AIM: To determine epigoitrin and 2,4(1H,3H)-quinazolinedione in Radix Isatidis and its preparation by HPLC-DAD. METHODS: HPLC was carried out with an Agilent 1200,equipped with ZORBAX SB-C_(18) column(4.6 mm?250 mm,5 ?m).The mobile phase of acetonitrile:(0.1% phosphoric acid+0.03% triethylamine)aqueous solution=12∶88.The flow rate was 0.7 mL/min.The detection wavelength was set at 224 nm.The column temperature was at 30 ℃. RESULTS: The linear ranges of epigoitrin was 0.040 9 ?g-1.103 8 ?g;2,4(1H,3H)-quinazolinedione was 0.010 6 ?g-0.169 6 ?g,The recoveries of epigoitrin and 2,4(1H,3H)-(quinazolinedione) were 98.44%(n=9),with RSD of 1.43% and 98.67%,with RSD of 1.65%,respectively. CONCLUSION: The method is simple,sensitive and reliable.It can be used for quantitative determination of Radix Isatidis and its preparation.

Immune homeostasis is among the mechanisms of keeping organisms healthy and capable of responding to unfavorable insults either from exogenous or endogenous environments.In recent years,major progress has been made in basic immunology as spotlighted by innate immunity,characterization of novel subclasses of dendritic cells,T or B lymphocytes,and modulation by microRNA (miRNA),long non-coding RNA (LncRNA) and epigenetics.Meanwhile,the eye poses an organ with complex structure and function with specific requirement for immune processes.Pathogenesis or treatment of major ocular disorders involves transplantation immunology,infection immunology,autoimmunity,hypersensitivity,etc.Hence progression in basic immunology lends valuable insights to scientists or ophthalmologists,helping them to understand ocular immunology concerning eye diseases.This review briefs recent updates and future directions in the basic immunology,aiming to provide more reference to the society of ocular immunology.

Two courses teaching has been in difficult situation in colleges for a long time.Most colleges raise the two courses teaching by way of social practice.However the class is the main channel in the two courses teaching.So only by strengthening the appeal of class teaching and inspiring college students' study enthusiasm can we improve their cognition and thinking ability and unify their thoughts.Thus the class can become the main channel of university students' ideological and political education actually.

OBJECTIVE:To investigate the clinical efficacy of azithromycin in the treatment of mycoplasma pneumonia in children.METHODS:240 cases of mycoplasma pneumonia in children in our hospital recent years were analyzed.In control group,120 children were treated with erythromycin;in treatment group,120 children were treated with azithromycin.RESULTS:In control group,total effective rate was 81.67%;in treatment group,total effective rate was 95.00% (?2=5.18,P

Autosomal dominant polycystic kidney disease (ADPKD) is one of the most common human hereditary disorder characteristic of development of bilateral multiple fluid-filled kidney cysts. Accumulated evidence has suggested that primary cilium of renal epithelial cell plays a key role in cystogenesis. In this article we will give an overview on the basic information about polycystic kidney disease (PKD) and summarize the recent progresses in studies of regulation of polycystin-1 and -2 trafficking to cilia. We will also discuss the possible role of trafficking defects of polycystins on the pathogenesis of ADPKD.

Bases on the changing idea and management policy,the out-patient service process rebuilding focuses on the patient,makes use of the information technology,imports the theory of operation process reforming,reconsiders and redesigns the operation process of the out-patient department,and improves the medical quality and service.The digitizing procedure in out-patient service is the important base of process rebuilding.In the paper,we try to discuss how to improve the digitizing procedure in out-patient service and rebuild the service process.

Objective To explore the correlation factors of cranial magnetic resonance diffusion weighted imaging(DWI) in patients with acute cerebral infarction.Methods A total of 350 patients with acute cerebral infarction who underwent MR imaging were divided into DWI positive group and negative group according to whether the first diffusion weighted imaging was abnormal or not.The clinical and radiological data of the two groups were retrospectively collected and analyzed.patients with negative DWI on initial MRI were re-examined 1 week later.Result Thirty-four(9.7%) patients were DWI-negative in 350 patients with acute cerebral infarction on initial MRI,and 5 patients in DWI-negative group had positive lesions on the re-examine MRI.Multivariate logistic regression analysis indicated the patients with first blood pressure of admission≥ 140/90 mmHg(P=0.033),first blood glucose of admission≥ 7.0 mmol/L(P=0.028) and the time from the onset to initial MRI>24 h were more likely showed DWI-positive on initial MRI(P=0.013).Patients with posterior circulation infarcts were more likely to have negative DWI findings than patients with anterior circulation infarction(P=0.001).Conclusion Some patients with acute cerebral infarction may show DWI negative results on initial MRI,which is related with the time from onset to initial MRI and the site of cerebral infarction.Patients with posterior circulation infarcts are more likely to have first negative MR findings than patients with anterior circulation infarction.Patients with elevation of the admission blood pressure or blood glucose are more likely to have DWI positive lesions.Patients with acute cerebral infarction within the time window should received be thrombolytic therapy after carefully assessment to avoid delay in treatment even if the DWI negative results.

Objective To explore the expression of Ang -2 and CD105 in breast cancer and their po-tential associations with the cancerization and progression of breast cancer .Methods Thirty patients with breast cancer ( cancerous tissue ) ,15 intraductal papilloma cases ( precancer tissue ) and 15 normal breast tissue were col-lected for each group ,respectively .Immunohistochemistry ,western blot and RT-PCR techniques were used to ex-amine the expressions of Ang -2 and CD105 in cancerous breast tissue ,precancer breast tissue and normal breast tissue at both protein and mRNA levels .The possible correlations of Ang -2 and CD105 expression with clinico-pathological characteristics of the breast cancer were analyzed .Results The expressions of CD105 and Ang-2 in cancer and precancer tissues were higher than in normal tissues at both protein and mRNA levels ,and the trend of their expressions was increased from normal to precancer ,and cancer tissue .The expressions of CD 105 and Ang-2 protein and mRNA were positively correlated with tumor size ,invasion,lymph node metastasis,and negatively correlated with the differentiation of the cancer .Conclusion CD105 and Ang-2 are involved in the canceriza-tion and pregression of breast cancer .These two biomarkers can serve as two useful indicators in assisting diagno-sis and prognosis of breast cancer .

The needles were inserted in the center of inferior margin and on the left and right sides of patella. Then moxibustion with warming needle and electroacupuncture were used respectively to treat 25 cases of patellar ligament strain. The effective rates were 96.0% and 69.6% respectively.