A novel method was proposed for the quantification of nitrite ion in aqueous samples by the combination of an advanced quantitative fluorescence model with ratiometric fluorescence indicator 2 , 3-diaminonaphthalene . The proposed method was tentatively applied to direct quantitative determination of nitrite ion in turbid environmental water samples containing both scatterers and absorbers; and its performance was thoroughly investigated and evaluated. Experimental results showed that the proposed method could realize accurate quantitative determination of nitrite ion in environmental water samples with recovery rates in the range of 90. 8%-103. 0%, which were comparable to the corresponding values of HPLC-DAD experiments. The limit of detection and limit of quantification of the proposed method were estimated to be 1 . 9 and 5. 8 nmo/L, respectively.

Objective To explore the relationship between metabolic syndrome (MS) and prostate volume. Methods One thousand and ninety-eight males aged 36-90 years were divided into two groups according to the definition of MS by CDS in 2004 : MS group and control group. The blood pressure, body weight and height were measured, and the body mass index (BMI) was calculated. Biochemical assays including fasting plasma glucose, total cholesterol, triglycerides, low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), and prostate specific antigen (PSA) were performed. Prostate volume was measured by abdominal ultrasound. Results (1) The BMI, systolic blood pressure, diastolic blood pressure, fasting plasma glucose, serum triglyceride, total cholesterol, prostate volume and PSA level were higher, while HDL-C level was lower in MS group than in control group (all P<0.05). (2)Compared with control group, the prostate volume was enlarged in MS group [(37.8±21.3)ml vs. (31.0±11.1)ml, P<0.01]. (3)The prostate volume was positively correlated with PSA level (r= 0. 350, P< 0. 01), age (r = 0. 429, P<0.01), BMI (r=0.145,P<0.01) and systolic blood pressure (r=0.133, P<0.05), and was negatively correlated with diastolic blood pressure (r= -0. 193,P<0. 01). ConclusionsMetabolic syndrome is related to the enlargement of prostate volume.

Objective To investigate the effect of real-time continuous monitoring system(RT-CGMS)on serum inflammatory factors and prognosis in patients with sepsis.Methods According to the prospective studies,patients were randomly divided into two groups Patients with sepsis were randomly divided into real-time continuous monitoring of blood glucose group and monitoring blood glucose meter group,of which the fasting serum tumor necrosis factor alpha(TNF-α) and interleukin-6(IL-6) and high sensitive C reactive protein (hs-CRP) were detected on the 1st,3rd,7th day.Meanwhile,insulin dosage,incidence of hypoglycemia and mortality rate of 28 days of patients in two groups were recorded,and the above indexes were analyzed.Results TNF-α,IL-6 and hs-CRP in the real-time continuous blood glucose monitoring group were (16.12±2.42)pg/ml、(112.37±17.39)pg/ml and(19.62±2.72)mg/L on the 7th day respectively,while TNF-α,IL-6 and hs-CRP in the blood glucose monitoring group were(23.46±3.12)pg/ml、(140.19±21.48)pg/ml and(25.42±2.54)mg/L on the 7th day,respectively.The decrease of TNF-α,IL-6 and hs-CRP in the continuous blood glucose monitoring group was more significant than that in the blood glucose monitoring group (P＜0.05).Insulin dosage in real-time continuous blood glucose monitoring group was (90.62±15.79)u,hypoglycemia rate was 5.4％,fatality rate was 10.81％ in 28 days of hospitalization,insulin dosage was (130.59±20.65)u,hypoglycemia rate was 22.85％,and fatality rate was 31.42％ in 28 days of hospitalization.There was significant difference between the two groups(P＜0.05).Conclusion For sepsis patients with stress hyperglycemia,a real-time continuous monitoring system combined with insulin therapy can improve the inflammatory response,and control blood glucose effectively as well as reduce mortality.