Objective The study was designed to investigate the intestinal mucosa barrier function changes in rats with severe acute pancreatitis (SAP),and the intervention effect of epigallocatechin-3-gallate(EGCG) injection and its mechanisms of protection on intestinal mucosa barrier function injury of SAP with the purpose of providing evidence for preventing and curing intestinal mucosa barrier function injury of SAP in clinical practice.Methods A total of 80 healthy male Sprauge-Dawley (SD) rats were randomly divided into a normal group (Normal),a sham-operation group (Sham),a SAP model group (Model),and a EGCG treated group (EGCG),with 24 rats (except 8 rats in the normal group) in each group.The latter three groups each had three subgroups at three time points:6,12,24hours.Rats with SAP were prepared with retrograde cholangiopancreatic duct infusion in 5％ sodium taurocholate.The dynamic changes of the indexes of intestinal mucosa barrier function injury and Pancreas injury were observed at various time points in each group,including the levels of Plasma diamine oxidase (DAO) and serum amylase and malondialdehyde (MDA),nitric oxide(NO),inducible nitric oxide synthase(iNOS)in gut.Results 1.The levels of serum amylase [(3158.64 ± 218.25)μ/L,(4277.24 ± 231.35)μ/L,(5842.76 ± 248.18)μ/L] and plasma DAO concentration [(4.23 ± 0.51) μ/L,(5.13 ± 0.32)μ/L,(7.83 ± 0.76) μ/L] in the Model group were significantly higher at 6 h,12 h,24 h postoperation than the Normal group[serum amylase:(2074.11 ± 101.56)μ/L,DAO:(2.32 ± 0.48) μ/L] and Sham group [serum amylase:(1885.27 ± 103.89) μ/L,(1778.57 ± 98.94) μ/L,(1935.24 ± 105.87) μ/L].The levels of serum amylase [(2576.70 ± 123.43) μ/L,(2783.77 ± 155.53) μ/L,(2902.32 ±176.25) μ/L] and plasma DAO concentration (3.59 ± 0.57) μ/L,(3.98 ± 0.67) μ/L,(6.44 ± 0.71) μ/L in the EGCG group were lower than the Model group,but higher than the Normal group and the Sham group.Compared with the Normal group and the Sham group,the concentration of MDA and NO [MDA:(2.28±0.35) μmol/g,(3.02 ± 0.41) μmol/g,(3.78 ± 0.48) μmol/g ; NO:(16.80 ± 0.60) μmol/g,(18.23 ± 0.78) μmol/g,(20.14±0.82) μ,mol/g] significantly increased in the Model group at 6h,12 h,and 24 h postoperation.The concentration of MDA and NO (1.67±0.14) μmol/g,(1.75±0.16) μmol/g,(1.84±0.22) μmol/g; NO:(9.09±0.31) μmol/g,(9.32±0.44)μmol/g,(10.15±0.52)μmol/g were lower in the EGCG group in all times than the Model group.Compared with the Normal group (0.10) and the Sham group (0.25).The expression ofiNOS was significantly increased in the Model group(1.86).The expression of iNOS was lower in the EGCG group (0.66) in all times than the Model group,but higher than the Normal group and the sham group (P＜0.05).Conclusion Conclusion EGCG can protect the damages of intestinal mucosa barrier function in SAP rats,probably through reducing free radicals,and the iNOS expression.

As a newly epidemic, 2019 coronavirus disease (COVID-19) with a concentrated outbreak poses a great challenge to medical treatment. The severe and critical patients are complex complicatied with the psychological problems, and the medical staff are overworked and under tremendous psychological pressure. The surgeon participated in emergency medical rescue could provide professional treatment for the patients combined with surgical diseases, as well as specialized training for the non-surgeon crew, to reduce surgical-related mortality. With the advantages of good team consciousness, strong aseptic concept and good psychological quality, the surgeons can quickly adapt to and carry out rescue work under the premise of good self-protection. Surgeons need to develop critical care management concepts and focus on the critical care support equipment. Some suggestions are put forward for the standardized training of resident surgeons to cultivate compound talents. It is hoped that this article can lead to the thinking of how to participate in the emergency medical rescue of infectious diseases among surgeons and provide some enlightenment for future surgical education.

As a newly epidemic, 2019 coronavirus disease (COVID-19) with a concentrated outbreak poses a great challenge to medical treatment. The severe and critical patients are complex complicatied with the psychological problems, and the medical staff are overworked and under tremendous psychological pressure. The surgeon participated in emergency medical rescue could provide professional treatment for the patients combined with surgical diseases, as well as specialized training for the non-surgeon crew, to reduce surgical-related mortality. With the advantages of good team consciousness, strong aseptic concept and good psychological quality, the surgeons can quickly adapt to and carry out rescue work under the premise of good self-protection. Surgeons need to develop critical care management concepts and focus on the critical care support equipment. Some suggestions are put forward for the standardized training of resident surgeons to cultivate compound talents. It is hoped that this article can lead to the thinking of how to participate in the emergency medical rescue of infectious diseases among surgeons and provide some enlightenment for future surgical education.