Objective To understand the infection status of human parasites among rural residents in Liaoning Province, and provide scientific basis for control of parasites. Methods From October 2014 to December 2015, in 32 counties of 4 different ecological zones of Liaoning Province, survey sites were determined by stratified sampling according to their economic status. More than 250 people were examined at each survey site, and the rate of resident inspection was no less than 85％. The fecal samples of the subjects were collected, intestinal helminth eggs, intestinal protozoa and the eggs of Enerobius vermicularis in children aged 3 - 6 were examined by modified Kato-Katz thick smear, iodine stain direct smear and adhesive cellophane anal swab, respectively. Results Totally 26520 rural residents were surveyed, their infection rate of parasitic disease was 0.29％ (78/26520). Among them, the infection rate of Ascaris and Trichuris was 0.29％ (76/26520) and 0.02％ (5/26520), respectively; Enerobius vermicularis and Hookworm were not detected. The parasite infection rate in the 60-year-old age group was relatively high (0.49％, 34/6939). Conclusion The infection rate of rural residents in Liaoning Province is at a relatively low level, which is closely related to the improved living standard and the continuous improvement of environmental hygiene in recent years.