BACKGROUNDTo investigate the correlation between BRI gene expression and the metastatic potential in human non small cell lung cancer cell lines.

METHODSBRI gene differential expression was detected between human lung adenocarcinoma cell lines AGZY83-a and Anip973 by RT-PCR and Northern blot. Anip973 was isolated from AGZY83-a with a higher metastatic potential than its parent line. Other 6 human non small cell lung cancer cell lines, A549, TKB-18, SPC-A-1, GLC-82, 95D and PAa, were also detected for the relationship between BRI expression and metastatic potential.

RESULTSThere was a significant difference in BRI gene expression between AGZY83-a and Anip973 cell lines. BRI was overexpressed in Anip973 cells comparing to AGZY83-a cells. Up regulation of BRI gene was also observed in other 6 lung cancer cell lines, and partly correlated with their metastatic potential. Furthermore, there were two mRNA transcripts in the lung cancer cells, in which the 1.6 kb transcript was the major one.

CONCLUSIONSThe up-regulated expression of BRI 1.6 kb mRNA transcript may indicate the formation of metastatic potential of NSCLC. BRI is possibly a metastasis related gene.

OBJECTIVETo search the candidate gene in the development and metastasis of lung adenocarcinoma and shed light on the possible molecular mechanism of the development of lung carcinoma.

METHODSUsing methods of cell culture, reverse transcription-PCR, RH gene mapping and RNA in situ hybridization.

RESULTSThe cDNA fragment named OPB7-1 was mapped at 1p31-1p34 by RH gene mapping method. The fragment sequences obtained from lung cDNA library of normal person and cell line of AGZY83-a were similar in length but showed individual base difference. For OPB7-1, there is a low homogeneity to known gene by analysis in GenBank, but 3 contigs homologous to OPB7-1 were located at chromosome 1(1p31-1p34). Different degrees of expression were noted in tumor tissues from 24 cases of lung carcinoma, however no significant expression was found in their corresponding normal tissues. And high expression was found in the lung tissues of cases with lymph node metastasis.

CONCLUSIONOPB7-1 may be a novel gene. It may be a tumor related gene in occurrence and metastasis of lung carcinoma.

Adenocarcinoma ; genetics ; Animals ; Chromosome Mapping ; Cloning, Molecular ; DNA, Complementary ; genetics ; Gene Expression Regulation, Neoplastic ; Genetic Predisposition to Disease ; genetics ; Humans ; In Situ Hybridization ; Lung Neoplasms ; genetics ; pathology ; RNA, Neoplasm ; genetics ; metabolism ; Radiation Hybrid Mapping ; Rats ; Tumor Cells, Cultured