Object To study the chemical constituents of Pueraria omeiensis Tang et Wang. Methods Isolation and purification were repeatedly carried out on silica gel column chromatography, Sephadex LH-20, and preparative HPLC. The structures were identified and elucidated by physicochemical properties and spectral analysis. Results One compound was elucidated as sophoracoumestan A (Ⅰ) from the acetic ether section, and 15 compounds were obtained from the n-butanol section. They were ononin, (Ⅱ), daidzein 7,4′-diglucoside (Ⅲ), daidzein 7,4′-glucoside (Ⅳ), 3′-methoxy puerarin, PG-3 (Ⅴ), 3′- hydroxypuerarin, PG-1 (Ⅵ), mirificin (Ⅶ), 8-methyl retusin 7-O-glucoside (Ⅷ), daidzein (Ⅸ), daidzin (Ⅹ), genistin (Ⅺ), puerarin ( ⅩⅡ), octacosanoic ( ⅩⅢ), allantoin ( ⅩⅣ), glycerol 1-monopentacosanoate ( ⅩⅤ), daucosterol, ⅩⅥ), respectively. Conclusion Besides compounds Ⅸ- ⅩⅡ, ⅩⅥfrom the acetic ether section, the others are first obtained in P. omeiensis, and compounds Ⅰ, Ⅳ, Ⅷ are found in the plants of Puerarea DC. for the first time.