The tumor stem cell has attracted more and more attentions in recent years,which is involved in a possibili- ty that the knowledge on the tumor stem cell would be a novel base for the developing of research fields in the tumor pre- vent,diagnose and therapy.However,the basic knowledge system on tumor stem cell has not formed as an enough re- search about it has not been done.In this commentary,the author presents his understanding on several biological issues of tumor stem cells,and some controversy in this field as well.The author also wishes his necessary information would in- duce reader's interesting and future considerations.

A computer-based online search of PubMed database and Chinese Journal Full-text Database was undertaken to identify articles about the monitoring of pulmonary arterial pressure (PAP),mixed venous oxygen saturation (SvO2),and breathing mechanics in the patients undergoing lung transplantation from January 1990 to May 2008 to evaluate their clinical values in maintaining cardiorespiratory function of patients during anesthetic stage. PAP,cardiac output and SvO2 are key parameters of monitoring during the diseased lung resection and donor lung transplantation; breathing mechanics and SvO2 are key parameters of monitoring after lung ventilation. The former are reliable indicator to predict lung transplantation reaction and pulmonary edema and the latter can guide regulation of positive end expiratory pressure and when to remove extracorporeal membrane oxygenator. A combination of hemodynamics (PAP,pulmonary capillary wedge pressure,cardiac output,and central venous pressure),breathing mechanics and SvO2 consists an overall monitoring of heart and lung function. By Meta-analysis,these indicators can guide the regulation and protection of heart and lung function in patients undergoing lung transplantation and other severe patients during the anesthesia period.

Genetically engineered mice is one of the powerful research systems in exploring the gene protein life phenomenon,which is useful in generating the laboratory animal model of human diseases and developing the new drugs. With the development of functional genomics and model organism in life science area, biological medicine research is in need of genetically engineered mice deadly. There are still some problems to be resolved, including death effect, no or low expression and no phenotype. It is essential to improve the basic research related to genetically engineered mice.

Taking 175 township public hospitals in Jiangxi as the samples,descriptive statistics and comparative analysis were applied based on their financial yearly report data from 2012 to 2016 to analyze the operation situation of hospitals before and after the public reform,analyze the influences of medical reform policies on township public hospitals and propose relevant policies,so as to promote the soon coming urban public hospital reform in Jiangxi.

Objective: To improve the micro-manipulation of mouse fertilized eggs,promoting its survival rate and productivity in pseudo-pregnant mice. Methods: DNA fragments of definite concentration and size were used for micro-manipulation with conventional and improved methods, then their survival rate of manupulated eggs and productivity of pseudo-pregnant mice were analyzed and compared. Results: With improved methods, the survival rate of eggs increased from 60.3% to 79.5%, the productivity of pseudo-pregnant mice and offspring increased from 35.7% to 56.8% and 10.1% to 14.1% respectively. Conclusion: The improved methods of micro-manipulation can be used in transgenic mouse production.

Objective To compare the clinical effect of remifentanil and dexmedetomidine on controlled hypotension during endoscopic sinus surgery. Methods Forty patients undergoing endoscopic sinus surgery were randomly divided into group R and group D, 20 in each group. All patients received controlled hypotension when the surgeons began to sterilize the nasal cavity for the purpose of maintaining the MAP at 60 ~ 70 mmHg. Group R received remifentanil 1μg/kg over 1 minute, followed by 0.2 to 0.4μg/kg per minute infusion during maintenance, whereas group D received dexmedetomidine 1μg/kg over 10 minutes, followed by 0.2 to 0.7μg/kg per hour during maintenance. At the moment of pre-induction(T0), incision(T1), 30 min(T2) and 60 min(T3) after incision and 10 min(T4) after end of controlled hypotension, MAP and HR were measured. Quality of the surgical field, blood loss and extubation time were recorded. Sedation and postoperative pain were assessed on arrival at the PACU. Results The goals for the MAP level were achieved and the surgical field quality was ideal in both groups. The MAP and HR values at T1~ T3 were significantly lower than those at T0 in both groups (P < 0.05). There were no significant differences in the MAP or HR values between the groups at each time point (P>0.05). No significant difference was founded between the groups in the Fromme operative field score and blood loss (P > 0.05). The extubation time was significantly shorter in group R than in group D(P<0.05). The sedation score in the PACU was significantly lower in group R than in group D(P<0.05), while there were no significant differences in postoperative pain between the groups(P<0.05). Conclusion Both remifentanil and dexmedetomidine are safe agents for controlled hypotension and are effective in providing satisfactory surgical fields during endoscopic sinus surgery. However , remifentanil is advantageous in time for extubation and complete consciousness from anesthesia and therefore it may have the advantage of fast track anesthesia.

Objective To observe the clinical effect of the transdermal acupoint stimulation treatment on intractable hiccups of patient with blood disease. [Methods]The 57 inpatients with intractable hiccups induced by chemotherapy or corticosteroids were randomly divided into two groups, percutaneous acupoint electric stimulation group and control group with Ritalin injecting in zusani, from January 2010 to December 2013. Clincal effect and adverse reaction were compared and analyzed after treatment. [Result]There was no significant difference on the clinical effect between two groups,while the adverse reaction in percutaneous acupoint electric stimulation therapy group was relatively mild.[Conclusion] Percutaneous acupoint electric stimulation is an effective method for the treatment of intractable hiccups, which should be popularized in clinical practice.

Objective To investigate the expression of vascular growth factors including vascular endothelial growth factor(VEGF), basic fibroblast growth factor(bFGF) and platelet-derived growth factor (p-DGF) in placentae of pregnancy-induced hypertension(PIH) patients and their significance. Methods Placental tissues of 15 normal gravidas and 19 PIH women were collected. The expression of vascular growth factors were detected by immunohistochemistry (PAP) combined with the analysis of computer microphotography. Results The average grey value difference (GVD) of VEGF was 61.6?3.7 in moderate and severe PIH group, which was significantly lower than that of the normal control group (71.1?5.8) and mild PIH (70.9?8.9, P

Objective To investigate the effects of patient-controlled intravenous analgesia (PCIA) with lornoxicam and fentanyl on arrhythmia and the expression of platelet membrane glycoproteins in patients with coronary artery disease (CAD) after abdominal surgery.Methods Eighty ASA II or III patients with CAD aged 51-66 yrs weighing 59-68 kg presenting for bdominal surgery participated in this study. CAD was diagnosed by clinical symptoms and ischemic changes on ECG. The patients were premedicated with intramuscular henobarbital 0.1 g and scopolamine 0.3 mg. Anesthesia was induced with fentanyl, droperidol, propofol and vecuronium and maintained with propofol, fentanyl and vecuronium. The patients received PCIA after operation. The PCIA solution contained fentanyl 0.9 mg and droperidol 5 mg in 100 ml of normal saline (N.S. ) in group A ( n - 40) or lomoxicam 56 mg, fentanyl 0.2 mg and dropendol 5 mg in 100 ml N.S. in group B ( n = 40). In group A the loading dose was fentanyl 0.05 mg and in group B lornoxicam 4 mg. PCIA included a background infusion at 2 ml ? h-1 and a bolus of 0.5 ml with a 15 min lock-out. VAS (0 = no pain, 10 = worst pain) was used to measure pain intensity In addition to BP, HR and SpO2 monitoring ECG was continuously monitored with a Holler monitor after operation Blood samples were taken from peripheral vein before and 6h after operation and on the 1st, 2nd, 7th and 8th postoperative days for determination of the expression of CD62p, CD63 and CD41/CD61 on the platelet membrane, platelet count, prothrombin time ( PT) thrombin time (TT) and partial thromboplastin time (PTT) Results The two groups were comparable with respect to sex, age, body weight, severity of CAD, duration of operation and intraoperative blood loss. The patients received no blood transfusion during operation. There was no significant difference in VAS score, platelet count, PT, TT and PIT between the two groups. The incidence of atrial and ventricular premature beat on ECG and the expression of CD41 /CD61 , CD62P and CD63 on the platelet membrane were significandy lower in group B than in group A on the 7th and 8th postoperative days (P

At present improper use of antimicrobial agents in China is rather prevalent. For this reason we started from January 2000 to take general management methods and corresponding intervention measures with regard to the use of antimicrobial agents. We then made a survey of the use of antimicrobial agents before and after the adoption of the above methods so as to evaluate the effect of the intervention measures.