BACKGROUND:Studies have found that a variety of biological materials can be used for preparing corneal stroma scaffolds that have good biocompatibility, but research on preparation and biocompatibility of the acel ular porcine corneal stroma scaffold is little. OBJECTIVE:To explore the preparation and biocompatibility of the acel ular porcine corneal stroma scaffold. METHODS:Acel ular porcine corneal stroma scaffold and its extract were prepared. Wel-grown human corneal stromal cel s were selected and cultured in the extract of acel ular porcine corneal stroma scaffold (experimental group) or in the complete medium (control group), respectively. After 1, 2 and 3 days of culture, the proliferation ability of human corneal stromal cel s was detected by MTT assay. In the meanwhile, human corneal cel s were directly seeded onto the acel ular porcine corneal stroma scaffold, and then the cel growth on the scaffold was detected using immunochemical method. RESULTS AND CONCLUSION:The number of human corneal stromal cel s was in a rise with time in the two groups, and absorbance values had no significant difference between two groups at different time points of culture. Human corneal stromal cel s grew wel on the scaffold, and were positive for cel integrinβ1, vimentin, aldehyde dehydrogenase 3A1, as wel as CD34, CDK2 and K-Ras. These results show that the acel ular porcine corneal stroma scaffold has no cytotoxicity, and has good biocompatibility.

Objective:To explore effects of perioperative application of NNN-linked nursing model on self-perceived burden, postoperative rehabilitation and quality of life in patients undergoing hip-knee replacement.Methods:A total of 100 patients with osteoarthritis who were scheduled to undergo artificial hip-knee replacement in the First People's Hospital of Lianyungang from July 2018 to July 2019 were selected by stratified sampling method and they were randomly divided into the control group and the observation group according to the sealed envelope method, with 50 patients in each group. The control group was given routine perioperative nursing while the observation group was given perioperative nursing based on NNN-linked nursing model. Self-Perceived Burden Scale (SPBS) was used to evaluate self-perceived burden before surgery and at 3 days after surgery, American Knee Society knee score (AKS) was used to measure the active and passive activity of knee joints and the knee visual analogue score (VAS) was used to evaluate the knee rehabilitation in the two groups at 1 day and 2 weeks after surgery. Nursing Outcomes Classification-Patient Satisfaction Survey Scale was used to investigate nursing satisfaction of patients before discharge in two groups. MOS 36-Item Short Form Health Survey (SF-36) was used to assess the quality of life in the two group before surgery and at 3 days after surgery.Results:At 3 days after surgery, SPBS scores in the two groups were significantly decreased compared with those before surgery, and the score in the observation group were significantly lower than that in control group ( P<0.05) . AKS score at 1 day and 2 weeks after surgery in observation group were respectively (27.26±6.81) and (103.26±15.22) , which were significantly higher than (24.28±5.68) and (95.32±10.83) in the control group ( P<0.05) . The active range of knee joint motion and passive range of knee joint motion at 1 day and 2 weeks after surgery in the observation group were respectively (47.66±9.22) °, (81.23±14.22) ° and (64.23±12.22) °, (89.23±14.26) °, which were significantly greater than (42.25±8.59) °, (72.26±12.52) ° and (58.26±10.12) °, (78.26±13.10) ° in the control group ( P<0.05) . VAS scores at 1 day and 2 weeks after surgery in the observation group were respectively (5.29±0.82) and (3.46±0.77) , which were significantly lower than (5.91±0.88) and (4.22±0.98) in the control group ( P<0.05) . The improvements in AKS score, active and passive knee motion range and VAS score at 2 weeks after operation in both groups were greater than 1 day after operation ( P<0.05) . The nursing satisfaction scores of communication, safety, nursing technology, nursing, guidance and physical environment and total score of satisfaction in observation group were significantly higher than those in the control group ( P<0.05) . At 3 days after surgery, scores of physical functioning, physical role, body pain, general health, vitality, social function, emotional function and mental health of SF-36 in the two groups were significantly increased compared with those before surgery ( P<0.05) , and the scores in observation group were higher than those in the control group ( P<0.05) . Conclusions:Application of NNN-linked nursing model in perioperative nursing of patients with hip-knee replacement can reduce the self-perceived burden, improve knee function and active and passive range of motion, relieve knee pain and improve nursing satisfaction and quality of life.