The callus of a "Luling"strain of Plantago asiatica was induced by 2, 4-D. When the induced callus was transferred to 2, 4-D free MS medium,budgensis was observed about 2 weeks afterward only by the addition of 6-BA alone. Rootgensis of the bud was stimulated by NAA and 6-BA added to the MS medium thereafter.Finally, the completed plantlet could be obtained.