24 h groups, including LCP1, KYNU and long intergenic nonprotein coding RNA 958 (LINC00958). The GO pathways were mainly enriched in response to lipopolysaccharide and the molecules of bacterial origin and apical part of the cell. KEGG pathway analysis revealed enrichment in the interleukin-17 (IL-17) signaling pathway, tumor necrosis factor (TNF) signaling pathway, Toll-like receptor (TLR) pathway, etc. We verified the expression of a cDEG, Myosin1B (MYO1B), and qRT-PCR and Western Blot analysis showed that MYO1B expression was significantly upregulated between the control group and the P.m cocultured group (P<0.001), and its expression followed a time-dependent and concentration-dependent manner. Conclusion P.m played an important role in the transcriptome of oral keratinocytes.

Age-related laryngeal dysfunction seriously affects swallowing, speech and respiratory function of the elderly and decreases their quality of life.This review summarizes the methods for assessing swallowing, voice and respiratory function associated with laryngeal dysfunction in the elderly, aiming to improve the standards and systems for laryngeal dysfunction assessment in the elderly and to achieve timely detection and treatment of laryngeal dysfunction in the elderly and reduce its negative effects.

Objective To explore the value of primary and Advanced levels of laparoscopic simulating training in different seniority of gynecologic residents.Methods 77 residents in their first to forth training-year were divided into two groups:1-2 year resident and 3-4 year resident,trained with different levels of simulating training plans respectively and then assessed in the Department of Obstetrics and Gynecology in Peking University Third Hospital.Results The qualified rate of primary and advanced simulation training was 85.7％ and 57.1％ respectively.The qualified rate of primary training (80.4％ vs.100.0％,P=0.028) and advanced training (12.5％ vs.57.14％,P=0.000) were significantly different between 1-2 year residents and 3-4 year residents.The operative skills improved significantly in all the residents.In the 1-2 year residents,the scores of the primary training increased more obviously,while in the 3-4 year residents,the scores of the advanced training increased significantly.Conclusion It might be more effective for residents with different seniority to receive different levels of simulating training accordingly,so as to improve their laparoscopic operative skills more effectively.

Objective To observe the effect of comprehensive rehabilitation nursing intervention in patients with hypertensive intracerebral hemorrhage complicated with hemiplegia and its effect on neurological function.Methods A total of 120 hypertensive intracerebral hemorrhage complicated with hemiplegia were selected as research objects,and were divided into control group and observation group according to random number table method,with 60 cases in each group.The patients in the control group were treated with routine rehabilitation,and the patients in the observation group were given comprehensive rehabilitation nursing on the basis of routine rehabilitation.The levels of muscle strength,neurological function and recovery efficacy were evaluated before and 6 months after nursing.Results There was no significant difference in muscle strength between the two groups before treatment (P ＞ 0.05).After the implementation,muscle strength of two groups were increased,and the observation group was higher than the control group (P ＜ 0.05).The levels of S100B and MBP were lower,and the level of BDNF was higher than nursing before and that in the control group (P ＜0.05).The score of NIHSS was lower and the score of Barthel index was higher than nursing before and that in the control group (P ＜0.05).Conclusion Comprehensive rehabilitation care intervention can effectively enhance the patient's muscle strength level,improve limb function,reduce neurological damage,improve self-care ability,and reduce the impact of sequelae for patients with hypertensive intracerebral hemorrhage complicated with hemiplegia.

Objective To observe the effect of comprehensive rehabilitation nursing intervention in patients with hypertensive intracerebral hemorrhage complicated with hemiplegia and its effect on neurological function.Methods A total of 120 hypertensive intracerebral hemorrhage complicated with hemiplegia were selected as research objects,and were divided into control group and observation group according to random number table method,with 60 cases in each group.The patients in the control group were treated with routine rehabilitation,and the patients in the observation group were given comprehensive rehabilitation nursing on the basis of routine rehabilitation.The levels of muscle strength,neurological function and recovery efficacy were evaluated before and 6 months after nursing.Results There was no significant difference in muscle strength between the two groups before treatment (P ＞ 0.05).After the implementation,muscle strength of two groups were increased,and the observation group was higher than the control group (P ＜ 0.05).The levels of S100B and MBP were lower,and the level of BDNF was higher than nursing before and that in the control group (P ＜0.05).The score of NIHSS was lower and the score of Barthel index was higher than nursing before and that in the control group (P ＜0.05).Conclusion Comprehensive rehabilitation care intervention can effectively enhance the patient's muscle strength level,improve limb function,reduce neurological damage,improve self-care ability,and reduce the impact of sequelae for patients with hypertensive intracerebral hemorrhage complicated with hemiplegia.

Gallbladder carcinoma is the most common malignancy in biliary tract.Early dissemination,rapid and silent progression and delayed diagnose could portend a dismal prognosis.The 5-year survival rate is less than 10％.Clarifying the etiological feature can improve the early diagnosis.Comprehending surgical indications of benign lesions can provide the methods for rational prophylactic resections.Standardized treatment of accident gallbladder carcinoma could improve the patients' prognosis.Efficient serologic markers could be used for early diagnosis and screening.