Objective To identify the potential association of transcription factor 7-like 2(TCF7L2 polymorphisms with type 2 diabetes mellitus in Uygur population of Xinjiang region .Methods In this case-control study ,819 ca-ses of type 2 diabetes mellitus patients were recruited in case group and 731 healthy individuals were selected as control.5 mL of blood sample were collected from each subject .The polymorphism was examined by matrix-assis-ted laser desorption/ionization-time of flight ( MALDI-TOF) and the OR value (95%CI) was evaluated by Logistic Regression Method to analyze the relationship between susceptibility to type 2 diabetes mellitus and different geno-types.Results In case group, the frequencies of TC, CC genotype and C allele at rs7901695 were higher than the corresponding frequencies in control group (P<0.05).The interaction between TCF7L2 and environment risk factors did not contribute to the occurrence of the type 2 diabetes mellitus .Conclusions The polymorphisms of rs7091695 in TCF7L2 but not rs7085532 in TCG7L2 may be associated with type 2 diabetes mellitus in Uygur pop-ulation in Xinjiang region .

Objective To analyze the distribution characteristics of blood uric acid and its relationship with the risk factors of metabolic syndrome in Uygur.Methods The questionnaire,anthropometric measurements,and biochemical detection were carried out in 4 428 healthy Uygur subjects in Xinjiang Urumqi and Kashi hospitals.Results (1) The prevalences of hyperuricemia and metabolic syndrome were 21.3 ％ and 8.2％,respectively.With the increased blood uric acid level,the incidences of coronary heart disease,hyperglycemia,hypertension,central obesity,and dyslipidemia were raised.Blood pressure,blood glucose,HbA1c,triglyceride,total cholesterol,apolipoprotein A,low density lipoprotein-cholesterol,body mass index (BMI),and waist to hip ratio (WHR) were increased with increased uric acid level,while high density lipoprotein-cholesterol was decreased.(2) The incidence of hyperuricemia was increased further when the number of metabolic syndrome components was accumulated (P＜0.01).With the increase of uric acid level,the prevalence of metablic syndrome gradually raised (P＜0.01).(3) Multiple logistic regression analysis showed that WHR (OR =7.639,95 ％ CI 1.744-33.466),coronary heart disease (OR =2.784,95 ％ CI 1.718-4.510),hyperuricemia (OR =2.155,95 ％ CI 1.457-3.188),smoking (OR =1.437,95％ CI 1.071-1.927),family history of metabolic diseases (OR =1.333,95％ CI 1.044-1.703),occupational pressure (OR =1.290,95 ％ CI1.021-1.631),and BMI (OR =1.146,95 ％ CI 1.112-1.181) were the risk factors of metabolic syndrome.Exercise (OR=0.472,95％ CI0.370-0.604) and low salt diet (OR=0.793,95％ CI0.662-0.949) were the protective factors.Conclusion Serum uric acid level is correlated with a variety of metabolic parameters.With the increased uric acid level,the risk of multiple metabolic abnormality was increased.Comprehensive prevention and control should be taken for the reduction of the risk factors and much attention should be paid to the adverse effects of hyperuricemia.

The association between the polymorphism of SLC17A1 gene and hyperuricemia in Uyghur population in Xinjiang region was explored by Chi-square test.A total of 1 024 patients with hyperuricemia and 1 033 healthy volunteers were included.The genotype frequencies of CC,CT,and Tr in hyperuricemia and healthy controls were 24.9％,53.14％,21.96％ and 29.7％,47.52％,22.77％,respectively.There was statistical difference in SLC17A1 rs9467596 genotype frequencies between hyperuricemia cases and controls (x2 =7.492,P =0.024).CT genotype could increase the risk of hyperuricemia compared with CC genotype (OR =1.334,95％ CI 1.082-1.644).No statistical significance among the genotypes was found in age,body mass index,blood pressure,blood glucose,triglyceride,total cholesterol,High-density lipoprotein-cholesterol,low-density lipoprotein-Cholesterd,blood urea nitrogen,and Creatinine.The polymorphism of rs9467596 in SLC17A1 may be a genetic marker to assess risk of hyperuricemia among Xinjiang Uyghur population.

AIM To investigate the anticonvulsive action of supercritical CO2 ethanol extract from Pinellia Pedatisecta Schott(SEE-CO2PP). METHODS The rat convulsive model was induced by penicillin localized injected in rat cortex. The effects of SEE-CO2PP on the latency of seizure and changes of convulsive behaviors were investigated. The latency of epileptiform discharge, and frequency and amplitude of highest wave in cortex and hippocampus were recorded by using RM6240C multichannel physiological signal collection and analysis recorder. At the same time, the contents of glutamic acid (Glu), aspartic acid (Asp), glycine (Gly) and γ-aminobutyric acid (GABA) in hippocampus were determined with high performance liquid chromatography. RESULTS SEE-CO2PP 15 and 30 g·kg-1, ig, prolonged the latent period of seizure and weakened the extent. SEE-CO2PP also prolonged the latent period of epileptiform discharge, reduced the frequency and decreased amplitude of the highest wave in both cortex and hippocampus. Moreover, SEE-CO2PP increased the content of GABA in hippocampus, but the levels of Gly,Asp and Glu had no obvious changes. CONCLUSION SEE-CO2PP inhibits the epileptiform discharge and convulsive behaviors of convulsive model rats, which suggests SEE-CO2PP has anticonvulsive action.

Objective To investigate the relationship between glucokinase regulator protein ( GCKR) gene polymorphism rs780094 and hyperuricemia in Uygur in Xinjiang. Methods A case-control study including 1 026 patients with hyperuricemia and 1 030 normal subjects was conducted. All the subjects were genotyped for GCKR gene rs780094 by Sequenom MassARRAY system. The results of rs780094 genotype and allele frequency between hyperuricemia group and control group were compared. The associations of different genotypes of rs780094 with blood pressure, blood lipid, and blood glucose were analyzed. Logistic regression analysis was used to analyse the relationship between polymorphism of rs780094 and hyperuricemia in Uygur in Xinjiang. Results The distributions of three genotypes(G/G, A/G, A/A) and two allele frequency (G and A) in GCKR rs780094 revealed statistical difference ( P<0. 05 ) between hyperuricemia group and control group. A tendency toward association with hyperuricemia was observed under dominant model(OR=1. 295, 95%CI 1. 078~1. 554,P=0. 006) and recessive model(OR=1. 284, 95% CI 1. 024 ~1. 611,P=0. 030). The levels of systolic blood pressure, diastolic blood pressure, and total cholesterol were lower in hyperuricemia group with GCKR gene rs780094 loci GG genotype than those with AA+AG genotype. After adjusting confounding factors which had significant difference in the single factor analysis, logistic regression analysis showed that rs780094 A/A and A/G might be risk factors of hyperuricemia in Uygur in Xinjiang (OR=1. 355,95% CI 1. 094 ~1. 679,P=0. 005). Conclusion The GCKR rs780094 is associated with hyperuricemia in Uygur in Xinjiang. The A/A and A/G genotype of the GCKR rs780094 may increase the risk of hyperuricemia.

Objective The whole process of vaccine preparation is time-consuming and technically challenging. Here the hGM-CSF-engineered K-562 cell line was constructed to simplify tumor vaccine preparation process. Methods The eukaryocyte expressing plasmid pcDNA3.1/GM-CSF was first constructed and its accuracy was verified through sequencing. The pcDNA3.1/GM-CSF was transfected into COS-7 cells to verify GM-CSF expression and cytokine activity using TF-1 cell line. Then the plasmid was transfected into K-562 cell line using liposome method, and was selected under G-418 and sub-cloned by limiting dilution. GM-CSF product from the monoclone GM-CSF-K-562 cell lines was quantified using ELISA method. Results We successfully constructed the hGM-CSF eukaryocyte expressing plasmid and hGM-CSF expressing K-562 cell line. Conclusion The construction of K-562/GM-CSF line will simplify the preparation of tumor vaccine, thus facilitating the application of tumor vaccination therapy in clinical application.

Objective To investigate the expression level and clinical significance of WT1 gene in acute luekemia (AL) patients.Methods WT1 gene level was detected by real time quantitative-polymerase chain reaction in acute myelogenous leukemia (AML) and in acute lymphocytic leukemia (ALL) patients.Then the expression levels of WT1 gene in different subtypes of AML were compared,and the correlation between gene expression and disease courses and prognosis were observed.Moreover,the relationship between disease courses and WT1 expression in patiens after receiving haemopoietic stem cell transplantation were analyzed.Results Among 66 cases,WT1 expression positive rate was 87.5 ％ (14/16) in AML and 76.0 ％ (38/50) in ALL.In AML,the expression level in M3 showed the lowest than that in any other subtypes (compared with M1,M2,M4,M5,P value was 0.040,0.007,0.006 and 0.01,respectively).The expression level of WT1 was closely correlated with leukemia disease courses.The expression level in complete remission (CR) group showed a significant lower expression level than that in non-remission group (P =0.018) and relapse group (P =0.003),and the re-increase of WT1 expression level could predict relapse as early as 1.5 months.Moreover,WT1 expression also showed an close relationship with prognosis of patients receiving haemopoietic stem cell transplantation.Patients whose WT1 was undetectable had a better prognosis than those with persistent expression,and increase again after becoming undetectable.Conclusion WT1 has a high expression level in AL,which can represent minimal residual disease.The expression level in M3 was lowest than that in different AML subtypes,and its expression level has a close correlation with clinical disease course and prognosis of AL.

Objective To study the clinic effect and safety of MEAD chemotherapy regimen for adult patients with relapsed or refractory acute lymphocyte leukemia. Methods Between July 2006 and July 2009,twenty-two adult patients with relapsed or refractory acute lymphocyte leukemia received MEAD regimen (mitoxantrone 6 mg/d dl-3 iv drip,cytarabine 100 mg/d dl-5 iv drip,etoposide 100 mg/d dl-5 iv drip,dexmethasone 10 mg/d dl-8 iv drip). Results The complete remission (CR) rate of adult patients with relapsed or refractory acute lymphocyte leukemia was 31.8 %,the partial remission(PR) rate was 22.7 % and the overall response (OR) rate 54.5 %. The cumulitive CR rate was 50.0 %,and the PR rate 40.9 % after two times MEAD chemotherapy regimen. The main adverse effect was different level of myelosuppression,and other toxicity of vital organ was mild. Conclusion MEAD regimen is effective and can be tolerated for adult patients with relapsed or refractory acute lymphocyte leukemia,and its side effect is mild.

Objective To investigate the correlation between TCF7L2 gene rs3814570 polymorphisms with type 2 diabetes mellitus(T2DM) in Uygur population of Xinjiang area.Methods By adopting the case-control study design,949 cases of T2DM were recruited as the observation group and 963 individuals Undergoing healthy physical examination were selected as the control group.The TCF7L2 gene polymorphism was detected by matrix-assisted laser desorption/ionization-time of flight(MALDI-TOF).Results The statistical differences in frequencies of CC,CT and TT genotypes and the C and T allele frequencies on TCF7L2 rs3814570 were found between the T2DM group and control group(P＜0.05).The risk of suffering from T2DM in the carriers of CT genotype was 0.331 times of that in the carriers of CC genotype(OR =0.331,95 ％ CI:0.166-0.661,P =0.002),the risk of suffering from T2DM in the carriers of TT genotype was 0.539 times of that in the carriers of CC genotype(OR=0.539,95％CI:0.348-0.834,P=0.005),and the risk of suffering from T2DM in the carriers of T allele was 0.501 times of that in the carriers of C allele(OR=0.501,95 ％ CI:0.377-0.664,P＜ 0.01).Among all subjects,the FPG level of the CT + TT genotype group on TCF7L2 gene rs3814570 locus was significantly lower than that of the CC genotype group(P＜0.05).Conclusion The rs3814570 locus in TCF7L2 gene may be associated with T2DM occurrence in Uygur population of Xinjiang area,the T allele and TT genotype might be protective factors of T2DM.

AIM: To investigate whether rs7903146 polymorphisms in transcription factor 7-like 2 (TCF7L2) gene are associated with susceptibility to type 2 diabetes mellitus (T2DM) in Chinese Uygur population.METHODS: In this case-control study, 935 cases of T2DM patients were recruited in T2DM group, and 971 healthy examination individuals were selected as normal control.The TCF7L2 gene polymorphism was detected by matrix-assisted laser desorption/ionization time-of-flight mass spectrum.RESULTS: Significant differences in the frequencies of CC, CT and TT genotypes and the frequencies of C and T alleles on TCF7L2 rs7903146 were found between T2DM group and control group(P<0.05).As compared with C allele, the patients with T allele had a significantly higher risk of T2DM with OR of 1.190 (95% CI: 1.034~1.371).As compared with CC genotype, the patients with CT genotype had a significantly higher risk of T2DM with OR of 1.374 (95% CI: 1.122~1.683), and the patients with CT+TT genotype had a significantly higher risk of T2DM with OR of 1.307 (95% CI: 1.090~1.567).The levels of fasting plasma glucose, serum creatinine and blood urea nitrogen were higher in all participants with CT+TT genotype of rs7903146 than those with CC genotype, which showed a significant difference (P<0.05).CONCLUSION: The polymorphisms of rs7903146 in TCF7L2 gene may be associated with T2DM in Uygur population from Xinjiang region.The T allele and CT genotype of rs7903146 are the risk factors for T2DM.