Pathohistological observations on the soft tissue wounds experimentally produced with steel-bullets of three different weights and 8 different velocities were carried out on 42 dogs. It was found that if the weight of the bullets was identi- cal while their velocities were different, the severity of the injury and the extent of the contusion zone were directly related to the velocity of the bullet. The characteristic feature was that the injury at the entrance of the bullet was more severe than that at the exit. It might be resulted from the fact that the energy of the bullet was rapidly delivered soon after it penetrated the skin because of its light weight and the high resistance of the tissues.

Objective:To identify effective biomarkers for glioma patients.Methods:The mRNA expression profiles of 464 glioma patients with complete clinical follow-up information were downloaded from the Chinese Glioma Genome Atlas (CGGA). Weighted gene co-expression network analysis (WGCNA) was used to identify gene modules related to World Health Organization (WHO) grading of glioma, and univariate and multivatiate Cox regression analysis were performed to identify gliomas survival-related genes.Results:In weighted gene co-expression analysis, the module Brown was significantly positively correlated with glioma WHO stage ( r=0.55, P<0.05). In univariate analysis, five genes (TAGLN2, IGFBP2, METTL7B, ARAP3, PLAT) that were most significantly associated with clinical prognosis were selected for multivariate survival analysis, and the prognosis model was established to calculate the risk score. The receiver operating characteristic curve (ROC) confirmed that the risk score had high accuracy in predicting the 1-, 3-, 5-year survival rate of glioma patients. The above survival analysis results were verified in the Cancer Genome Atlas (TCGA) database. Conclusions:We use mRNA expression profiles to establish prognostic markers for gliomas to assess the overall survival of patients with glioma.

Objective: To investigate the expression changes of TNF ? mRNA after brain explosive injury in dogs. Methods: With the brain explosive injury model we had developed, nuclei acid in situ hybridization with an antisense RNA probe and computer image analysis were used to detect the dynamic changes of TNF ? mRNA in the cerebral cortex, hippocampus, hypothalamus, and up brain stem after brain injury. Results: The expression of TNF ? mRNA could be found in several regions of normal dog's brain tissues, and the expression became more obvious and widespread following explosive injury to the brain. In all of the brain regions, the expression changes of cerebral contussive area and hippocampus were more significant. TNF ? mRNA expression increased significantly at 30 min and reached maximum at 1 h after the explosive injury, and then decreased gradually, which was higher than normal's. More expression were detected in the ipsilateral hemisphere than contralateral hemisphere. Conclusion: The expression of TNF ? mRNA in basal condition implicate that TNF ? may play an important role in neural function. It is suggested that TNF ? may play an important role in traumatic brain injury. [

Objective:To evaluate the efficacy and safety of digital cholangioscopy-assisted non-radiation endoscopic retrograde cholangiopancreatography (ERCP) for common bile duct stones.Methods:Clinical data of patients who underwent digital cholangioscopy-assisted non-radiation ERCP for common bile duct stones from May 2019 to September 2021 were reviewed. The baseline data, the success rate of cannulation, the one-time success rate of endoscopic stone removal, the operation time, total hospital stay, complications, and recurrence of bile duct stones were analyzed.Results:A total of 170 patients were included, and bile duct stones were detected in 156 (91.8%) patients with the long diameter of 7.7±4.1 mm under preoperative imaging examination. Bile duct stones were detected under choledochoscopy and were successfully removed by using digital cholangioscopy through non-radiation ERCP. The success rate of cannulation was 100.0% (170/170) . The one-time success rate of endoscopic stone removal was 96.5% (164/170), and 6 patients (3.5%) received secondary stone removal for large stones (long diameter>30 mm).The time of biliary exploration and whole non-radiation ERCP were 9.6±2.7 min (6-24 min) and 35.9±17.3 min (13-85 min), respectively. The total hospital stay was 6.3±2.2 days (5-10 days). Postoperative pancreatitis occurred in 3 patients (1.8%), all of whom were mild and resolved after symptomatic treatment. No recurrence of bile duct stones was seen in any patient over 1-month postoperative follow-up.Conclusion:Digital cholangioscopy-assisted non-radiation ERCP is safe and effective for common bile duct stones without ray exposure, which is worth of promotion.

Objective:To evaluate a newly designed basket fit for digital cholangioscope in non-radiation endoscopic lithotomy for common bile duct stones.Methods:Seventy-eight patients who underwent non-radiation endoscopic removal of common bile duct stones at Zhongda Hospital Southeast University between February and October 2021 were enrolled in the randomized controlled trial, and were randomly assigned into the new basket group ( n=35) and the conventional basket group ( n=43) by drawing lots. Procedures of cholangioscope-based biliary exploration and stone removal by baskets, volume of water irrigation, time lengths of biliary exploration, stone removal and whole endoscopic procedure, and adverse events were compared. Results:Compared with the conventional basket group, the new basket group needed less biliary explorations (1.08±0.28 VS 2.30±0.51, t=-12.535, P<0.001), smaller volume of water irrigation (46.14±11.89 mL VS 78.62±10.09 mL, t=-13.052, P<0.001), and shorter time of biliary exploration and stone removal (9.69±2.97 min VS 12.67±2.51 min, t=-4.815, P<0.001) and whole endoscopic procedure (30.17±7.19 min VS 33.44±6.69 min, t=-2.076, P=0.041). The incidence of adverse events was not significantly different between the two groups [5.7% (2/35) VS 11.6% (5/43), χ2=0.826, P=0.363]. Conclusion:This newly designed basket can facilitate digital cholangioscope-guided non-radiation endoscopic removal of common bile duct stones with the advantages of shorter operation time, less exploration time of choledochoscopy and smaller volume of water irrigation.

This study was aimed to establish a suitable consistency evaluation system according to the existing problems in the quality evaluation of traditional Chinese medicine (TCM) for the development of Chinese medicine industry.Based on the particularity of TCM,combined with the advanced technology of TCM quality evaluation through the construction of TCM quality consistency evaluation system,this study explored a new quality evaluation method for seeking new breakthrough.The results showed that a new quality evaluation model of TCM using multiple indexes,which included "trait evaluation + chemical evaluation + biological evaluation" to achieve the consistency of TCM by effective combinations.It was concluded that the exploration provided some references for the evaluation of quality consistency of TCM.

Objective : To analyze and verify the factors influencing the prediction model of suicidal ideation of burn patients in hospital based on international scale． Methods : The clinical data of 194 burn patients treated in hospi- tal were retrospectively analyzed．General data questionnaire，ISI，HAMD，HAMA，ASDS and BSHS-B were used to evaluate the influencing factors of suicidal ideation．According to the presence or absence of suicidal ideation， the patients were divided into the suicidal ideation group and the non-suicidal ideation group．The baseline data be- tween the groups were compared，univariate screening of meaningful variables was conducted，and multivariate Lo- gistic regression modeling was further conducted. ROC analysis evaluated model differentiation，and internal verifi- cation was conducted. Results : According to the baseline data analysis results，there were no statistically signifi- cant differences in age，BMI，years of education，smoking history，estimated percentage of burned area，head and neck burns，hip and perineal burns，and pain scores in the suicidal ideation group (21 /194) compared with the non-suicidal ideation group ( 173 /194) ．Gender (P = 0. 047) ，presence or absence of trunk burn (P = 0. 022) ， severity of burn ( moderate burn : P = 0. 002 ; severe burn : P = 0. 458 ; extremely severe burn : P = 0. 169) ，ISI score (P = 0. 001) ，HAMD score (P = 0. 001) ，HAMA score (P＜0. 001) ，ASDS score (P = 0. 003) ，BSHS-B score (P = 0. 011) had statistical significance．Multivariate Logistic regression analysis showed that the severity of burn (moderate burn : OR = 0. 103，P = 0. 009 ; severe burn : OR = 0. 351，P = 0. 223 ; extremely severe burn : OR = 0. 103，P = 0. 095) and HAMA score ( OR = 1. 136，P = 0. 007) were independent influencing factors for burn patients with suicidal ideation 。The Logistic regression prediction model was established by two independent influ- encing factors. ROC analysis results showed that the model had good differentiation ( AUC = 0. 880，95% CI : 0. 808 －0. 952 ，P ＜0. 001 ) and the internal verification accuracy was 79. 38%． Conclusion The prediction model built on the basis of two independent influencing factors，burn severity and HAMA score，has a good predic- tion accuracy，which is helpful for clinicians to intervene as soon as possible for burn patients with suicidal ideation in hospital，in order to reduce the incidence and enrich clinical psychological research.