Objective To investigate the inhibition of Jin’s hemorrhoid ointment extract on proliflation of human umbilical vein endothelial cell (ECV-304), then to study the mechanism of treating hemorroid. Methods MTT assay was used to observe the inhibition of various concentrations Jin’s hemorrhoid ointment extract and single Chinese Herbs of compound on human umbilical vein endothelial cell (ECV-304). Results Various concentrations Jin’s hemorrhoid ointment extracts inhibited proliflation of human umbilical vein endothelial cell, which depended on concentration. All of Rheum decoctions and high concetration Curcuma decoction inhibited ECV-304 proliflation. Conclusion Jin’s hemorrhoid ointment can inhibit proliflation of human umbilical vein endothelial cell, and Rheum maybe the main effective part of Jin’s hemorrhoid ointment, Curcuma also take part in it.