Some biological and biochemical characteristics of metacercaria of Euparagonimus ce-nocopiosus obtained from crabs in the mountainous area of south Anhui, were compared with those of Paragonimus westermani from the same batch of crabs.The metacercariae of E. cenocopiosus prefer the location in heart rather than in the muscle of crabs. The average infection rate of crabs was 42.8% by E. cenocopiosus, while that by P. westermani was 100%. The infection rate of rats with E. cenocopiosus was 3/6 and 10/10 with P. westermani and the recovery rate of worm of these two species was 4.5-8.8% and 22-72.2% respectively. The number and pattern of protein bands of metacercaria of these two species by disc-PAGE and IEF technics were different.

A DS-ELISA using rabbit anti-metacercaria serum-immunoglobulins as the first antibody and anti-metacercaria monoclonal antibodies as the second antibody were developed for the determination of circulating antigens in 38 cases of human paragonimiasis. The positive rate was 100% (38/38).

[Purpose] To summarize SHEN Yiping's clinical experience and academic views about treatment of elderly acute myeloid leukemia(AML) patients with traditional Chinese medicine(TCM).[Method] By means of learning form Professor SHEN in the clinic,collect cases and analyze them,and then conclude his clinical thought and dialectical methods,further summarize his clinical experience and academic views.[Result] Professor SHEN Yiping believes that these patients have deficiency of the lung,spleen and kidney,easily form the unhealthy environmental influences with shapes such as phlegm,damoeness,blood stasis,meeting with invaded by the invasion,causing the results of the happening of the disease.The functions of Zang-fu are damaged after chemotherapy,with the presentation of deficiency of qi and yin.Professor SHEN thinks that the treatment should be tonifying lung,spleen and kidney,dispelling the pathological products such as phlegm,dampness,blood stasis,eliminating the invasion at the same time.For those patients who are in remission,we should use the therapy of purging fire and maintaining yin,in order to remove leukemia residual of lesions thoroughly.Professor SHEN uses the anticancer drugs which could clear away heat and toxic frequently in order to depress the proliferation of the leukemia cells.[Conclusion]Professor SHEN is well-versed in the learning of both TCM and modern medicine,abundant clinical experience and rigorous dialectical methods,skilled in using TCM to treat elderly AML patients.He also has peculiar methods for the treatment of patients with recurrence of leukemia.His clinical experience and academic thoughts are worth learning and communication for us.

Next generation sequencing (NGS) based test provided us an unprecedented power for the molecular diagnosis of rare diseases with genetic etiology, thus enabling the practice of precision medicine.In order to maximize the clinical utilities of NGS-based test, attention should be paid to the improvement of the sequencing technology, data quality and the accuracy of data interpretation, as well as the clinical correlations and pre-and post-test genetic counseling services.In doing so, it is believed practice of NGS-based test can bring expected benefit to many patients and families.

Objective]To study the discipline of Guizhi Tang in clinical application, understand further indication of Guizhi Tang to improve clinical efficacy. [Method]After consulting medical cases about Guizhi Tang that are from different medical practitioners, analysis on the discipline of Guizhi Tang in clinical application with the help of six meridian syndrome differentiation, zang-fu differentiation and drug efficacy of Guizhi Tang. [Result]Guizhi Tang can be used to cure urticaria, coronary heart disease, nape carbuncle, high fever, half sweating and diarrhea. The cases of urticaria, diarrhea and nape carbuncle all contain symptoms of tai yang disease.The case of high fever is due to discord of ying-wei. The case of half sweating is due to discord of yin-yang. The case of coronary heart disease reflects Guizhi Tang can regulate spleen-stomach. [Conclusion] The medical cases about Guizhi Tang involve the departments of internal medicine, surgery, gynaecology and pediatrics,such as urticaria, coronary heart disease and so on. Using Guizhi Tang should get help from six meridian syndrome differentiation or zang-fu differentiation. When using six meridian syndrome differentiation, doctors should pay attention to difference of tai yang disease and other five meridian diseases. When using zang-fu differentiation, doctors should attach importance to the feature of Guizhi Tang that involves regulating yin-yang, spleen-stomach and ying-wei.

Objective] To investigate the relationship between pain from malnutrition and MM, looking for a cure for MM.[Methods]To analyze the relationship between pain from malnutrition and MM in clinical TCM based on the study of Neijing and the textual works of famous Chinese physicians. According to the etiology and pathogenesis to find the treatment method, and validation from clinical cases. [Result]We find that the pain from malnutrition caused by deficiency of kidney and spleen is a major cause of MM by summarizing of the ancient physicians. And we tally up the invigorating kidney is an important method for the treatment of MM.[Conclusion]Pain from malnutrition is an important pathogenesis of MM. To a method of treating MM, invigorating kidney and spleen is obvious and this method for the treatment of MM will bring more help.

The soybean high branched-chain amino acid mixture was composed of soybean protein hydrolyzed by special method with a few types additional amino acids in small amount. The mixture contained 68.9% of essential ami-no acids and 42.9% of branched-chain amino acids.The animal experiment showed that the mixture could promote the growth of young rats and maintain the normal levels of Hb, serum total protein and albumin. Its protein efficiency ratio (PER), true protein digestibility (TD), biological value (BV) and net protein utilization (NPU) were 2.78, 94.0%, 85.0 and 80.0% respectively. These values were significantly higher than that of casein. The results indicated that the soybean high branched-chain amio acid mixture did not produce amino acid antagonism caused by the high amount of branched-chain amino acids and had higher nutritional quality.

Objective To evaluate relations between lung cancer and the expressions of P53 protein together with proliferating cell nuclear antigen(PCNA) in specimens of lung lesions by needle biopsy.Methods CT-guidance percutaneous biopsy of lung lesions were performed in 66 patients with the determination of expressions of p53 protein and PCNA by flow cytometer (FCM). Results 1. The sensitivity of CT-guidance percutaneous biopsy was 94.3% in 53 cases of lung cancer with the diagnostic accuracy of 90.9% totally. The complication rate of pneumothorax was 4.6%. 2. The expression of P53 protein was (29.9?2.7)% in lung cancer (53 cases), while ( 17.9 ?2.8)% in benign lesions (13 cases) (t=2.0, P0.05).Conclusions FCM plays and valuable role in determining the expression of P53 protein and PCNA in the specimen of lung cancer by CT-guided percutaneous biopsy. The expression of p53 and PCNA may be useful in the diagnosis of lung cancer by providing the relation between imaging of lung cancer and the molecular mechanism, and furthermore revealing the characteristics of molecular biology of lung cancer at protein level.

The understanding and treatment of Traditional Chinese Medicine(TCM) on chronic idiopathic thrombocytopenic purpura(CITP) is prosperous,while no conclusion is yielded.By digging out documentation and summarizing clinical experience,"Qi and Yin Discrimination","Viscera Discrimination"and "Meridians Discrimination"are concluded to formulate the idea that the treatment of CIPT should bebased on Qi and Yin in this article,which aims at identifying the understanding of TCM on CITP,and then guiding clinical practice to improve therapeutic effect.

Background and purpose:The radio-labeled glucose analogue F-18-fluoro-2-deoxyglucose(FDG) is the most widely used tracer in PET imaging,and its application in oncology has become one of the standard imaging modalities.But FDG uptake is not tumor specifi c.The aim of this paper was to study the endoscopic result with the patients of 18F-FDG accumulations in gastrointestinal tract after PET-CT examinations.Methods:Thirtythree patients with 18F-FDG accumulations in gastrointestinal tract were correlated with endoscopic and histopathologic results.Results:Of these,14 patients were harboring newly occurring cancers in gastrointestinal tract(esophagus cancer,2 patients;gastric cancer,5 patients;colorectal cancer,7 patients) .Eight patients were identified with precancerous lesions(adenoma,4 patients;hyperplastic polyp,1 patient;Barrett’s esophagus,1 patient;intestinal metaplasia of the gastric mucosa,2 patients) .Inflammatory lesions were detected in six patients(active colitis,5 patients;anastomotic leakage,1 patient) .In fi ve patients,PET/CT showed normal fi ndings in endoscopic examinations.The false positive rate of PET-CT was 33.33%(11/33) .Conclusion:Endoscopic result is important for patients of 18F-FDG accumulations in gastrointestinal patient.