Objective To investigate the effect of serum neuron-specific enolase on prognosis of elderly progressive cerebral infarction ( PCI) .Methods 60 senile patients with PCI were selected as the PCI group,at the same time,60 non-cerebral vascular patients were selected as the control group.The patients in both two groups were given basic therapy.Before treatment,serum NSE values,NIHSS score,PCI group infarct area were measured and the prognosis were followed up at 3 months.Results The serum NSE concentration of PCI group was (23.5 ±4.6)ng/mL, which was significantly higher than (4.2 ±1.6)ng/mL of the control group(t=19.41,P<0.01).In PCI group,the serum NSE and NIHSS score in patients with large area infarction were significantly higher than patients with non-massive cerebral infarction(t=4.991,5.985,all P<0.05);The serum NSE and NIHSS scores were significantly higher in patients with disturbance of consciousness than patients with the unconscious barriers(t=6.695,6.055,all P<0.05);The survival was significantly higher compared (t=8.247,6.465,all P<0.05) after March follow-up, death serum NSE and NIHSS score on admission.Conclusion The detection of serum NSE level in patients with senile PCI is conducive to predict the disease,improve the clinical efficacy of the treatment of cerebral infarction.

Objective:To explore factors related to attempted su ic ide in female residents in rural area.Method:128 attempted suicide women in countryside were assessed with self-made inventory,FES-CV(Family Enviro nment Scale-Chinese Version),Short MMPI,Screener for Mental Disorders.128 norma ls matched with age,sex ect were also received above tests.Results: The main factors related to attempted suicide included conflict in family or a bout marriage(43.8%),neighbor conflict(25.8%).The main methods of suicide wer e self-poisoning, self-drowning,hanging.Compared with control group,the study g roup was obviousl y having less cohesion,culture,active recreation,poorer organization and more family conflicts .MMPI showed suicide attemptors had more anxiety,suspicion,depression and dersi s m.22 of the 128 could be diagnosed as mental disorders(18 depression,3 schizoph r enia,1 dysthymia).Conclusion:Suicide attempt in rural women has rel ation to personality traits,poor family environment and mental disorders.

Objective To explore the effects of antidepressant combined with improved Morita therapy in the treatment of somatoform disorder. Methods 78 patients with somatoform disorder were randomly divided into study group (medication combined with improved Morita therapy,39 cases) and control group (medication,39 cases). Both groups were assessed with symptom checklist 90 (SCL-90), Eysenck personality questionnaire ( EPQ )and global assessment scale ( GAS ) before and after treatment. Results After treatment, the factor scores in SCL-90 of the study group were (1. 14±0.41),(1.81 ±0.44),(1.27 ±0.41),(1.20±0.54),(1.49 ±0. 66 ), ( 1.25 ± 0. 42 ), ( 1. 09 ± 0.48), ( 1.07 ± 0. 42 ), ( 1.16 ± 0. 42 ), and that of the control group were ( 1. 46±0.44) ,(2.19±0.50),(1.56±0.40),(1.54±0.56),(1.85 ±0.48),(1.47±0.44),(1.33±0.54),( 1.38 ± 0. 58 ), ( 1.38 ± 0. 45 ), there were significant differences ( P ＜ 0. 05, P ＜ 0. 01 ). EPQ-E and EPQ-N of the study group were 7.02 ±4.06 and 12.7 ±3.89 respectively ,EPQ-E and EPQ-N of the control group were 14.8± 4.3 and 8.97 ± 4.32 respectively, there were significant differences ( P ＜ 0.05 ). The obvious effective rates of two groups were 82. 05% and 56.41% (x2 =4.875, P＜0. 05 ). Conclusion The effects of antidepressant combined with improved Morita therapy is much better in the treatment of somatoform disorder.

BACKGROUND: Contingent negative variation (CNV) is a kind of cerebral evoked potential, which is closely related with mental medicine and psychology.OBJECTIVE: To investigate the different manifestations between patients with affective disorder and normal adults in CNV detection.DESIGN: Case-control trial.SETTING: Department of Function, Psychiatric Hospital of Zibo, Shandong Province.PARTICIPANTS: Totally 29 inpatients and outpatients including 14 males and 15 females with affective disorder in the Fifth People's Hospital of Zibo between February 2005 and January 2006 were selected. The guardians were all informed and agreed the content; the diagnosed criteria of the patients were accorded wit h the criteria of DSM-Ⅳ. According to their mental status when hospitalized, the patients were divided into manic episode group and depressive episode group. There were 11 cases in the manic episode group, whose scores of Bech-Rataelson Mania Scale (BRMS)＞16; there were 18 cases in the depressive episode group, including unipolar depression (without history of mania episode) and bipolar depression (with history of mania episode) whose Hamilton Depression Scale (HAMD)scores ＞ 24. A total of 27 healthy hospital staff were selectedas control group including 14 males and 13 females aged 21-53 years and the mean age was (32.5±5.9) years. There was no significant difference in the age and sex among the three groups.METHODS: The CNV test was carried out in a soundproof room. Seating on a soft chair, the subject kept vigilant and focused on a fixed point in the front. The platinum electrode of 8 mm was attached to the subject's vertex Cz according to the International 10/20 System, referred to the right mastoid. The middle point of 2 eyebrows was grounded. The CNV waveforms were described by a L2-3 XY Functional Plotter. Standard CNV was composed of light signal and click signal. The flashlight and loudspeaker were located at the right back of the examinee. The subject was asked to press the button with his right finger to cut off the short sound as soon as his ears received the imperative click. It would take 2 500 ms for one CNV trial, and 20 trials as one experiment. Every subject was given the experiment for twice, and the standard amplitude was set by inputting a standard voltage of 20 μV to the same recording system.MAIN OUTCOME MEASURES: The CNV latency, amplitude B, area of negative expectancy wave before command signal, and area of negative change post-imperative negative variation (PINV) latency of the subjects in the two groups.RESULTS: All the 56 patients were involved in the result analysis.Compared with the control group, the mania group showed higher CNV amplitude [(22.8±4.8) μV; (16.0±5.7) μV; P ＜ 0.01], and the depression group presented smaller A-S2 area [(292.8±161.6)μV2; (412.8±159.9) μV2;P ＜ 0.01], and prolonged PINV latency [(261.9±79.3), (211.9±36.3) ms; P＜ 0.05]. The prolonged PINV latency was found in the unipolar depression group compared with the bipolar depression group [(344.5 ±66.9),(230.4±83.8) ms; P ＜ 0.01].CONCLUSION: CNV and PINV suggest that prolonged latency is the trait maker of the patients with unipolar depression, and CNV amplitude changes belong to the state makers of affective disorder.

Objective To explore Naukan therapy and health education combined with drug treatment clinical efficacy in the treatment of alcohol dependence.Methods The 66 cases of male alcohol dependent patients were randomly divided into study group (33 cases) and control group (33 cases),and both of patients were taking generic drugs.The study group was given Naukan treatment (including centralized within concept and dispersion Vipassana),and the control group received health education (including collective health education and individual health education).Duration of treatment was 4 weeks,the total number of hours were 55.5 h,56 h.Obsessive compulsive drinking scale(OCDS),social support rating scale (SSRS),Minnesota multiphasic personality inventory (MMPI) for the two groups before and after treatment; and follow-up the rate of recovery drink three months after hospital discharge.Results ① OCDS score after treatment in the study group was lower than that in the control group,the difference was statistically significant ((48.21 ± 2.05) vs.(54.15 ± 2.01),t =11.885,P＜0.01).②After treatment SSRS total score,objective support,subjective support,social utilization factor scores were higher than those in the control group,and differences were statistically significant ((49.05±5.63)vs.(40.15±6.50),t=5.946,P=0.000) ; ((14.32±3.51) vs.(8.72±6.22),t=4.504,P＜ 0.05) ; (27.02±4.26) vs.(19.45±4.92),t=6.682,P=0.000; (10.02±3.30) vs.(7.52±3.86),t=2.828,P=0.006).③MMPI with schizophrenia (Sc),paranoia (Pa),mental weakness (Pt),psychopathy (Pd) ie 8/6/7/4 model-based in two groups,and Sc,Pa,Pd,Pt dimensions of the original sub in study group were lower than those in the control group after treatment,in addition to outside Pt differences were statistically significant ((25.43 ± 6.76) vs.(29.01 ± 6.72),t =2.158,P=0.035; (14.12±6.01) vs.(18.32±6.42),t=2.744,P=0.008; (18.63±6.85) vs.(23.29±6.12),t=2.342,P=0.022).④Redrinking rate in study group was 39.39％ (11/33),and 72.73％ (24/33)in the control group,the difference was statistically significant(x2 =7.44,P＜0.05).Conclusion The Naukan therapy combined with general drug treatment can solve the problem of patients with alcohol dependenceof alcohol dependence in the psychological and physiological aspects,and its efficacy is superior to general health education combined drug therapy.

Background Research determined that TLR4 is positively expressed on the macrophages in iris and ciliary in acute endotoxin-induced uveitis(EIU),indicating that TLR4 participated in the pathogenesis of the anterior uveitis.Objective The aim of this study is to observe the expressions of toll-like receptor-4(TLR4),Myeloid differentiation factor 88(MyD88),NF-κB p65 in iris tissue in the eyes with endotoxin-induced acute anterior uveitis.Methods Animal models of acute anterior uveitis were established by a hind footpad injection of 200μg Cholera vibrio LPS in 40 SPF Wistar rats with the age of 6-8 weeks.Other 10 age-matched rats were as normal controls.Ocular inflammation was examined under the slit lamp microscope at the 2-hour interval after the injection and intensity of inflammation was scored according to the standard of Lajavardi[4].Histopathology examination was performed for the evaluation of inflammatory reaction of iris and ciliary tissues by HE staining at 24 hours after LPS injection.Expressions of TLR4,MyD88 and NF-κB p65 in iris and ciliary body tissue were detected through immunohistochemistry.TLR4~+,MyD88~+ and NF-κB p65~+ cells were counted.Results The inflammatory reaction was gradually enhanced after injection of LPS and peaked at 24 hours and allivated 48 hours later.The infiltration of lots of inflammatory cells and fibrinous exudate were exhibited in the anterior chamber,posterior chamber,iris and ciliary tissue under the optical microscope at 24 hours after injection of LPS.No positive expressions of TLR4,MyD88 and NF-κB p65 in iris-ciliary body complex were found in normal control rats.The positive cells for TLR4,MyD88 and NF-κB p65 in iris-ciliary body complex were significantly different among 12 hours group,48 hours group and 72 hours groups(F=46.79,P＜0.05;F=54.37,P＜0.05;F=85.32,P＜0.05),and the positive cells for TLR4,MyD88 and NF-κB p65 peaked at 24 hours after injection of LPS.Conclusion The expression of TLR4 and its downstream signal transduction molecules MyD88,NF-κB p65 vary in uvea during EIU,indicating the potential role of LR4-MyD88 dependent pathway in the pathogenesis of acute anterior uveitis.

Objective To explore the functional improvement of dancers' sensorimotor system after years of dance training.Methods Twenty-three dancers(the dance group) of more than 6 years of dancing experience and twenty-one college students (the control group) without any dance experience were recruited in this study.Then the resting state functional magnetic resonance imaging(RS-fMRI) was conducted for both groups and the data were collected.Regional homogeneity (ReHo),fractional amplitude of low frequency fluctuation (fALFF) and functional connectivity (FC) were compared between the two groups.Results Compared to the control group,significant increase was observed in the ReHo of the bilateral postcentral gyruses,the left superior temporal gyrus,the right precentral gyrus and the middle occipital gyrus and fALFF in the bilateral precentral gyruses of the dance group.Moreover,the connection between the right precentral gyrus and left precentral gyrus and the FC between the right precentral gyrus and bilateral postcentral gyrus enhanced significantly in the dance group compared to the control group.Conclusions The functional modulation of dancers' sensorimotor system may be associated with the long-term dancing experience,which may lead to the changes in action perception,memory processing,motor learning and movement control to enhance the formation of the mode to observe,implement and adjust complex actions.Our study has provided a supporting evidence for dancing-induced brain plasticity.

OBJECTIVETo investigate expression of Her-2/neu (c-erbB-2) and its significance in ovarian epithelial tumors.

METHODS106 specimens and clinical history of ovarian epithelial tumors were collected including 54 cases of malignant, 33 cases of borderline and 19 benign cases. There were 19 cases of stages I and II and 35 cases of stages III and IV in ovarian carcinoma and all borderline malignant cases were stage I according to FIGO's standard. Immunohistochemical staining for Her-2/neu was performed by LSAB method.

RESULTSThe positives rates of Her-2/neu of benign, borderline and malignant tumors were 47.4% (9/19), 84.8% (28/33,), and 85.2% (46/54), respectively. Both borderline and malignant cases were found to have higher expression of Her-2/neu than those of benign cases (P < 0.02 and P < 0.01) respectively. Overexpression of Her-2/neu showed in 14/54 (25.9%) of malignant cases, 3/33 (9.1%) of borderline, and none of benign cases. Overexpression of Her-2/neu in malignant tumors cases with metastasis was higher than those without metastasis (P < 0.001).

CONCLUSIONOverexpression of Her-2/neu is associated with biological aggressiveness of ovarian cancer.

Ikaros represents a zinc-finger protein family important for lymphocyte development and certain other physiological processes. The number of family members is large, with alternative splicing producing various additional isoforms from each of the five homologous genes in the family. The functional forms of Ikaros proteins could be even more diverse due to protein-protein interactions readily established between family members. Emerging evidence suggests that targeting Ikaros proteins is feasible and effective in therapeutic applications, although the exact roles of Ikaros proteins remain elusive within the intricate regulatory networks in which they are involved. In this review we collect existing knowledge as to the functions, regulatory pathways, and molecular mechanisms of this family of proteins in an attempt to gain a better understanding through the comparison of activities and interactions among family members.

Morita therapy has been bom for more than 100 years.Inpatient Morita therapy is highly oper-able and easy to master.It can improve many refractory neuroses through four-stage treatment.But more neuroses are treated in outpatient clinics,and Morita therapy cannot be used in hospitalized patients.Therefore,the formula-tion of expert opinions on outpatient operations is particularly important.This paper is based on domestic and for-eign references,and after many discussions by domestic Morita therapy experts,and then drew up the first version of the expert opinions on operation of outpatient Morita therapy.Meanwhile the operation rule of Morita therapy in three stages of outpatient treatment was formulated:in the etiological analysis stage,under the theoretical guidance of Morita therapy,analyze the pathogenic factors,to improve treatment compliance and reduce resistance;during the operating stage,guide patients to engage in constructive and meaningful actions,realizing the achievement of letting nature take its course principle;in the cultivating character and enriching life stage,pay attention to positive infor-mation,expanding the scope and content of actions,improving the ability to adapt to complex life,and preventing recurrence caused by insufficient abilities.It will lay a foundation for the promotion of Morita therapy in domestic outpatient clinics,so that more patients with neurosis and other psychological diseases could receive characteristic Morita therapy treatment in outpatient clinics.