1.The optimization of portal venous system imaging with 6 4-slice spiral CT
Yindi ZHU ; Yinyin CHEN ; Liang GUO
Journal of Practical Radiology 2014;(4):656-659
Objective To compare the image quality of portal venous system in the patients with live cirrhosis and portal hyper-tension using two different inj ection methods,so as to investigate the optimization of imaging techniques.Methods Single-phase in-jection (group A)or biphasic injection (group B)was applied by random on 40 patients for portal venous system vascular imaging, and subsequently image quality and technical advantages between the two groups were compared and analyzed respectively.Results 1.There was no significant difference in image quality between the two groups(P>0.05),while contrast agent dosage (90 mL)in group B is less than group A(100 mL).2.The CT value of Portal vein,liver parenchyma and P-L discrepancy in group B were slightly lower than those in group A,however,no statistical difference was noted(P>0.05).After 45 second,the P-L discrepancy of group B was larger than that of group A.The peak times of portal vein,liver parenchyma and the P-L discrepancy in group B were longer than those in group A(P<0.05).3.The graph of P-L discrepancy demonstrated:the threshold interval of P-L discrep-ancy in group B was longer than that in group A.Conclusion Biphasic inj ection methods could not only afford reliable imaging quali-ty on portal venous system vascular imaging in the patients with liver cirrhosis and portal hypertension,but also lead to a less total dose of contrast agent.Therefore,it could be used as an optimal choice of imaging technology for portal venous system vascular ima-ging.
2.Mechanism of RING finger protein 34 involved in innate immunity:a preliminary study
Yongjie ZHU ; Pingping ZHANG ; Pengyu ZHOU ; Penghao WANG ; Jiankang CHEN ; Yinyin TIAN ; Xiang HE ; Hui ZHONG
Military Medical Sciences 2017;41(1):43-47,61
Objective To study the mechanism of RING finger protein 34 ( RNF34 ) involved in innate immunity . Methods Recombinant PCR was used and transient expression of the plasmid was achieved in HEK 293T cells.The cells were stimulated with Sendai virus ( SeV) or N-RIG-Ⅰfor the indicated time while luciferase activity was observed using the dual-luciferase reporter assay kit .Results We constructed the plasmid pcDNA 3-Flag-RNF34 and its three mutations .The study found that when stimulated by SeV , RNF34 could inhibit the activity of NF-κB and IFN-βmore significantly than RNF34-ΔFYVE, RNF34-ΔCID and RNF34-ΔRING.We also found that RNF 34 and its three mutants had similar inhibitory effect when the activation of NF-κB and IFN-βwas stimulated by the N-RIG-Ⅰ.Conclusion RNF34 negatively regulates innate immunity by acting on the RIG-Ⅰ-MAVS signaling pathway .
3.Therapeutic effect of endovascular embolization for perimedullary arteriovenous fistula
Hao HE ; Zhen XU ; Yingge XU ; Song ZHANG ; Ming ZHU ; Lei YAN ; Yinyin WU ; Shaofeng SHUI
Chinese Journal of Interventional Imaging and Therapy 2023;20(12):726-729
Objective To observe the therapeutic effect of endovascular embolization for perimedullary arteriovenous fistula(PMAVF).Methods Data of 8 PMAVF patients who underwent endovascular embolization were retrospectively analyzed.Findings of digital subtraction angiography(DSA)immediately and 6 months after treatment were reviewed,and the modified Aminoff-Logue score(mALS)was compared before and 6 months after treatment.Results Among 8 cases of PMAVF,there were 2 cases of type Ⅰ,5 cases of type Ⅱand 1 case of type Ⅲ,among them 3 were treated with Onyx glue while 5 with Glubrane glue.The immediate DSA results after treatment showed complete embolization of the fistula in all 8 cases,while those 6 months after treatment showed no recurrence.One case recovered and the other 7 cases improved,and mALS(2.25±2.12)6 months after treatment was lower than that(5.50±2.39)before treatment(P<0.05).Conclusion Endovascular embolization could safely and effectively treat PMAVF.
4.3-Methyladenine ameliorates CCl4-induced liver fibrosis and gut microbiota in mice
Yinyin FAN ; Bingying WANG ; Hong ZHANG ; Xueming ZHU ; Hong DU
Chinese Journal of Clinical Laboratory Science 2019;37(7):546-549
Objective:
To investigate the role of gut microbiota in the amelioration of liver fibrosis induced by CCl4 in mice by 3-methyladenine (3-MA).
Methods:
Fifteen mice were randomly divided into normal control group, liver fibrosis group and 3-MA treatment group. The liver fibrosis model was established by injecting CCl4, and the mice in the 3-MA treatment group were injected 3-MA additionally from the third week onwards. After 8 weeks, all of the mice were sacrificed and their blood, liver tissue and fecal samples were collected to analyze serum ALT, AST, GGT levels, liver histopathology and gut microbiota.
Results:
Compared with the liver fibrosis group, serum ALT and AST levels in 3-MA treatment group decreased obviously ([68.6±4.2] U/L vs [111.0±7.8] U/L, [179.0±12.9] U/L vs [253.2±26.7] U/L, P<0.01), and the degree of hepatic histopathological changes was reduced. The intestinal flora in three groups were distinguished by principal coordinate analysis (PCoA) and non-metric multi-dimensional scaling (NMDS) analysis. Compared with the normal control group, there were decreased Alpha diversity of intestinal community, reduced significantly abundance of Lachnospiraceae, and increased obviously abundance of Actinobacteria and Desulfovibrionacea in the liver fibrosis group (P<0.05). Compared with the liver fibrosis group, there were increased Alpha diversity of intestinal community, increased significantly abundance of Lachnospiraceae and Blautia, and reduced abundance of Bifidobacteriaceae in the 3-MA treatment group (P<0.05). In addition, the abundance of Lactobacillaceae in the 3-MA treatment group was significantly higher than that in the normal control group (P<0.05).
Conclusion
3-MA improves the liver fibrosis of mice induced by CCl4, and gut microbiota may play an active role in this process.
5.Study on Protective Effects of Crocin against Triptolide-induced Visceral Organ Injury in Mice
Yinyin YAN ; Min YAN ; Xiangxiang WU ; Xin ZHU ; Wenbo SHI ; Mengyuan JIANG ; Huahui ZENG
China Pharmacy 2021;32(19):2320-2326
OBJECTIVE:To study the prot ective effects of crocin (CR)against triptolide (TP)-induced visceral organ injury in mice,and to provide reference for the studying TP compatibility and detoxification. METHODS :Fifty mice were randomly divided into normal group ,TP low-dose and high-dose groups (i.e. TP-L group ,TP-H group ,with 300,600 μg/kg),TP low-dose and high dose combined with CR groups (i.e. TP-L+CR group ,TP-H+CR group ,with 300 μg/kg TP+100 mg/kg CR ,600 μg/kg TP+ 100 mg/kg CR ),with 10 mice in each group. Except for normal group ,other groups were given relevant medicine intragastrically , once a day ,for consecutive 7 d. The body weight of mice was weighted every day ,and their death was recorded. After last administration,the mice were sacrificed ,and the heart ,liver,kidney and testis were taken ,and the organ index was calculated ; serum levels of ALT ,AST,BUN and Scr ,the activity of T-SOD and the contents of MDA were all determined. The pathological changes of heart ,liver,kidney and testis were observed ;mRNA expression of Bcl- 2,Bax and caspase- 3 in liver tissue were determined. RESULTS :Three,five,two and three mice in TP-L group ,TP-H group ,TP-L+CR group and TP-H+CR group died respectively,and the survival rates were 70%,50%,80% and 70%,respectively. Compared with normal group ,the body weight (7th day of experiment ),heart index ,liver index ,kidney index (except for TP-L group ),testicular index ,T-SOD activity and mRNA expression of Bcl- 2 in liver tissue ,serum levels of ALT (except for TP-L group ),AST(except for TP-L group ),BUN and Scr,MDA content and mRNA expression of Bax ,mRNA expression of caspase- 3 in liver tissue were increased significantly (P< 0.05 or P<0.01). There were obvious pathological changes in heart ,liver,kidney and testis tissue. Compared with the same dose of TP alone group ,the above indexes of TP combined with CR group were improved in varying degrees. Except for the renal index and serum ALT level of TP-L+CR group ,there was statistical significance for all indexes (P<0.05 or P<0.01);the pathological injuries of heart ,liver,kidney and testis were significantly improved. CONCLUSIONS :CR can relieve the damage of heart , liver,kidney and testis induced by TP ,which may be related to the antioxidant stress of CR.
6.Establishment of whole-process intelligent pharmaceutical care model for peritoneal dialysis patients
Yongfu HANG ; Yan XU ; Xiaohua DAI ; Tiantian WU ; Yinyin DUAN ; Deyu XU ; Kun HU ; Xingxing LIU ; Jianguo ZHU ; Liyan MIAO ; Lin LI
China Pharmacy 2023;34(21):2644-2648
OBJECTIVE To develop a whole-process intelligent model of pharmaceutical care for peritoneal dialysis (PD) patients, and to provide a reference for clinical pharmacists to provide standardized PD pharmaceutical care. METHODS The pharmaceutical care mode of PD patients at home and abroad was investigated and analyzed. Based on the actual situation of the First Affiliated Hospital of Soochow University (hereinafter referred to as “our hospital”), with “home→PD center outpatient→ inpatient department” as the main node, the recycling process of medication reconciliation was optimized. The whole-process intelligent pharmaceutical care model of PD was illustrated by improving the Chinese version of the drug-related problems (DRPs) classification tool, developing the corresponding pharmaceutical care process, and presenting specific cases. RESULTS Based on the medication therapy management (MTM) platform, our hospital had built a closed-loop PD whole-process intelligent pharmaceutical care model of “in-hospital pharmaceutical care (building document)-PD outpatient MTM-home pharmaceutical care (online App management)”. A “double cycle” workflow of “admission→discharge→outpatient” medication reconciliation cycle and “discovery-analysis-intervention-follow-up-record-evaluation” DRPs cycle was formed. CONCLUSIONS The establishment of the whole-process intelligent pharmaceutical care model for PD in our hospital provides experience for standardizing pharmaceutical care for PD patients, and can reduce DRPs.
8.A systematic survey of LU domain-containing proteins reveals a novel human gene, LY6A, which encodes the candidate ortholog of mouse Ly-6A/Sca-1 and is aberrantly expressed in pituitary tumors.
Dan LIU ; Chunhui XU ; Yanting LIU ; Wen OUYANG ; Shaojian LIN ; Aining XU ; Yuanliang ZHANG ; Yinyin XIE ; Qiuhua HUANG ; Weili ZHAO ; Zhu CHEN ; Lan WANG ; Saijuan CHEN ; Jinyan HUANG ; Zhe Bao WU ; Xiaojian SUN
Frontiers of Medicine 2023;17(3):458-475
The Ly-6 and uPAR (LU) domain-containing proteins represent a large family of cell-surface markers. In particular, mouse Ly-6A/Sca-1 is a widely used marker for various stem cells; however, its human ortholog is missing. In this study, based on a systematic survey and comparative genomic study of mouse and human LU domain-containing proteins, we identified a previously unannotated human gene encoding the candidate ortholog of mouse Ly-6A/Sca-1. This gene, hereby named LY6A, reversely overlaps with a lncRNA gene in the majority of exonic sequences. We found that LY6A is aberrantly expressed in pituitary tumors, but not in normal pituitary tissues, and may contribute to tumorigenesis. Similar to mouse Ly-6A/Sca-1, human LY6A is also upregulated by interferon, suggesting a conserved transcriptional regulatory mechanism between humans and mice. We cloned the full-length LY6A cDNA, whose encoded protein sequence, domain architecture, and exon-intron structures are all well conserved with mouse Ly-6A/Sca-1. Ectopic expression of the LY6A protein in cells demonstrates that it acts the same as mouse Ly-6A/Sca-1 in their processing and glycosylphosphatidylinositol anchoring to the cell membrane. Collectively, these studies unveil a novel human gene encoding a candidate biomarker and provide an interesting model gene for studying gene regulatory and evolutionary mechanisms.
Humans
;
Membrane Proteins/genetics*
;
Pituitary Neoplasms/genetics*
;
Biomarkers