OBJECTIVE To investigate the significance of expression of p53, Mdm2 and p21WAF/ CIP1 proteins and their relationships. METHODS Pathological specimens from thyroid carcinoma, adjacent non-tumor thyroid tissues and thyroid benign lesions were examined for p53, Mdm2 and p21WAF/CIP1 proteins by tissue microarray technique and immunohistochemistry method. RESULTS The positive expression rate of p53, Mdm2 and p21WAF/CIP1 in thyroid carcinoma was 50.6 %(37/73), 63.0 %(47/73) and 38.4 %(28/73) respectively. The expression of p53 and Mdm2 increased(P

Objective To investigate the clinical curative effect of α-lipoic acid combined with lipid microsphere alprostadil in treating elderly early diabetic kidney disease .Methods One hundred and sixty-four cases of early diabetic kidney disease treated in this hospital from January 2014 to January 2016 were selected and randomly divided into the experimental group and the control group according to the random number table method ,82 cases in each group .The control group was given lipid microsphere alpros-tadil ,while on this basis the experimental group was added with α-lipoic acid .The various biochemical indexes ,clinical efficacy and subcutaneous bruising ,fundus event occurrence were compared between the two groups .Results The levels of U-RBP and NAG after treatment in the two groups were significantly decreased compared with before treatment ,the difference was statistically sig-nificant (P<0 .05) .Although other indicators such as serum Scr ,BUN ,CysC andβ2-MG had different degrees of decline ,but the difference was not statistically significant (P>0 .05) .The effective rate in the experimental group was 91 .46% ,which was signifi-cantly higher than 78 .05% in the control group ,the difference was statistically significant (P<0 .05) .Subcutaneous bruising and fundus events in the experiment group had the increasing trend ,but the difference between the two groups had no statistical signifi-cance (P>0 .05) .Conclusion α-lipoic acid combined with lipid microsphere alprostadil in treating elderly early diabetic kidney dis-ease can better improve glomerular and renal tubular function .

Objective To investigate the correlation of circular RNA-ZNF532(cZNF532)expression level with pancreatic islet cell function and microvascular complications in patients with type 2 diabetes mellitus(T2DM).Methods A total of 198 T2DM patients admitted to the Department of Endocrinology,Nanyang Central Hospital from June 2018 to June 2022 were selected as the research subjects(observation group),and 50 healthy volunteers who underwent physical examination in the hospital during the same period were selected as the control group.The expression level of cZNF532 in peripheral blood mononuclear cells was detected by real-time fluorescence quantitative polymerase chain reaction,the fasting blood glucose(FPG),insulin(FINS),glycosylated hemoglobin(HbA1c),homeostatic model assessment of insulin resistance(HOMA-IR)and homeostasis model assessment of β-cell function(HOMA-β)of the subjects were detected by the fully automated biochemical analyzer,and the correlation between cZNF532 expression level and pancreatic islet cell function in T2DM patients was analyzed by Pearson correlation analysis.The clinical data of T2DM patients were collected,and they were divided into microvascular complication group(n=120)and non-microvascular complication group(n=78)according to whether microvascular complications occurred during treatment.Multivariate logistic regression analysis was used to identify the risk factors for microvascular complications in patients with T2DM,and Spearman correlation analysis was used to explore the correlation between the expression level of cZNF532 and microvascular complications in patients with T2DM.Results Compared with the control group,the levels of cZNF532,FINS,HbA1c and HOMA-IR were significantly higher and HOMA-β was significantly lower in the observation group(P＜0.05).The results of Pearson correlation analysis showed that the expression level of cZNF532 in T2DM patients was positively correlated with the FINS,HbA1c and HOMA-IR(r=0.324,0.357,0.410;P＜0.05);and negatively correlated with HOMA-β(r=-0.389,P＜0.05).There was no significant difference in gender,age,course of disease,number of patients with hypertension,high-density lipoprotein cholesterol(HDL-C),total cholesterol(TC)and triglyceride(TG)levels between the microvascular complication group and the non-microvascular complication group(P＞0.05);compared with the non-microvascular complication group,the expression levels of LDL-C,FPG and cZNF532 in the microvascular complication group were significantly higher(P＜0.05).Multivariate logistic regression analysis showed that LDL-C,FPG and cZNF532 were independent risk factors for microvascular complications in T2DM patients.The results of Spearman correlation analysis showed that the expression level of cZNF532 was positively correlated with the occurrence of microvascular complications in T2DM patients(r=0.681,P＜0.05).Conclusion cZNF532 is overexpressed in T2DM patients,which is associated with pancreatic islet cell function and microvascular complications in T2DM patients.