Objective To obtain information on viral molecular structure and genome via full-length genomic analysis on the norovirus strain GZ20133135 isolated from Guangzhou.Methods Primers were designed according to the Sydney2012 full sequence.The full genome of the strain GZ20133135 was amplified by RT-PCR.The whole genome sequence was analyzed after cloned and sequenced.Results The genome of G Ⅱ-4 norovirus strain GZ20133135 consisted of 7566 bp,and it was revealed that there were three ORFs composites ORF1 (5100 bp),ORF2 (1623 bp),ORF3 (807 bp) respectively; ORF1 and ORF2 had 19 nt overlap.It was found by evolutionary comparative analysis that GZ20133135 genomic nucleotide sequences,compared with reference strains of G Ⅱ-4 Sydney2012 strains,the highest homology with a total length of homology was 99.07％.Phylogenetic analyses showed GZ20133135 belonged to G Ⅱ-4Sydney 2012 variant.Data of 541 amino acid analyses showed that Sydney 2012 variant strains of popular sites were aa294V or A or P→T,aa296S or T→S,aa297H or Q→R,aa298D or N or T→N,aa368T or N or S or A→E,aa372 N or S→D,aa393 N or D or S,aa394 T or G or S→T,aa395T or A→T,aa407 N or D→S,aa412T or D→N,aa413G or I→T.Conclusion Norovirus GZ20133135 belonged to the G Ⅱ-4 Sydney2012 variant.In This study,GZ20133135 full sequence information can be used not only as a full-length NoV variant sequence standard for future comparison studies,but also as useful material for the public health by enabling the diagnosis,vaccine development,and prediction of new emerging variants.

After the first infection with varicella-zoster virus causes chickenpox and recovers, it will lurk in the spinal ganglia and other parts of the body. As people age and immunity declines, the virus reactivates and causes neurotropic bands of blisters that mainly affect the skin of the waist and chest, known as herpes zoster. At present, there is no specific drug that can prevent and control the infection and recurrence of varicella-zoster virus infection. In view of the unique immunogenic advantages of varicella-zoster virus glycoprotein E (gE), it has become an important target antigen for the development of herpes zoster vaccine at home and abroad. This article reviews the current research progress of new gE-based herpes zoster vaccine in the world, hoping to provide reference for the research and development of a new generation of herpes zoster vaccine in China.