Objective To study the surgical treatment of venous leg ulcer. Methods The surgical treatment of venous leg ulcer in 101 cases from January 1980 to December 2002 in our hospital was retrospectively analyzed. Results Of 72 cases undergoing subfascial communicating vein ligation around the base of the ulcer, 52 cases were healed within 1 to 3 months, 12 cases within 4 to 5 months, 8 cases after skin graft, but 3 cases were complicated with postoperative infection and 2 cases suffered from relapse of ulcer. However, of the 12 cases undergoing peripheral transfixion of the ulcer through the skin, 2 cases were complicated with skin necrosis and infection, 10 cases healed within 6 months to 1 year. All the 11 cases undergoing endoscopic communicating vein dissection were healed without complications within 1～2 months. Conclusion The surgical procedures for venous leg ulcer should be performed to block the regurgitation of the communicating veins by means of lowering the venous leg pressure. Endoscopic communicating vein ligation or spiral dissection of the communicating vein is valuable in clinical application.

Objective To investigate the effects of in situ venous arterialization on extensive artery obliterans occlusion of the lower extremity. Methods Lumbar sympathetic ganglionectomy and one stage in situ arterialization of the great saphemous vein were performed in 104 ischemic limbs of 88 patients with extensive arterial occlusion. Results Eighty-two of 104 limbs were followed-up from 6 months to over 6 years. The intermittent claudication, night pain improved in all cases, with satisfactory wound healing and no swelling of the lower limbs. Conclusions Arterial blood flow through venous conduit improves and reconstructs the blood circulation of the ischemic limbs.

BACKGROUND AND PURPOSE: Encephalitis caused by Listeria monocytogenes (L. monocytogenes) is rare but sometimes fatal. Early diagnosis is difficult using routine cerebrospinal fluid (CSF) tests, while next-generation sequencing (NGS) is increasingly being used for the detection and characterization of pathogens. METHODS: This study set up and applied unbiased NGS to detect L. monocytogenes in CSF collected from three cases of clinically suspected listeria meningoencephalitis. RESULTS: Three cases of patients with acute/subacute meningoencephalitis are reported. Magnetic resonance imaging and blood cultures led to a suspected diagnosis of L. monocytogenes, while the CSF cultures were negative. Unbiased NGS of CSF identified and sequenced reads corresponding to L. monocytogenes in all three cases. CONCLUSIONS: This is the first report highlighting the feasibility of applying NGS of CSF as a diagnostic method for central nervous system (CNS) L. monocytogenes infection. Routine application of this technology in clinical microbiology will significantly improve diagnostic methods for CNS infectious diseases.
Central Nervous System ; Cerebrospinal Fluid ; Communicable Diseases ; Diagnosis ; Early Diagnosis ; Encephalitis ; Humans ; Listeria monocytogenes* ; Listeria* ; Magnetic Resonance Imaging ; Meningitis, Listeria ; Meningoencephalitis* ; Methods