[Objective]To establish a HPLC method or the determination of ginsenside Rg1 in Danqi tablet.[Method]HPLC conditions were as fellows:DiamonsiLTM C18 colum,a mobile phase of MeOH:H2O(48:52),and the wavelength at 280nm.[Results] There was a good linearity within the rang of 4.8ug～24.0ug of ginsenside Rg1.The average recover was 99.80%and RSD was 1.46%.[Conclusion]This method can be used for the quality control of Danqi tablet.

Objective To study the changes of biochemistry indexes in blood serum and bone mineral density (BMD) of secondary osteoporosis after spinal cord injury (SCI) in rats. Methods 120 male Wistar rats were divided randomly into 12 groups (n=10). The rats in control groups were only removed larmina with dura intact, without spinal cord injury at the level of the tenth thoracic vertebrae, the rats in experimental groups were also removed larmina, then paraplegic model was made by the method of Allen's (60gcm). The changes of biochemistry indexes in blood serum and BMD were observed. Results Compared with control groups, the concentrations of alkaline phophatase (ALP) were decreased at the 1st and the 3rd week in experimental groups (P0.05). BMD of tibia was decreased at the 7th and 11th week in experimental group (P

Objective:To explore the effectiveness of self-assisted couple communication program(SACCP)onprenatal depression and marital quality in pregnant women.Methods:Totally 90 pregnant women receiving ante-natal checkup in Air Force Medical Center with the Edinburgh Postnatal Depression Scale(EPDS)scores of ≥10 were voluntarily recruited and randomly allocated in routine caring and SACCP groups.Thirty-nine pregnant women in SACCP group and 37 pregnant women in routine caring group ultimately completed the study.In addition to rou-tine caring,the SACCP group completed a 10-week self-assisted family communication program once a week.The EPDS and Olson Marital Quality Questionnaires(ENRICH)were used as outcomes,and covariance and independ-ent t-teat were adopted to evaluate intervention effectiveness.Statistical analysis was conducted using the full analy-sis set and the per protocol set respectively.Results:Totally 76(84.4％)pregnant women received allocation and end-point revaluation.Covariance analysis revealed that EPDS adjusted means were higher in the SACCP group than in the routine caring group(P＜0.05)in both sets.T-test showed that marital satisfaction and couple communica-tion scores were higher in the SACCP group than in the routine caring group(Ps＜0.01)in the full analysis set.Conclusion:The self-assisted couple communication program(SACCP)could effectively alleviate clinical symptoms of prenatal depression of pregnant women and improve marital quality.

Objective To explore the use of new media technology to estimate the size of the online MSM population in Pudong New Area, to understand the frequency of new media software use, HIV testing and high-risk behavior, and to provide a basis for AIDS prevention and control. Methods The online MSM registration information in Pudong New Area was collected through a gay dating APP. At five time points on March 28 and April 11, 2019, MSM who logged into the gay dating APP were counted. The MSM were confirmed by comparing the login name and registration information. The capture-recapture (C-R) method was used to calculate the MSM population size. Meanwhile, a questionnaire survey was conducted on the online MSM. Results The MSM population size in Pudong New Area was estimated at 13 174 [95% CI: 9,672~16,676] through the C-R method. The proportion of multiple sexual partners accounted for 72.5%. According to the ordered multi-classification logistics analysis, age, education level, experience of attending a talk on AIDS, and experience of receiving AIDS publicity and education in the VCT clinic were the influencing factors of AIDS awareness. Conclusion New media technology provided new technical means for estimating the size of MSM population. The MSM population^’s awareness of AIDS risk and prevention still needs to be strengthened. It is worthy of further studies on the role of new media technology in AIDS prevention and health education in the future.

Nanotechnology-based photothermal therapy has attracted great attention in the past decade. Nevertheless, photothermal therapy has some inherent drawbacks, such as the uneven heat production and limited laser penetration, often leading to insufficient treatment outcomes. Here, we developed a combination strategy to improve cancer therapy. The biomimetic albumin-modified gold nanorods (AuNRs) were prepared with incorporation of paclitaxel (PTX). This therapeutic system was characterized by several features. First, the albumin modification enhanced the biocompatibility and colloidal stability. Second, the surface-coated albumin promoted cellular uptake the albumin-binding protein pathway. Third, PTX was incorporated hydrophobic interaction between PTX and the albumin lipophilic domain. Fourth, the system can be used for combined photothermo-chemotherapy for yielding synergistic effects. The antitumor activity of the system was evaluated both and using the HCT116 colon cancer cell and tumor model. The combination therapy was found with an enhanced treatment efficiency and no obvious side effect. Most importantly, the thermal effect was also discovered with the ability to modulate the tumor microenvironments and suppress the macrophages polarization towards the M2 pro-tumor phenotype. It could be a mechanism for photothermal immunotherapy. The combination strategy and the system provide a potential method for cancer therapy.