Objective To evaluate the intervention effects of systemic rehabilitation training on postoperative disuse shoulder dysfunction in hand injury patients received abdominal pedicled skin flap transplantation. Methods The 92 patients received same operation of abdominal pedicled skin flap surgery for unilateral hand in Hand and Foot Surgery Department of Provincial Hospital Affiliated to Shandong University and were divided into two groups, in which 45 patients as control group from March to April 2014 and 47 patients as experimental group from June to July 2014. The patients of control group received conventional therapy and nursing, while the ones of experimental group received systematic rehabilitation training by nurses and doctors before, during and after operation. Shoulder function was scored at the day 1 and day 7 after the pedicle division operation according to the shoulder joint function assessment scale, and we employed SPSS 20. 0 to analyze the results. Results The shoulder function scores of experimental group patients were (72. 96 ± 4. 72) and (87. 91 ± 4. 74) at the day 1 and day 7 after the pedicle division operation, which were better than the scores of control group patients, [(65. 11 ± 6. 74) and (79. 38 ± 7. 31)] (t =6. 492,6. 677;P <0. 01). Conclusions The systemic rehabilitation training program, which requirements were definite, and guidance was standard and easy to perform, can significantly improve the shoulder function of the patients received abdominal pedicled skin flap transplantation.