Objective To investigate online dating status and human immunodeficiency virus (HIV)infection among men who have sex with men (MSM)in Chongqing,and provide basis for effective intervention in HIV infec-tion among MSM.Methods 206 MSM in Chongqing were performed structured interview and questionnaire survey, MSM who didn’t conduct HIV testing within half a year were performed laboratory screening,sociological popula-tion characteristics and influencing factors of HIV in MSM were analyzed.Results The average age of 206 MSM was (22.08±1 .81)years old,137 (66.50%)received college education and above,HIV infection rate was 21 .36%(44/206).HIV infection rate in MSM who had no fixed partners was higher than those who had fixed partners;the more sexual partners they had,the greater the risk of HIV infection they got;HIV infection rate in MSM who had sex with unfamiliar persons or strangers was higher than those who had sex with familiar partners;the higher rate of condom use in sexual behavior,the lower rate of HIV infection was,there were significant differences among the groups(all P <0.05).Condom use among MSM with different educational levels was significantly different(all P <0.05).Conclusion HIV infection rate in MSM is high in this city,it is necessary to strengthen education about health,network partner,and behavioral intervention,reduce the bridge population connecting MSM network,es-tablish social support system of homosexuality,and reduce the risk of HIV infection in MSM.

In this paper,a time-frequency approach is used for the recognition and analysis of ECG signals for a normal or an abnormal patient.An iterative deconvolution procedure is applied to calculate the time-frequency representations.This method can enhance the resolutions of the signals.

Objective To examine the current status of the sexual behavior among out‐of‐school adolescent men who have sex with men(MSM)and analyze the influential factors of AIDS infection in order to provide countermeasures.Methods The software SPSS 16.0 was used for cross table analysis and chi square test of questionnaire data and HIV detection data.Results The HIV infection rate was 21.36%.The household registration ,the only child ,living state ,coming out of the closet ,fixed sex‐ual partner ,the way they know sexual partners had no significant effects on HIV infection(P>0.05).Having multiple sexual partners(P<0.05) ,unfamiliar with sexual partners(P<0.05) ,having sex with network friends(P<0.05) ,no condom use in sexual life(P<0.01) ,having no regular HIV testing(P<0.01)were significantly related to HIV infection.Conclusion Reduc‐ing the sexual behavior among out‐of‐school adolescent MSM ,carrying out HIV counseling and testing ,promoting the use of condoms contribute to the reduction of HIV infection.

Objective: To study the effect of HSVtk/GCV system on ascites tumors. Methods: Using the methods of modified XTT assay, animal experiment, transmission electron microscopy (TEM ) and flow cytometric ( FCM) assay. Results: The sensitivity of P388tk cell was about 37-fold than that of their patental P388 cell. Furthermore we found P388tk cells exhibited potent bystander killing. In vivo , the growth of tumors, which were produced by injecting P388tk cells or a mixture of 50% P388tk cells and 50% P388 cells into DBA/2 mice, was inhibited , furthermore mice survival periods were prolonged contrasted to control groups. Conclusion: HSVtk/GCV suicide gene system could effectively killed the HSVtk-gene-positive P388tk cells and nearby HSVtk-gene-negative cells by the bystander effect in vitro and in vivo.

ObjectiveExplore the pathogeny of cell immunity of severe aplastic anemia(SAA),also appraise the effect and prognosis value of T cell subset change in the treatment of SAA.MethodTake normal persons as control,combine CD_3,CD_4,CD_8 with human being peripheral blood monocytes,analyse with flow cell method the cells of CD~+_3,CD~+_4,CD~+_8 in monocyte and CD~+_4/CD~+_8.Compare and analyse T cell subset in 3 groups before and after treatment.ResultCompared with control,there’s obvious meaningful difference in T lymphocytesCD~+_3 and CD~+_8,and CD~+_4/CD~+_8(P

Objective To understand the total suspended particulates(TSP)in ambient air and their characteristics on metallic elements in Xi an.Methods 96air samples were collected from the industrial area,industry-traffic mixed area,heavy traffic area and control area in Xi an during the heating season and unheating season,2001.The concentra-tions of TSP in ambient air and the contents of Pb,Mn,Cr,Ni,Cd in inorganic extracts of TSP were determined.Re -sults The concentration of TSP in ambient air(0.5865mg /m 3 )during heating season was significantly higher than that(0.3535mg /m 3 )during unheating season(P

Objective To analyze the relationship between UDP-glucuronosyltransferase 1A1 (UGT1A1)gene polymorphism and delayed diarrhea caused by FOLFIRI treatment.Methods Two hundred and one blood samples were taken from patients with metastatic digestive tract tumor before chemotherapy by FOLFIRI and then the UGT1A1 * 28 genetic polymorphism was performed.All the cases treated with FOLFIRI were chosen to be observed and recorded by situation of the delayed diarrhea during chemotherapy,and to analyze the relationship between UGT1A1 * 28 genetic polymorphism and grade 3 and 4 delayed diarrhea.Results The distributions of the genotypes in 201 metastatic digestive tract tumor patients were as follows:UGT1A1 * 28 wild-type genotype TA6/6 (155,77.11％),heterozygous genotype TA6/7 together with homozygous genotype TA7/7 (46,22.89％).In the 201 cases,the incidences of grade 1 and 2 delayed diarrhea in the patients carrying wild-type genotype and mutant type were respectively 45.16％ (70/155),39.13％ (18/46).The incidences of grade 3 and 4 delayed diarrhea were respectively 9.68％ (15/155),19.57％ (9/46),with no statistical difference (x2 =3.318,P =0.190).Conclusion The UGT1A1 * 28 polymorphism TA6/7 or TA7/7 can not increase the risk of grade 3 or more severe delayed diarrhea for the patients with metastatic digestive tract tumor after receiving FOLFIRI treatment.

After severance the ventral spinal roots in 10 dogs, the distribution of the anterograde degeneration was studied by means of Nauta's technique with the following conclusions:1. After sectioning the ventral roots, degenerating fibers (including preterminal and terminal fibers) appeared regularly in the spinal cord and medulla oblongata. This confirms that there are afferent fibers which project into the central nervous system via the ventral spinal roots.2. These degenerating fibers ascended not only in the posterior funiculi, but also in the gray matter, so that the preterminal and terminal degenerations of the terminal branches or collateral branches were found in the columna grisea posterior of the thoracic and cervical segments above the surgically operated segments of the spinal cord.3. The preterminal and terminal degenerations were found in the reticular areas of the nucleus gracilis of the medulla oblongata and around the non-cluster cells.4. Degenerating fibers were unequally distributed, most of them located ipsilaterally. The degenerating fibers decreased in number in rostral segments.5. Afferent fibers in ventral roots differ from those in dorsal roots in distribution:a) From the operated segment and to far more rostral segments, afferent fibers of the ventral root were distributed in a more medial position in the spinal dorsal gray column, viz, "medial tendency", and then ascended after interposing into the funiculi gracilis. This is different from the afferent fibers of the dorsal root in the control group which were distributed in "equal tendency" in the dorsal column and took a "lateral addition fashion" of entering the funiculi gracilis.b) Apparently, much more afferent fibers in ventral roots projected contralaterally than those in the dorsal roots.c) It was also characteristic that the afferent fibers in ventral roots projected further toward the rostral segments of the spinal cord.

Objective To investigate and analyze the expression of α-SMA and PCNA in abdominal aorta and renal artery in obstructive sleep apnea syndrome(OSAS)combined with hypertension rats. Methods 36 SD rats were randomly divided into normal control group and model group. The model group was subjected to intermittent hypoxia condition for 8h everyday. Rat tail artery pressure was monitored every week and all subjects were sacrificed at 12th week. The level of TNF-α, IL-6, IL-10 and hs-CRP expression in serum were measured by ELISA method. Immunohistochemical analysis was performed on α-smooth muscle actin (α-SMA) and proliferating cell nuclear antigen (PCNA). Results Compared with control group,model rats blood pressure increased significantly (P < 0.01) when exposed for 4 weeks under intermittent hypoxia condition,and reached the peak value of 186mmHg at 12 weeks. The level of TNF-α, IL-6 , IL-10 and hs-CRP in serum were higher than in control group (P < 0.01). Immunohistochemical staining showed that the expression of α-SMA in abdominal aorta decreased more significantly than control group (P < 0.05),while there was a stronger positive expression of PCNA in model group than those in control (P < 0.05). In addition,compared with control rats, model rats showed a higher expression of α-SMA and PCNA significantly in renal artery(P < 0.05). Conclusion The intermittent hypoxia condition could result in higher blood pressure,while the different expression of α-SMA and PCNA illustrate that high pressure show different reconstruction performance in different vascular,underlying different molecular mechanism.

BACKGROUND: Studies have demonstrated that exogenous basic fibroblast growth factor (bFGF) has intensive effects to promote proliferation of gingival fibroblasts (GFs) cultured in vitro and the heeling of gingival wounds. OBJECTIVE: To investigate the effects of bFGF gene transfection on the biological performance of Beagle canine GFs. DESIGN, TIME AND SETTING: An observation and comparison in vitro experiment regarding cells was accomplished in Centre of Cell Biology and Development of Fujian Medical University and Department of Comparative Medicine in Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA from April to September of 2008. MATERIALS: Four beagle dogs, male, 12 months old, weighing 10-13 kg were used in this experiment, plRES2-EGFP-bFGF plasmid containing full-length human bFGF gene cDNA was constructed and conserved by our institution. METHODS: Free gingiva of the 2nd, 3rd and 4th premolars were excised from left upper jaw of Beagle dogs, dnsed with aseptic phosphate buffer four times, then cut into pieces and digested with 2.5 g/L pancreetin for 2 hours at 37 ℃. After the cantrifugation and supernatant removal, DMEM containing 10% fetal bovine serum was added to incubate on 6-well plate with coverlips in 5% CO2 incubator at 37 ℃. Logadthrnically growing cells were digested and passaged. GFs were transfected with pIRES2-EGFP-bFGF plasmid using liposome mediated method, while vacant plasmid transfection and un-transfection group served as controls.MAIN OUTCOME MEASURES: Proliferation and apoptosis feature of the GFs were evaluated by M'rE and AOEB, respectively. The activity of alkaline phosphatase was assayed by chemical coledmetry. RESULTS: All of three groups cells entered log phrase on three days after transfection. MTT results showed that the proliferation of GFs transfected with bFGF was greater than cells transfected with vacant vector and untransfected cells (P < 0.05). AO/EB dyeing showed the apoptosis rate of GFs transfected with bFGF was reduced compared with other two groups (P < 0.05). After bGFG gene transfection, the ALP activity remained unchanged and there was no significant difference compared with untransfected cells.CONCLUSION: The transfection of bFGF gene to GFs can promote the proliferation of GFs and depress the apoptosis. No promotion is present with regard to the GFs differentiation.