Objective To compare the efficiencies of enteral nutrition with nasogastric tube with that of enteral nutrition with endoscopic gastrostomy tube in ICU long-term bedridden patients. Methods A total of 6 patients who were admitted in our ICU between January 2008 and September 2009 were enrolled in this study. The expected completion of daily calorie supply, completion of total volume of nutrient fluid feeding, nutrient fluid backstreaming condition, and incidence rate of tube obstruction were compared between those supported with nasogastric tube and endoscopic gastrostomy tube. Results The completion of daily calorie supply and completion of total volume of nutrient fluid feeding were significantly superior in patients who were supported with endoscopic gastrostomy tube than those with nasogastric tube (P = 0. 002 and P = 0. 008, respectively). In addition, nutrient fluid backstreaming condition and incidence rate of tube obstruction were significantly lower than with nasogastric tube (P= 0. 011 and P = 0. 021, respectively). Conclusion Enteral nutrition with endoscopic gastrostomy tube is more efficient in reaching the nutrition support target than with nasogastric tube.

Objective To investigate fluid management and risk factors of intra-abdominal hypertension (IAH) after postpartum hemorrhage.Methods Clinical data of 64 patients of postpartum hemorrhage who were admitted to Intensive Care Unit (ICU) of the First People's Hospital of Kunming from January 2013 to January 2015 were collected.The patients were divided into IAH group and non-IAH group based on intra-abdominal pressure on admission to ICU.Diuresis or dialysis were offered to patients whose output exceeded 1 000 ml of their input after hospitalization.The background information,including maternal age,existence of pre-eclampsia and whether hysterectomy and transcatheter arterial embolization were performed,intra-abdominal pressure,liver and renal function and length of stay in hospital between the two groups were compared.The intra-abdominal pressure and liver and renal function before and 24 h after negative fluid administration of all subjects were compared as well.Independent-samples t test,paired-samples t test,Chisquare test and logistic regression analysis were applied for statistics.Results Among all of the 64 patients,15(23,4％) presented with IAH on admission,25 (39.1％) were complicated with preeclampsia,one (1.6％) had the uterus removed,and 16 (25.0％) had transcatheter arterial embolization performed.Within 12 h before admission,the average blood loss of the 64 women was (4 022± 1 275) ml,crystal solution input was (8 894±2 597) ml,colloidal fluid input was (343 ± 87) ml,blood products input was (1 370± 346) ml,total fluid input was (10 607± 2 825) ml,total fluid output was (5 176±2 334) ml,average fluid input per hour was (884±235) ml and average urinary production per hour was (431 ±195) ml.Logistic regression analysis showed that pre-eclampsia (OR=5.30,95％CI:1.15-24.45),average fluid input per hour ＞ 1 000 ml (OR=5.34,95％CI:1.14-24.92) and average urinary production per hour ≤ 200 ml (＞200 ml,OR=0.17,95％CI:0.05-0.58) were risk factors of IAH.The non-IAH group showed shorter length of stay in ICU [(3.33± 1.84) vs (8.73 ±9.77) d] and shorter length of stay in hospital [(10.29±3.96) vs (18.13±9.88) d] than IAH group (t=-3.71 and-4.55,both P＜0.05).After 24 hours negative fluid administration,the intra-abdominal pressure [(6.67 ± 4.61) vs (8.47 ± 5.85) mmHg (1 mmHg=0.133 kPa),t=7.76],total serum bilirubin level [(14.31±14.91) vs (20.96 ± 37.56) μ mool/L,t=2.02],blood urea nitrogen level [(6.49±5.18) vs (7.57±7.07) mmol/L,t=2.72] and creatinine level [(105.57±81.66) vs (140.61 ± 126.14) μmol/L,t=5.33] were all significantly decreased comparing with before negative fluid administration,but the serum albumin level rised up [(24.45 ± 4.80) vs (21.35 ±5.69) g/L,t=-4.47].Conclusions Pre-eclampsia,massive fluids input and too little output per hour in patients complicated with postpartum hemorrhage were risk factors of IAH.IAH is harmful to liver and kidney,and makes the length of stay in hospital longer.However,negative fluid administration could decrease the intraabdominal pressure and improve the function of liver and kidney.

Objective To study the correlation between fluid management during liver transplantation and postoperative intra-abdorninal hypertension (IAH),and the correlation between intra-abdominal pressure and hemodynamics during liver transplantation.Method From Sep.2008 to Sep.2014,95 cases were admitted to ICU following liver transplantation were enrolled.All recipients were given abdominal color ultrasound examinations,and the IAH was measured.The preoperative Child-Pugh score,total operating time,anhepatic phase time,intraoperative blood loss,crystal solution input,colloidal fluid input,red blood cell infusion,plasma infusion,intraoperative total fluid input,intraoperative fluid input per h,and urinary volume per h and their correlation with postoperative IAH were analyzed by the Logistic regression method.The relationship between hemodynamics data and IAH intra-abdominal pressure was analyzed by the correlation analysis of two variables.Result IAH occurred in 18.94％ (18/95) recipients at 72nd h after operation.Fluid input per h (＞2000 ml/h) was risk factor (B=1.62;P＜0.05;OR=5.07,95％ CI:1.41-18.23) of IAH.Urinary volume per h (＜200 ml/h) is risk factor (B=-3.21 ;P＜0.01:OR =0.04,95％ CI:0.01-0.18) of IAH.There was correlation between hepatic artery peak flow velocity (r =0.83,P＜0.01),portal vein peak flow velocity (r =-0.182,P＜0.05),and retrohepatic inferior vena cava peak flow velocity (r=-0.184,P＜0.05) with IAH.Conclusion Fluid input per h should be controlled in a low level and urinary volume per h should be increased in order to prevent IAH.There is correlation between hepatic artery peak flow velocity,portal vein peak flow velocity and retrohepatic inferior vena cava peak flow velocity with IAH.

Objective To assess the value of early evaluation of left ventricular global systolic function in patients with metabolic syndrome(MS) by three-dimensional speckle tracking echocardiography (3D-STI).Methods 33 healthy subjects and 41 MS subjects who didn't have the left ventricular remodeling were recruited in this study,and the myocardial motions were tracking by 3D-STI.The parameters of left ventricular global longitudinal peak systolic strain(LS),circular peak systolic strain(CS),radial peak systolic strain(RS) and area peak systolic strain (AS) and the values of body mass index (BMI),waist circumference,waist-to-hip ratio and biochemical indicators were compared between the two groups.Results Compared with controls,LS of MS group was significantly decreased (P ＜ 0.01),while no significantly differences were found in CS,RS,and AS(P ＞0.05).The BMI,waist circumference and waist-to-hip ratio were positive correlated with LS (r =0.559,0.617,0.681,P ＜0.01).Conclusions 3D-STI could early evaluate the changes of left ventricular global systolic function for metabolic syndrome patients,and longitudinal peak systolic strain could be the most sensitive index.

Objective:To compare hemodynamic changes under general anesthesia with those under general + epidural anesthesia during endotracheal intubation in senile patients.Methods:Forty ASA Ⅰ or Ⅱ senile patients aged 65-75 years were equally randomized into a G(general anesthesia) and a GE(general + epidural anesthesia) group and received intravenous injection of sufentanil 0.2 ?g/kg,midazolam 0.06 mg/kg,vecuronium 0.12 mg/kg and propofol 1.6 mg/kg for general anesthesia induction and endotracheal intubation.SBP,DBP,HR,EDV,SV,EF and CO were recorded at 5 different time points,i.e.,before induction(T0),just before intubation(T1),immediately after intubation(T2),1 minute after intubation(T3),and 5 minutes after intubation(T4) via ultrasoundcardiogram.Results:Significant hemodynamic changes were observed in both groups(P

Objective To explore the function of p53 on regulating the expression of miR-148b in lung cancer cell line PC-9 and its corresponding molecular mechanism and the impact on cell proliferation. Methods Transient transfection of p53 eukaryotic expressing plasmids into lung cancer cell line PC-9 was performed to establish a cell model over-expressing p53. RT-PCR was used to explicit the impact of p53 on the expression of miR-148b. A reporter vector containing miR-148b promoter was used to investigate the function of p53 on regulating the transcription of miR-148b. Low-expressing miR-148b by transfecting its specific inhibitors , a CCK-8 assay was performed to explore the influence of miR-148b on the lung cancer cell proliferation inhibited by p53. Results Over-expression of p53 promoted miR-148b expression in lung cancer cell line PC-9. P53 could increase the luciferase activity driven by miR-148b promoters. Knockdown of miR-148b attenuated the impact of p53 on inhibiting the proliferation of PC-9 cells. Conclusion P53 inhibits the proliferation of lung cancer cell line PC-9 partially depending on miR-148b.

Objective To investigate early postoperative impact on recipients after liver transplantation with the two hemodynamic management strategies by using the "gold standard" Swan-Ganz catheter and central venous catheter (CVC) to monitor hemodynamics and guide liquid and vasoactive drug therapy.Methods From May 2006 to January 2009,102 from 107 cases of liver transplantations in our hospital were randomly divided into two groups:PAC group (pulmonary artery Swan-Ganz catheter group,n = 52) and CVC group (n = 50 cases).Hemodynamic parameters were monitored.Results There was no significant difference in the general characteristics between two groups In PAC group and CVC group,the mortality during ICU stay was 7.7 % and 4 %; postoperative 28-day mortality was 11.5 % and 8 % ; the median mechanical ventilation time was 26.5 and 24 h; the median ICU stay was 2.5 and 2 days,respectively.There was no significant difference in the aforementioned parameters (P＞0.05).There was no significant difference in renal function and liver function before and 1,5 days after operation,and mean blood lactic acid level 1,2,and 3 days after operation between two groups (P＞0.05).In PAC group and CVC group,transient ventricular arrhythmias occurred in 26 cases and 2 cases Conclusion It is not a good strategy using "gold standard" Swan-ganz catheter to monitor hemynamic parameters and guide therapy after liver transplantation in ICU,and on the contrary,the application of "gold standard" Swan-ganz catheter increases incidence of transient ventricular arrhythmias.

Objective:To compare the efficacy and safety of stone extraction with a single peroral choledochoscopy system under direct visualization and conventional X-ray endoscopic retrograde cholangiopancreatography (ERCP) for non-difficult common bile duct stones.Methods:A total of 164 patients with common bile duct stones who underwent stone extraction by using the single peroral choledochoscopy system under direct visualization (the observation group, n=82) and conventional X-ray endoscopic retrograde cholangiopancreatography (ERCP) (the control group, n=82) from January 2018 to April 2022 in Dongfang Hospital, Beijing University of Chinese Medicine were enrolled. The observation group was directly selected from the database, while the control group was randomly matched by age stratification with baseline data validated. The success rates of intubation, stone removal, postoperative complication incidence, and radiation exposure between the two groups were compared. Results:There was no significant difference between the baseline data of the observation group and the control group ( P>0.05). The number of patients with detected stones≥2 in the observation group and the control group were 59 (71.95%) and 37 (45.12%) respectively with significant difference ( χ 2=12.16, P=0.001). The success rates of bile duct intubation in the observation group and the control group were both 100.00% (82/82). The success rates of stone extraction were 98.78% (81/82) and 100.00% (82/82) respectively with no significant difference ( P>0.05). The one-time stone removal rates of the two groups were 93.90% (77/82) and 92.68% (76/82) respectively with no significant difference ( χ2=0.10, P=0.755). There was no significant difference in the incidence of postoperative complications between the observation group and the control group ( P>0.05). The amount of intraoperative ray exposure volume in the observation group was significantly lower than that of the control group [10.20 (6.69, 18.94) mGy VS 15.41 (10.70, 22.77) mGy, U=2 462.00, P=0.003]. Conclusion:The efficacy and safety of stone extraction with single peroral choledochoscopy system under direct visualization are comparable to those of traditional X-ray ERCP for non-difficult common bile duct stones, but it can significantly reduce the intraoperative ray exposure volume during therapeutic ERCP stone extraction.

Objective To explore the effects of bronchoalveolar lavage combined with microbiological rapid on-site evaluation in potential donor lung maintenance.Methods Brain death patients who met the inclusion criteria and were admitted to the Intensive Care Unit(ICU)of Calmette Hospital Affiliated to Kunming Medical University from September 2020 to December 2022 were selected for bronchoalveolar lavage(BAL)and(BAL)and the lavage fluid were collected for M-ROSE to compare the pathogen detection rate and initial diagnosis time.According to the positive results of the microbiological rapid on-site evaluation,patients with the brain death were treated with empirical anti-infective therapy,and the oxygenation index,chest X-ray score,and the infection index(WBC,CRP,PCT)of anti-infective treatment 48 hours were evaluated.Results 1.Comparison of the detection rate of pathogenic microorganisms:The results of M-ROSE were highly consistent with a routine microbiological smear(Kappa = 0.921,P<0.001).2.Comparison of diagnostic time:The initial diagnosis time of M-ROSE was significantly lower than routine microbiological smear time and microbial culture time(P<0.001).3.Comparison of therapeutic effects of anti-infective therapy for 48 hours:There was no significant difference in oxygenation index,white blood cells and hypersensitive C-reactive protein before and after the anti-infective treatment(P>0.05).There were significant differences in procalcitonin and chest X-ray before and after the anti-infective treatment(P<0.05).Conclusion Bronchoalveolar lavage combined with microbiological rapid on-site evaluation has the high timeliness in the diagnosis of potential donor pulmonary infection,which can provide a preliminary basis for the early anti-infective therapy of donor lung maintenance.

Phosphatidylinositol 3-kinase (PI3K) signaling plays an important role in the regulation of cellular lipid metabolism and non-alcoholic fatty liver disease (NAFLD). However, little is known about the role of the regulatory subunits of PI3K in lipid metabolism and NAFLD. In this study, we characterized the functional role of PIK3R3 in fasting-induced hepatic lipid metabolism. In this study, we showed that the overexpression of PIK3R3 promoted hepatic fatty acid oxidation via PIK3R3-induced expression of PPARα, thus improving the fatty liver phenotype in high-fat diet (HFD)-induced mice. By contrast, hepatic PIK3R3 knockout in normal mice led to increased hepatic TG levels. Our study also showed that PIK3R3-induced expression of PPARα was dependent on HNF4α. The novel PIK3R3-HNF4α-PPARα signaling axis plays a significant role in hepatic lipid metabolism. As the activation of PIK3R3 decreased hepatosteatosis, PIK3R3 can be considered a promising novel target for developing NAFLD and metabolic syndrome therapies.
Animals ; Diet, High-Fat ; Fatty Liver ; Lipid Metabolism ; Mice ; Non-alcoholic Fatty Liver Disease ; Phenotype ; Phosphatidylinositol 3-Kinase