Objective　To evaluate the anti-tumor effects of NDV and two genes of virus(HN and F) on athymic mice with human adenocarcinoma xenografts, and to investigate the mechanisms of its oncolytic role. Methods　The experimental model of lung adenocarcinoma xenograft was established. The two experimental groups of athymic mice were given intratumoral injections of NDV and plasmids only once, and compared with PBS controls in the same time. Measure the volume of tumors for 5 weeks and make a curve of the volume. These mice were killed after 5 weeks, and the weight of the tumors was measured. The histological and ultrastructral changes were observed by electromicroscope and microscope. Results　After one injection of live NDV and plasmids, the tumor growth was significantly suppressed (The median inhibitory rate was 71.62％ and 79.40％ respectively). The median weight of tumor of mice treated with NDV was remarkably lower than that of mice treated with PBS, and that of the mice treated with plasmids (P＜0.01). 14％ of the control group had liver and lung metastasis of the tumor, but no metastasis was found in the experimental groups. A great quantity of NDV viron budding was found in the NDV group. Conclusion　NDV could replicate in human lung adenocarcinoma xenografts, leading directly to a potent anti-tumor effect after one injection of live NDV. During the oncolytic process, the gene HN and gene F may play an important role.

BACKGROUNDTo investigate the correlation between BRI gene expression and the metastatic potential in human non small cell lung cancer cell lines.

METHODSBRI gene differential expression was detected between human lung adenocarcinoma cell lines AGZY83-a and Anip973 by RT-PCR and Northern blot. Anip973 was isolated from AGZY83-a with a higher metastatic potential than its parent line. Other 6 human non small cell lung cancer cell lines, A549, TKB-18, SPC-A-1, GLC-82, 95D and PAa, were also detected for the relationship between BRI expression and metastatic potential.

RESULTSThere was a significant difference in BRI gene expression between AGZY83-a and Anip973 cell lines. BRI was overexpressed in Anip973 cells comparing to AGZY83-a cells. Up regulation of BRI gene was also observed in other 6 lung cancer cell lines, and partly correlated with their metastatic potential. Furthermore, there were two mRNA transcripts in the lung cancer cells, in which the 1.6 kb transcript was the major one.

CONCLUSIONSThe up-regulated expression of BRI 1.6 kb mRNA transcript may indicate the formation of metastatic potential of NSCLC. BRI is possibly a metastasis related gene.

OBJECTIVETo search the candidate gene in the development and metastasis of lung adenocarcinoma and shed light on the possible molecular mechanism of the development of lung carcinoma.

METHODSUsing methods of cell culture, reverse transcription-PCR, RH gene mapping and RNA in situ hybridization.

RESULTSThe cDNA fragment named OPB7-1 was mapped at 1p31-1p34 by RH gene mapping method. The fragment sequences obtained from lung cDNA library of normal person and cell line of AGZY83-a were similar in length but showed individual base difference. For OPB7-1, there is a low homogeneity to known gene by analysis in GenBank, but 3 contigs homologous to OPB7-1 were located at chromosome 1(1p31-1p34). Different degrees of expression were noted in tumor tissues from 24 cases of lung carcinoma, however no significant expression was found in their corresponding normal tissues. And high expression was found in the lung tissues of cases with lymph node metastasis.

CONCLUSIONOPB7-1 may be a novel gene. It may be a tumor related gene in occurrence and metastasis of lung carcinoma.

Adenocarcinoma ; genetics ; Animals ; Chromosome Mapping ; Cloning, Molecular ; DNA, Complementary ; genetics ; Gene Expression Regulation, Neoplastic ; Genetic Predisposition to Disease ; genetics ; Humans ; In Situ Hybridization ; Lung Neoplasms ; genetics ; pathology ; RNA, Neoplasm ; genetics ; metabolism ; Radiation Hybrid Mapping ; Rats ; Tumor Cells, Cultured