1.Expression of novel apoptosis-related protein PDCD5 in granulosa cells of polysystic ovary syndrome
Chunling SUN ; Jie QIAO ; Zhenxing HU ; Ting ZHANG ; Yingyu CHEN
Journal of Peking University(Health Sciences) 2003;0(05):-
Objective: To detect the novel apoptosis-related protein PDCD5 expression in granulosa cells of polysystic ovary syndrome(PCOS) and normal ovary, and explore the pathogenesis of PCOS. Methods:The granulosa cells were collected from 30 cases of PCOS and normal ovary in IVF-ET. Expression of PDCD5 was detected by flow cytometry; immunofluorescence and immunohistochemistry. Cell apoptosis was detected by Propidium Iodide (PI) staining. Results: The number of hypodiploidy cells associated with apoptosis in granulosa cells of PCOS was greater than that of the normal control. PDCD5 protein expression in PCOS granulosa cells was significantly higher than that in normal ovary(P
2.Nuclear translocation of apoptosis-related protein TFAR19 in TF-1 cells undergoing apoptosis
Yingyu CHEN ; Ronghua SUN ; Wenling HAN ; Yingmei ZHANG ; Quansheng SONG ; Chunhui DI ; Dalong MA
Journal of Peking University(Health Sciences) 2001;33(2):97-100
Objective: To study the expression and localization of apoptosis-related protein TFAR19 in TF-1 cells undergoing apoptosis. Methods: Using monoclonal antibody against TFAR19, the expression level and cell localization of TFAR19 were examined by fluorescence microscope, confocal laser scan microscope(CLSM) and flow cytometry. Simultaneously, we also analyzed the relationship of TFAR19 protein with phosphatidylserine (PS) externalization and cell nuclear DNA fragmentation. Results: The level of TFAR19 proteins expressed in TF-1 cells treated with GM-CSF withdrawal was significantly increased compared with normal TF-1 cells, then translocated rapidly from cytoplasm to the nucleus of cells. Appearance of TFAR19 in the nucleus of apoptotic cells preceded the detection of PS externalization and DNA fragmentation. Conclusion: Nuclear translocation of TFAR19 protein is one of the earliest events of cell apoptotic process. These data provided a new clue to further approach to the biological function of TFAR19 and study of cell apoptosis.
3.Apoptosis-associated Gene PDCD5 Expression in Esophageal Cancer in Xinjiang Kazakh and Han Nationality
Hui GUO ; Jianbing DING ; Wei SUN ; Xiumin MA ; Tong ZHANG ; Cunbao CAO ; Yingyu CHEN
Chinese Journal of Clinical Oncology 2010;37(4):213-215
Objective: To study the expression of PDCD5 mRNA and its significance in esophageal cancer in Xinjiang Kazakh and Han nationality. Methods: RT-PCR was used to detect the mRNA of PDCD5 in 40 cases of esophageal cancer (18 cases of Kazakh, 22 cases of Han). Results: The positive rate of PDCD5 mRNA expression in 40 samples of esopha-geal cancer tissue, adjacent tissue, and normal tissue was 80.0% (32/40), 80.0% (32/40) and 87.5% (35/40), respectively, with no significant difference (P>0.05). The Ods of cancer tissues, adjacent tissues, and normal tissues were 0.7644± 0.1444, 0.9341 ±0.1631 and 1.8703±0.4767, respectively. The expression of PDCD5 was significantly increased in cancer tissues compared with that in normal tissues (P<0.05). The expression level of PDCD5 mRNA was not significantly correlat-ed with the degree of differentiation and lymph node metastasis. Conclusion: No significant difference was found in PDCD5 mRNA expression in esophageal cancer between Xinjiang kazakh and Han nationality (P>0.05). The expression of PDCD5 is not correlated with the degree of differentiation, depth of invasion or lymph node metastasis. Detection of PDCD5 mRNA expression in esophageal cancer tissues may provide valuable information for patient prognosis.
4.The application of three-dimensional cell culture technology in the field of cancer research
Yingyu WANG ; Yin LU ; Weiwei ZHANG ; Zhiguang SUN ; Shizhong ZHENG ; Lei CHEN
Chinese Pharmacological Bulletin 2009;25(12):1545-1548
Traditional 2D cell culture technology is widely used in cell culture field, but it lacks the formation of 3D matrix scaffold and lacks specific cytokines in vivo.Cells in 3D cell culture system are similar to cells in vivo for gene expression and cell activity. The article focuses on the application of three-dimensional cell culture technology in cancer research, including the construction of tumor microenvironment,tumor biological behavior, tumor angiogenesis, drug resistance and so on and provides a reference for cancer research workers.
5.The role of TF-1 cell apoptosis-related gene 19 in systemic lupus erythematosus
Qinghua SONG ; Jing WANG ; Yingyu CHEN ; Chunhui DI ; Ronghua SUN ; Xuerong CHEN ; Shiyi LI
Chinese Journal of Pathophysiology 1986;0(02):-
AIM: To clarify the role of new apoptosis-related gene, TF-1 cell apoptosis-related gene 19(TFAR19), in the pathogenesis of systemic lupus erythematosis (SLE) and the relationship between TFAR19 and SLE. METHODS: DNA Ladder detection, Western blotting, immunological fluorescence method, ELISA and so on were used to test if ultraviolet B(UVB) could induce HaCaT cell apoptosis and TFAR19 expression. RESULTS: HaCaT cell apoptosis could be detected after 24 hours of 30 mj/cm 2 UVB irradiation. Also, we found that in active SLE patients, the TFAR19 antibody was increased, but not significant compared to the normal control. CONCLUSION: TFAR 19 is involved in the process of UVB induced ketatinocyte line HaCaT apoptosis and SLE pathogenesis.
6.A Novel Mutation p.L461P in KRT5 Causing Localized Epidermolysis Bullosa Simplex
Xin JIANG ; Yingyu ZHU ; Huihui SUN ; Feng GU
Annals of Dermatology 2021;33(1):11-17
Background:
Epidermolysis bullosa (EB) is a rare genetic disease with widely different clinical manifestations, but the relationship between genotype and phenotype is not fully understood. In the present study, we recruited a Chinese family in which two members had been diagnosed with localized EB simplex (EBS), with clinical manifestation, including blisters and erosions on the soles of the feet since infancy.
Objective:
To identify and confirm the genetic variation in a Chinese family diagnosed as localized EBS.
Methods:
Our study included two patients, other healthy members of the family, and 100 normal controls. Genomic DNA samples were isolated from each participant, and then polymerase chain reaction (PCR) direct sequencing was performed.
Results:
The results of PCR direct sequencing revealed a novel heterozygous missense mutation in codon 461 of exon 7 of KRT5 (c.1382T>C), which led to an amino acid change (p.L461P) in the patients with EBS but was absent in unaffected family members and 100 unrelated control samples.
Conclusion
The present study broadens the mutational spectrum of EBS, and this knowledge could be harnessed for prenatal screening, gene diagnosis, and gene therapy for lo-calized EBS.
7.Comparison of surveillance efficacy between black box and light trap methods
Yingyu YANG ; Chunwei SUN ; Jiang ZHU
Shanghai Journal of Preventive Medicine 2024;36(7):697-700
ObjectiveTo evaluate the field mosquito surveillance efficacy between the black box method and the light trap method. MethodsEight light traps and eight black boxes were placed in each of the eight field sites in Baoshan District of Shanghai, with a minimum distance of 10 meters between each traps. Surveillance was conducted twice a month from May to September 2023. ResultsThe total number of mosquitoes (273) and female mosquitoes (228) captured by the black box method were 0.795 and 0.774 times higher than those caught by the light trap method, respectively. There was no statistically significant difference (P>0.05) between the mosquito density (t=1.723 5, P=0.100 2) and the female mosquito density (t=1.805 9, P=0.101 1), and there was a correlation (P<0.001) between the mosquito density (r=0.942 7) and the female mosquito density (r=0.896 5). There were no significant differences in the composition ratio of mosquito species(χ2=0.100 1, P>0.05) and female mosquitoes(χ2=0.394 4, P>0.05) captured by black box and light trap methods. In four different habitats, the composition ratios of mosquito species captured by the black box and light trap methods were: hospitals (38.10% and 38.77%) > rural housing areas (36.26% and 37.61%) > parks (14.65% and 12.54%)> urban housing areas (10.99% and 11.08%), and the composition ratios of female mosquitoes captured by the black box and light trap methods were: rural housing areas (40.35% and 42.52%) > hospitals (37.72% and 36.05%) > parks (13.16% and 13.27%) > urban housing areas (8.77% and 8.16%). In different habitats, there was no significant difference in the composition ratio of mosquitoes captured by two monitoring methods(χ2=0.5987, P>0.05), and there was no significant difference in the composition ratio of female mosquitoes(χ2=0.2993, P>0.05). ConclusionWith widen application and fewer requirements for equipment, operation skills, and use cost the black box method can be interchanged with the light trap method for monitoring mosquito density, especially in disaster areas or remote areas with difficult in having access to electricity.
8.Multicenter study on the etiology characteristics of neonatal purulent meningitis
Yanli LIU ; Jiaojiao CAI ; Xiaoyi ZHANG ; Minli ZHU ; Zhenlang LIN ; Yicong PAN ; Junhu ZHENG ; Yiwei ZHAO ; Xiang WANG ; Hongping LU ; Meifang LIN ; Ji WANG ; Haihong GU ; Lizhen WANG ; Keping CHENG ; Yuxuan DAI ; Yuan GAO ; Junsheng LI ; Hongxia FANG ; Na SUN ; Lihua LI ; Xiaoquan LI ; Ying LIU ; Yingyu LI ; Wa GAO ; Minxia LI
Chinese Journal of Infectious Diseases 2023;41(6):393-400
Objective:To study the distribution and antibiotics resistance of the main pathogens of neonatal purulent meningitis in different regions of China.Methods:A retrospective descriptive clinical epidemiological study was conducted in children with neonatal purulent meningitis which admitted to 18 tertiary hospitals in different regions of China between January 2015 to December 2019. The test results of blood and cerebrospinal fluid, and drug sensitivity test results of the main pathogens were collected. The distributions of pathogenic bacteria in children with neonatal purulent meningitis in preterm and term infants, early and late onset infants, in Zhejiang Province and other regions outside Zhejiang Province, and in Wenzhou region and other regions of Zhejiang Province were analyzed. The chi-square test was used for statistical analysis.Results:A total of 210 neonatal purulent meningitis cases were collected. The common pathogens were Escherichia coli ( E. coli)(41.4%(87/210)) and Streptococcus agalactiae ( S. agalactiae)(27.1%(57/210)). The proportion of Gram-negative bacteria in preterm infants (77.6%(45/58)) with neonatal purulent meningitis was higher than that in term infants (47.4%(72/152)), and the difference was statistically significant ( χ2=15.54, P=0.001). There were no significant differences in the constituent ratios of E. coli (36.5%(31/85) vs 44.8%(56/125)) and S. agalactiae (24.7%(21/85) vs 28.8%(36/125)) between early onset and late onset cases (both P>0.05). The most common pathogen was E. coli in different regions, with 46.7%(64/137) in Zhejiang Province and 31.5%(23/73) in other regions outside Zhejiang Province. In Zhejiang Province, S. agalactiae was detected in 49 out of 137 cases (35.8%), which was significantly higher than other regions outside Zhejiang Province (11.0%(8/73)). The proportions of Klebsiella pneumoniae, and coagulase-negative Staphylococcus in other regions outside Zhejiang Province (17.8%(13/73) and 16.4%(12/73)) were both higher than those in Zhejiang Province (2.9%(4/137) and 5.1%(7/137)). The differences were all statistically significant ( χ2=14.82, 12.26 and 7.43, respectively, all P<0.05). The proportion of Gram-positive bacteria in Wenzhou City (60.8%(31/51)) was higher than that in other regions in Zhejiang Province (38.4%(33/86)), and the difference was statistically significant ( χ2=6.46, P=0.011). E. coli was sensitive to meropenem (0/45), and 74.4%(32/43) of them were resistant to ampicillin. E. coli had different degrees of resistance to other common cephalosporins, among which, cefotaxime had the highest resistance rate of 41.8%(23/55), followed by ceftriaxone (32.4%(23/71)). S. agalactiae was sensitive to penicillin, vancomycin and linezolid. Conclusions:The composition ratios of pathogenic bacteria of neonatal purulent meningitis are different in different regions of China. The most common pathogen is E. coli, which is sensitive to meropenem, while it has different degrees of resistance to other common cephalosporins, especially to cefotaxime.
9. The effect of peroxiredoxin 2 on transforming growth factor-β1-induced fibroblast proliferation and collagen synthesis
Zhao ZHANG ; Yingyu LIU ; Yan LIU ; Qian LI ; Tingting LIANG ; Fan HONG ; Li FENG ; Ying SUN
Chinese Journal of Industrial Hygiene and Occupational Diseases 2020;38(1):7-12
Objective:
To investigate the effect of peroxiredoxin 2 (Prx2) overexpression on fibroblast proliferation and collagen synthesis induced by transforming growth factor-β1 (TGF-β1) .
Methods:
Fibroblasts were randomly divided into control group (DMEM medium) , TGF-β1 group (5 μg/L TGF-β1) , negative control group (treated with 5 μg/L TGF-β1 and transfected with empty lentiviral vector) , and Prx2 group (treated with 5 μg/L TGF-β1 and transfected with Prx2 overexpression lentiviral vector) . MTT assay was used to measure cell proliferation, immunofluorescence assay was used to measure the expression of 8-OHdG, and Western blot was used to measure the expression of p-JNK, p-P38, collagen type I, collagen type III, and Prx2. SPSS 18.0 was used for statistical analysis. The continuous data were expressed as mean±standard deviation; an analysis of variance was used for comparison between groups, and the least significant difference
10.Human hair follicle-derived mesenchymal stem cells promote tendon repair in a rabbit Achilles tendinopathy model.
Yingyu MA ; Zhiwei LIN ; Xiaoyi CHEN ; Xin ZHAO ; Yi SUN ; Ji WANG ; Xiaozhou MOU ; Hai ZOU ; Jinyang CHEN
Chinese Medical Journal 2023;136(9):1089-1097
BACKGROUND:
Hair follicles are easily accessible and contain stem cells with different developmental origins, including mesenchymal stem cells (MSCs), that consequently reveal the potential of human hair follicle (hHF)-derived MSCs in repair and regeneration. However, the role of hHF-MSCs in Achilles tendinopathy (AT) remains unclear. The present study investigated the effects of hHF-MSCs on Achilles tendon repair in rabbits.
METHODS:
First, we extracted and characterized hHF-MSCs. Then, a rabbit tendinopathy model was constructed to analyze the ability of hHF-MSCs to promote repair in vivo . Anatomical observation and pathological and biomechanical analyses were performed to determine the effect of hHF-MSCs on AT, and quantitative real-time polymerase chain reaction, enzyme-linked immunosorbent assay, and immunohistochemical staining were performed to explore the molecular mechanisms through which hHF-MSCs affects AT. Furthermore, statistical analyses were performed using independent sample t test, one-way analysis of variance (ANOVA), and one-way repeated measures multivariate ANOVA as appropriate.
RESULTS:
Flow cytometry, a trilineage-induced differentiation test, confirmed that hHF-derived stem cells were derived from MSCs. The effect of hHF-MSCs on AT revealed that the Achilles tendon was anatomically healthy, as well as the maximum load carried by the Achilles tendon and hydroxyproline proteomic levels were increased. Moreover, collagen I and III were upregulated in rabbit AT treated with hHF-MSCs (compared with AT group; P < 0.05). Analysis of the molecular mechanisms revealed that hHF-MSCs promoted collagen fiber regeneration, possibly through Tenascin-C (TNC) upregulation and matrix metalloproteinase (MMP)-9 downregulation.
CONCLUSIONS
hHF-MSCs can be a treatment modality to promote AT repair in rabbits by upregulating collagen I and III. Further analysis revealed that treatment of AT using hHF-MSCs promoted the regeneration of collagen fiber, possibly because of upregulation of TNC and downregulation of MMP-9, thus suggesting that hHF-MSCs are more promising for AT.
Animals
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Humans
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Rabbits
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Hair Follicle
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Achilles Tendon/pathology*
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Tendinopathy/pathology*
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Proteomics
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Collagen Type I
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Mesenchymal Stem Cells