BACKGROUND: Chitin and its derivatives possess many biological activities such as immune modulation and promoting wound healing and hemostasis. As one of the derivatives of chitin, the effect of N-acetylglu cosamine on fracture healing has not yet been acknowledged.OBJECTIVE: To observe the changes in osteoblastic activity and the expression of bone morphogenetic protein (BMP) in response to the intervention with N-acetylglucosamine during the fracture healing. DESIGN: A randomized controlled experiment SETTING: Department of Anatomy, Medical College of Qingdao University.MATERIALS: This experiment was carried out in the Laboratory of Neuroanatomy, Medical Colloge of Qingdao University from March to August 2002. Seventy healthy New Zealand rabbits were randomized into four groups, namely saline group (n=23), Jiegupian group (n=23) and N-acetyl glucosamine group (n=24).METHODS: The rabbits were subjected to operations to induce 3-mm bone defect in the middle segment of the right radius with subsequent treatments with saline (1.00 mL/kg), Jiegupian (1.00 mL/kg) and N-acetyl glucosamine (0.28 g/kg) via intragastric administration on a daily basis till the time of tissue sampling at postoperative days 9, 17, 30 and 42, respectively. X-ray examination and HE staining were performed to observe the healing process, and the expression of BMP was assessed immunohistochemically.MAIN OUTCOME MEASURES :① General condition of the rabbits after operation; ② Fracture healing process revealed by X-ray at the specified time points; ③ Expression of BMP between the fracture ends at each time point; ④ Results of histological observation.RESULTS: According to intent-to-treat analysis, 70 rabbits entered the data analysis. X-ray revealed better fracture healing in N-acetylglucosamine group than in the ither two groups at postoperative days 9, 17, 30 and 42(P ＜ 0.05). Compared with saline group, the expression of BMP protein in Jiegupian group and N-acetylglucosamine group was increasing at postoperative 9 and 17 days, particularly in the latter group (P ＜ 0.05), but decreased at postoperative 30 and 42 days. Histologically, the gap between the fracture ends reduced in N-acetylglucosamine group at postoperative 9 days with the appearance of ()bmogenous bone trabecula an orderly arrangement of osteoblasts; in saline group, the gap was greater and contained less or even no bone trabecula, with much less, if any, osteoblasts.On postoperative day 30, the bone defect were completely filled by bone trabecula in N-acetylglucosamine group, with the bone trabecula almost communicating with the bone marrow cavity, and the osteoblasts surrounding the bone trabecula were arranged orderly with osteoclasts seldom observed; in saline group, the defect was substituted mostly by bone trabecula with breaches, and the chondrocytes could be observed scarcely. On postoperative day 42, the bone plate woven bone formed primarily at the fracture ends in N-acetylglucosamine group, with completely communicating marrow cavity and numerous active osteoclasts, and osseous lacuna can be observed; in saline group, the defect was filled with bone trabecula but clear fusion lines were not seen, and communication of the bone marrow cavity was nearly achieved with orderly arranged osteoblasts and presence of the osteoclasts.CONCLUSION: N-acetylglucosamine can accelerate fracture healing possibly by promoting the osteoblastic activity and increase the expression of BMP during the early period of fracture healing.

Objective: To explore the possible relationships of adolescent personality traits, personality deviation and parental rearing pattern. Methods: 393 students from a high school in ShanDong were tested by PACL and EMBU. Re- sults: Generally, adolescent personality traits showed different profiles while factors of EMBU varied in different levels; Antisocial personality deviation was greatly influenced by parental warmth and understanding factor, father's punishment and rigor, father's overprotection. Schizoid personality deviation was mainly influenced by parental warmth and under- standing. Passive- aggressive personality dysfunction was influenced by father's warmth and understanding. Conclusion: Adolescent personality deviation is greatly influenced by parental rearing.

Objective To establish a framework of indexes for assessing the composite results in TCM hospitals so as to promote their development. Methods Such methods and techniques as literature review, descriptive study, Delphi expert consultation, step analysis, composite index, weighted rank sum ratio, and correlation analysis as used by Kendall and others were adopted. Results The current situation regarding the allocation and utilization of health resources in a certain city was found out and a framework of indexes for assessing the composite results in TCM hospitals and the demarcation line for dividing the results into grades were defined. Conclusion The development of TCM hospitals in the city has reached a certain scale, the feature of TCM specialties forms a new growth point, and the framework of indexes for the assessment of composite results in TCM hospitals needs constant perfection.

To observe the curative effect and the hemodynamic change of cervical vertigo treated by acupuncture, 66 patients were randomly divided into two groups: group A in which acupoints Yuzhen (BL 9) and Fengchi (GB 20) were used and Group B in which Cervical Jiaji points (C4-6) were used. Before and after treatment, the symptom of the cervical vertigo and the grade variety of function and the change of Color Doppler Flow Imaging (CDFI) of vertebral artery were observed. After treatment, the grade in two groups increased, there was a remarkable difference between two groups(P ＜ 0.05); after treatment, the grade of vertigo degree increased, there was a remarkable difference between two groups(P ＜ 0.05); needling Yuzhen (BL 9) and Fengchi (GB 20) can remarkably improve the symptom of cervical vertigo and vertebral artery blood flow rate, and the curative effect was superior to needling Jiaji points.

Objective To investigate the clinical significance of serum procalcitonin (PCT),C reactive pro-tein(CRP),and lymphocyte subsets CD +19 CD +23 in children with Kawasaki disease (KD).Methods From August 2013 to July 2015 in hospital,30 cases of acute phase KD were selected.According to cardiac color Doppler ultra-sound results,30 patients were divided into coronary artery disease (CAL)group and non coronary artery disease (NCAL)group.The intravenous immunoglobulin (IVIG)before and after treatment,serum PCT,CRP,CD +19 CD +23 were detected.And 30 healthy children were selected as normal control group.Serum PCT was detected by chemilumines-cence immunoassay,CRP was determined by immune latex ratio method,CD +19 CD +23 was measured by flow cytometry. Results The levels of PCT,CRP and CD +19 CD +23 were (1.37 ±0.39)μg/L,(52.24 ±12.99)mg/L,(25.45 ± 11.06)% respectively in KD before IVIG treatment,which were significantly higher than those of KD after treatment [(0.49 ±0.24)μg/L,(37.48 ±6.27)mg/L,(17.23 ±1.97)%]and the control group[(0.05 ±0.00)μg/L, (16.08 ±5.21)mg/L,(15.76 ±2.39)%],the differences were statistically significant (t =6.108,5.983,8.172, all P <0.01).In CAL group,PCT[(1.75 ±0.53)μg/L,(65.95 ±14.55)mg/L]and CRP[(0.93 ±0.42)μg/L, (26.18 ±6.71)mg/L]were higher than NCAL group,the differences were statistically significant (t =5.504,5.621, all P <0.05).While CAL group CD +19 CD +23 [(24.70 ±11.86)%]and NCAL group[(25.79 ±10.59)%]had no statistically significant difference (t =0.559,P >0.05 ).Conclusion PCT,CRP and CD +19 CD +23 can be used as important index for early diagnosis and prediction of KD,the levels of CRP and PCT have clinical significance in the assessment of coronary artery lesions.

Objective To investigate the expression of histone acetylated H3 and H4, methylated H3K4 and H3K9 in diffuse large B-cell lymphoma (DLBCL). Methods The expression of histone acetylated H3 and H4, methylated H3K4 and H3K9 were examined by SP immunohistochemistry technique in lymphoid tissue of 40 cases with DLBCL and 16 cases with proliferative lymphadenitis. Results The expression of histone acetylation of H3 and H4 were lower than that in proliferative lymphadenitis. Histone methylated H3K4 was lower in expression and H3K9 was in higher expression. There was a positive correlative expression between the global histone acetylation of H3 and H4, the global histone acetylation of H3, H4 and histone methylation of H3K4. Conclusion Improper modification of histone acetylations and methylations may play an important role in pathogenesis in DLBCL.

Objective To evaluate the distribution and drug resistance of bacteria causing neonatal infectious pneumonia in Jiaxing,and to provide a therapy for clinical doctor to make a correct diagnosis,choose reasonable anti-biotics and avoid abuse of antibiotics.Methods Took expectoration from trachea in condition of asepsis to conduct culture and perform drug-sensitive test from 3025 cases.Results Totally 1 156 strains of aerobic bacteria were iso-lated.875 strains were gram negative bacilli(75.7%),269 strains were gram positive cocci(23.3%),and 12 strains were fungi(1.0%).Klebsiella pneumoiae,Escherichia coil,Acinetobacter baumanni,Enterobacter cloacae were com-mon in gram negative bacilli( respectively 178 cases,151 cases,87 cases,113 cases) .The proportion of the Staphylo-coccus aureus was the largest in gram positive cocci(245 cases) .The results showed that gram-negative bacilli were resistant to cefazolin, ampicillin, piperacillin and sensitive to meropenem, imipenem, piperacillin-tazuobatanna and cefoperazone-sulbactam.The drug resistance was severe of ESBL-positive.Staphylococcus aureus was resistant to penicillin, ampicillin, erythrocin, clindamycin and sensitive to linezolid, vancomycin, nitrofurantoin.Conclusion Gram-negative bacilli are the main bacteria in neonatals with infectious pneumonia.The drug resistance is severe.It is important to make a standard management and isolation.

Objective To observe the curative effect of head hypothermia combined with mouse nerve growth factor in the treatment of neonatal moderately severe hypoxic ischemic encephalopathy (HIE).Methods 50 cases of severe HIE were randomly divided into observation group and control group,with 25 cases in each group.Both two groups were given the conventional treatment.The observation group was given head hypothermia treatment in 6 hours after born,to maintain the nasopharyngeal temperature (34.0 ±0.5)℃,anal temperature (35.5 ±0.5)℃,72 hours continuously.At 96 hours after the birth,the nerve growth factor was given.The control group did not give the head mild hypothermia treatment.At 96 hours after birth,the nerve growth factor (methods,dosage and treatment were the same as the observation group)was treated with nutrition and brain nerve.After treatment,the improvements of heart rate,muscle tension,convulsions and disturbance of consciousness were observed in two groups.After 3 days,2 weeks and 4 weeks,the neonatal behavioral neurological assay (NBNA),1 and 3 months after birth,the outfit cranial MRI plain scan and brainstem auditory evoked potential (BAEP)were evaluated.Results NBNA scores of the two groups were compared in 3 days,2 weeks and 4 weeks after birth,the differences were statistically significant (t=2.53, 2.89,3.23,all P<0.05).In the observation group,the abnormal brain MRI was significantly less than the control group,the difference was statistically significant (χ2 =24.125,P<0.05).In the observation group,the number of abnormal auditory evoked potential was significantly less than that of the control group,the difference was statistically significant (χ2 =21.312,P<0.01 ).Conclusion Head hypothermia combined with mouse nerve growth factor therapy for the treatment of neonatal moderately severe HIE has protective effect,it can improve the treatment efficacy, reduce the long-term neurological sequelae,and without adverse reaction.

BACKGROUND:Using three dimensional (3D) reconstruction techniques,any part of body is accessible to visual observation.However,reports concerning 3D reconstruction of craniofacial vascularity based on PC are few in China.OBJECTIVE:To explore the method and the application values of reconstructing a digital 3D model of craniofacial vascularity based on the data of CT strengthening scanning.METHODS:CT strengthening scan images from a healthy volunteer in DICOM format were imported into Mimics 10.01 software and craniofacial blood vessels were reconstructed with the technique of thresholding,editing and 3D region growing.RESULTS AND CONCLUSION:The 3D digital model of craniofacial blood vessels was obtained.This model could be zoomed and rotated randomly and displayed the spatial positions and adjacent relationships of different anatomical structures,also the reconstructed structures could be measured in 3D space.The 3D digital model of craniofacial blood vessels can be reconstructed conveniently and quickly with Mimics software on PC,and also it can bring morphological reference to human anatomy teaching,clinical neurosurgery,oral and maxillofacial surgery,as well as image diagnosis,and will be helpful to generate a virtual plateform in craniofacial surgery.

Objective To investigate the expression and its clinical significance of bronchial asthma treat-ment in different periods of peripheral blood lymphocyte CD +19 CD +23 .Methods Peripheral blood CD +19 CD +23 expres-sion and IgE levels in serum of 28 cases of children with bronchial asthma and 28 healthy children was detected by electrochemical detection and flow cytometry assay method,the detection results were analyzed.Results The results of serum CD +19 CD +23 in children with asthma,the level of total IgE were (32.51 ±5.08)%,(995.22 ±576.33)IU /L respectively,which were higher than those of the control group(21.29 ±8.68)%,(100.85 ±36.43)IU /L,there were significant differences between the two groups(t =5.846,8.334,all P <0.01);treatment after 1 months,3 months, 6 months CD +19 CD +23 expression rates were (29.37 ±4.82)%,(26.20 ±4.58)%,(23.69 ±4.54)%,total IgE lev-els were (745.41 ±452.89),IU /L(553.23 ±345.26),IU /L(405.87 ±267.96)IU /L,before and after treatment were decreased,the differences were statistically significant(CD +19 CD +23 :F =3.367,IgE:F =26.740,P <0.01 ). Conclusion Peripheral blood lymphocyte CD +19 CD +23 and IgE can reflect the condition of the asthma children,but compared to IgE,CD +19 CD +23 can better reflect the activation of eosinophils and airway inflammation,serum CD +19 CD +23 levels can be used as a diagnosis of asthma,disease status and guide the indicator to determine the treatment of inflammation.