Objective:To investigate the effect of ets 1 antisense oligoxydeonucleotide(ASODN)on invasive ability of gastric cancer cell line SGC 7901 in vitroMethods:Cultured cells were divided into three groups:control group,sense oligoxydeonucleotide(SODN)group and ASODN group.After transfection for 24h,cultured cells were harvested to detect the changes of ets lmRNA by the reverse transcription polymerase chain reaction method(RT PCR),growth inhibition was detected by colony forming unit assay,invasive ability was measured by the number of cells to penetrate polycarbonates coated with or without matrigel.Results:est 1 ASODN could underregulate the expression of ets 1mRNA,number of colony forming was significantly lower than the other two groups(24.2?4.8,47 6?8 1,44 3?7 6,P

Objective: To investigate the therapeutic effects of ets-1 antisense oligoxydeonucleotide(AsODN) on gastric carcinoma. Methods: Cultured SGC-7901 cells were devided into control group, sense oligoxydeonucleotide(sODN) group and AsODN group. After transfection for 24 h, expression of ets-1mRNA was detected by RT-PCR, growth inhibition was detected by colone formation assay, in vitro invasive ability assay was carried out in Transwell chamber,the animal model of xenotransplanted gastric carcinoma in nude mice was established to detect invasive ability in vivo. Results: ets-1 AsODN could under-regulate the expression of ets-1 mRNA, number of colone formation of AsODN group was significantly lower than the other two groups(24.2?4.8 vs 47.6?8.1 vs 44.3?7.6, P

Objective To explore the impact of severe weather on road traffic injuries (RTIs). Methods Relevant literature on the impact of meteorology on the occurrence and resulting casualties of road traffic injuries was searched. Meta-analysis was performed on the included literature using state16.0 software. Results A total of 28 articles were included. The results of meta-analysis showed that heat wave, cloudy day, snowy day, rainy day and other severe weather such as storm had a statistically significant impact on the occurrence of road traffic injuries. Severe weather such as storms had a statistically significant impact on RTIs casualties. Conclusion Heat wave, cloudy day, snowy day, rainy day, storm and other bad weather are independent risk factors for the occurrence of RTIs. Storm is a risk factor for accident casualties. There is not enough evidence to show that low temperature, cold wave and heavy fog are the influencing factors of road traffic accidents.