Objective:To establish a multidrug resistant human salivary adenoid cystic carcinoma cell line. Methods:Salivary adenoid cystic cells of the cell line SACC were exposed to 1 ?g/ml of DDP for 48 h at the interval of one month. 6 months later, the cell line was established and named SACC/DDP. MTT method was used to study the drug resistance of SACC and SACC/DDP cells against 4 chemotherapeutic agents(MTX, PYM, VCR, MMC). Flow cytometry was adopted to study the cell cycle distribution of the cells after treatment with 4 agents at different concentrations for 72 h. Results:The drug resistance of SACC/DDP cells against VCR,MTX,PYM and MMC was 2.9,2.4,1.1 and 1.8 times of that of SACC cells. The ratio of G0 cell number to G1 cell number in SACC/DDP cell line was smaller than that in SACC cell line after treatment with the 4 agents at different concentrations respectively.Conclusion:The multidrug resistant human salivary adenoid cystic carcinoma cell line can be estabished by exposing parent cell line SACC periodically to DDP.

Objective:To investigate the effects of rocuronium in the intravenous anesthesia in children with cleft lip and palate repair.Methods:According to random number table,96 children with cleft lip and palate aged 1-3 years underwent general anesthesia for surgery were divided into 3 groups (n =32).The children in group A,B and C received intravenous injection of rocuronium at 0.6,0.9 and 1.2 mg/kg,respectively.The anesthesia effects were observed.Accelerometry (TOF-Watch(@) SX) was used to monitor neuromuscular transmission.Results:Intubating conditions ranged from good to excellent in all children.The onset time of anesthesia in Group C was significantly shorter(P ＜ 0.05),the duration of neuromuscular block was significantly longer in group C than in group A and B (P ＜ 0.05).The recovery index in group C was significantly longer than that in group A and B (P ＜ 0.05,A vs B,P ＞ 0.05).Muscle relaxant residual incidence in group C was higher than that in group A and B (P ＜ 0.05,A vs B,P ＞ 0.05).Conclusion:Rocuronium at 0.6 mg/kg or 0.9 mg/kg are effective for excellent tracheal intubation,with faster onset time and recovery time,low incidence of postoperative residual curarization(PORC) in children with cleft lip and palate repair.

Objective By prokaryotic expression and purifying the human bocavirus recombinant protein VP2, to establish the indirect enzyme-linked immunoassay for detection of virus. Methods We amplified the human bocavirus recombinant protein VP2 gene fragments from WHL-1 template by PCR , and cloned into the expression vector pET28a, then conversed into the BL21 (DE3) and expressed the fusion protein detected by Western Blot detection , the obtained the antibody and detected the human bocavirus in serum in Guanghzhou area in healthy people. Results The Recombinant prokaryotic expression identified correct by double enzyme, and it could occur specific reaction with the virus positive serum. The best optimal antigen coating concentration were serum multiples and blocking BSA was 2 mg/mL , 1 ∶ 200 and 1%. The best working dilution of enzyme-labeled secondary antibody was 1 ∶ 4 000. The best working hours was 1h. This detection method had good specificity and reproducibility. The cut-off of the indirect ELISA method was 0.1 and the sensitivity and specificity of the developed ELISA method were 92% and 98% respectively. The coincidence rate of determination results by the developed kit and control kit was 97%. Conclusion The competitive ELISA established by prokaryotic expressing and purifying the human bocavirus protein VP2 protein , provides a basis in detecting the human bocavirus serum antibody.

Objective To analyze the antigens of solid cultured germ, liquid cultured germ and secreted composition, looking for the main immunity antigens of Mycobacterium tuberculosis H37Rv.MethodsShowing the difference of the antigens of solid cultured germ, liquid cultured germ and secreted composition by SDS-PAGE, Western blotting, monoclonal and polyclonal antibody techniques.Results Antigens of the solid cultured germ were nearly accordance with the liquid cultured germ and they were difference with secreted proteins. The main immunity antigens of the cultured germ were 79 000, 54 000 ,38 000~50 000, 28 000 and the secreted proteins are 66 000, 55 000~64 000, 30 000~34 000, 24 000, 16 000.The antigens of 66 000, 55 000 and 16 000 were proved that they were secreted proteins by monoclonal antibodies.Conclusions This study demonstrated that the main immunity antigens of the cultured germ in Mycobacterium tuberculosis H37Rv were difference with the secreted one .This is beneficial to the cognition of H37Rv and looking for special antigens for diagnosis of tuberculosis .

Objective To discuss the clinical value of the expression level of acute lower respiratory tract Boka virus (HBoV)in‐fectecd children whose detection serum specific antibody .Methods 904 cases of children with acute lower respiratory tract Boka vi‐rus infection hospitalized from March 2011 to July 2014 who were selected as study objects ,serum ,sputum ,bronchoalveolar lavage fluid HBoV DNA positive were as the gold standard for diagnosis of acute lower respiratory tract infection in HBoV ,the positive serum HBoV antibody of HBoV in children with acute lower respiratory tract infection was defined as the observation group ,serum HBoV antibody negative acute lower respiratory tract infection in children with HBoV was defined as the control group ,the correla‐tion between serum HBoV antibody and acute lower respiratory tract HBoV infection children whose clinical characteristics were analyzed .Results Serum HBoV antibody in the diagnosis of acute lower respiratory tract infection of HBoV whose sensitivity ,spe‐cificity ,positive predictive value ,and negative predictive value ,accuracy of diagnosis were separately 60 .32% 、90 .25% 、31 .67% 、96 .81% 、88 .16% .In the general data ,between the observation group and the control group in gender ,age ,hospitalization time , there were no significant differences(P>0 .05) .In the clinical manifestations ,nasal congestion and runny nose ,cough ,fever ,vomi‐ting and diarrhea ,shortness of breath ,breathing difficulties whose occur rates had no significant differences between the observation group and the control group(P>0 .05) ,the incidence of wheezing of the observation group was significantly higher than that of the control group ,the difference had statistical significance (P<0 .05) .The comparison of clinical diagnosis between the observation group and the control group had no significant difference(P>0 .05) .Conclusion Serum HBoV antibody is in favor of acute lower respiratory tract infection of HBoV in the diagnosis of exclusion ,and the serum HBoV antibody positive and acute lower respiratory tract infection of HBoV have a certain relationship in children with wheezing symptoms .

Objective To discuss the correlation between the level of serum cystatin C and lipids in patients with system lupus erythematosus.Methods Used automatic biochemical analyzer to detect serum cystatin C (CysC),triglyceride (TG),total cholesterol (TC),high density lipoprotein cholesterol (HDL-C),low density lipoprotein cholesterol (LDL-C)and hsCRP levels in 136 cases of SLE patients and 113 cases of healthy people.Data obtained using SPSS13.0 software to carry on sta-tistical analysis.Results Outcome of SLE patients group compared with healthy controls,hsCRP (13.5 ± 4.85 mg/L vs 2.03±0.88 mg/L),CysC (2.63±1.95 mg/L vs 0.85±0.37 mg/L),LDL-C (3.06±1.21 mmol/L vs 2.33±0.41 mmol/L),TC (5.32±2.63 mmol/L vs 4.02±1.67 mmol/L)and TG (1.92±0.83 mmol/L vs 1.44±0.8 mmol/L)were signifi-cantly higher the difference between groups was statistically significant(t=2.45~12.4,P <0.05).Compared with healthy controls,HDL-C (1.12±0.31 mmol/L vs 1.52±0.85 mmol/L)was decreased (P <0.01).In SLE patients group,the ser-um CysC level and hsCRP,TC,TG and LDL-C were positively correlated,and the level of HDL-C was negative to the level of CysC.The health control group was no significant correlation.Conclusion Serum lipid levels of SLE patients were posi-tive to the level of CysC.Suggest that joint detection of SLE patients serum CysC and blood lipids index is helpful to the di-agnosis of SLE treatment and condition monitoring.

Objective To investigate the changes of the expression of osteoprotegerin (OPG) and receptor activator nuclear factor kappa B ligand (RANKL) during the healing of mandibular fracture after the transection of the inferior alveolar nerve (IAN) in rabbits. Methods Forty rabbits were randomly divided into 2 groups and each group consisted of 20 animals. The rabbits of the first group underwent mandibular fracture and transection of IAN and those of the second group underwent mandibular fracture only. The rabbits were killed at 7, 14, 21, 28 day after fracture. The callus tissue of the rabbits was collected and paraffin sections of the callus tissue were prepared. The callus sections were subjected to HE staining. The effects of calcitonin gene-related peptide (CGRP) on the expression of OPG and RANKL were observed with immunohistochemical staining. Results There were only a few CGRP immunoreactive nerve fibers at day 7 after IAN section. Low level of OPG expression was found throughout the repair process but RANKL was intensively expressed in the rabbits of the first group. Conclusion CGRP can up-regulate the expression ratio of OPG/RANKL and promote the healing process of fracture. The loss of CGRP is unfavorable to bony healing.

Objective To evaluate the effects of recombinant human bone morphogenetic-2-polylactide sustained release nanospheres (rhBMP-2-PLA-Ns) on cultured rabbit osteoblasts in vitro. Methods Rabbit osteoblasts were cultured in vitro, and rhBMP-2-PLA-Ns was added into the medium of the third generation of rabbit osteoblasts. The expression of the proliferating cell nuclear antigen (PCNA) was examined by immunofluoreacence staining, and the formation of tuberculums observed with alizarin red staining. Western blot was used to detect the effects of rhBMP-2-PLA-Ns on the expression of vascular endothelial growth factor (VEGF), which was compared with that in rhBMP-2 group and blank group. Results There was no significant difference in the number of osteoblasts with positive PCNA expression among three groups five days later. Ten days later, the number of osteoblasts with positive PCNA expression in rhBMP-2-PLA-Ns group was significantly higher than that in rhBMP-2 group and blank group, which indicated that rhBMP-2-PLA-Ns could enhance the expression of PCNA. Compared with rhBMP-2 group and blank group, rhBMP-2-PLA-Ns could significantly enhance the formation of tuberculums, with statistical difference (P<0.05). The expression of VEGF was detected in all three groups, and the level of the VEGF expression in rhBMP-2-PLA-Ns group was significantly higher than that in the other 2 groups (P<0.05). Conclusion The biological activity of rhBMP-2-PLA-Ns is superior than that of rhBMP2, and rhBMP-2-PLA-Ns can promote the proliferation, mineralization of osteoblasts and the secretion of VEGF, which has a better prospect in facilitating traumatic bone healing.

Objective To establish a three-dimensional (3D) finite element model of a swine mandible, simulate the dynamic procedure of bullet damaging the swine mandible and explore a finite ele-ment analysis method on maxiilofacial gunshot wound. Methods The digital imaging and communica-tions in medicine (DICOM) data obtained from CT scanning of a swine mandible were remerged into a 3D finite element model of the original specimen through Mimics and ANSA software, then a simulation of 3D finite element model penetrated by a 7.62 mm bullet was carried out through LS-DYNA software. The simulation data were compared with those from animal experiment in laboratory to test the feasibility of 3D finite element model and the simulation method. Results A 3D finite element model of a swine mandi-ble was established, with highly identical geometric size with the specimen. In the meantime, the dynam-ic process of a 7.62 mm bullet damage to the model was successfully simulated. Data from the simulation and those from animal experiment showed a high level of consistency. Conclusion 3D finite element method is prosperous in application in basic research on maxillofacial gunshot wound.

Objective To investigate clinical value of the anti-nuclear antibody (ANA ) ,anti-double strand DNA (dsDNA ) anti-body and anti-extractable nuclear antigen(ENA) antibody repertoire in systemic lupus erythematosus (SLE) diagnosis .Methods 158 SLE patients were served as SLE group and another 50 healthy people as the control group .Indirect immunofluorescence(IIF) and immunoblotting test(IBT ) were employed to detect ANA ,anti-dsDNA antibody and anti-ENA antibody repertoire .Results Positive rates of ANA ,anti-dsDNA antibody and anti-ENA antibody repertoire in SLE diagnosis were 81 .65% ,68 .35% and 87 .34% ,respectively ,which were all lower than that of their combined detection (93 .04% ) ,with statistically significant difference (P<0 .05 ) .Among anti-ENA antibody repertoire ,the positive rate of anti-U1-nuclear ribonucleoprotein (U1-Nrnp ) antibody (52 .53% ) was the highest .Conclusion Combined detection of ANA ,anti-dsDNA antibody and anti-ENA antibodies repertoire has some clinical value of early diagnosis of SLE .