Objective:To investigate the value of magnetic resonance imaging, CT and X ray radiography on preoperative diagnosis of osteoporotic vertebral compression fracture and post-operation follow up of percutaneous kyphoplasty.Methods: A retrospectively analysis was adopted for 41 patients of osteoporotic vertebral compression fracture, who were detected by X ray, MRI examination and CT analyze, respectively. And the values of 3 methods for preoperative diagnosis of osteoporotic vertebral fractures and X-ray in postoperative follow-up were analyzed. Results: For preoperative diagnosis of osteoporotic vertebral compression fractures, the accuracy rate of MRI, X-ray and CT were 100%(41/41), 65.85%(27/41) and 92.85%(13/14), respectively. Both of the accuracy rates of MRI and CT were higher than X-ray, and the differences were statistical significant (x2=16.882,x2=3.837;P<0.05); the difference of accuracy rate between MRI and CT was not significant (x2=2.983,P>0.05). After the third day of post-operative and during last follow up, anterior vertebral height and middle vertebral height were significantly higher than before operation (F=17.094,F=15.526,P<0.05); and the Cobb angle and the VAS score of lumbar back pain were lower than before operation, the difference was statistical significant (F=41.085,F=107.338;P<0.05).Conclusion: For preoperative diagnosis of osteoporotic vertebral compression fractures, the accuracy rate of MRI is the highest and X-ray is lowest. X-ray can be used to evaluate surgery effect during postoperative follow-up.

Objective:To investigate the impact of butylphthalide on homocysteine, CRP and nerve function in the patients with a-cute progressive cerebral infarction. Methods:Totally 122 cases of patients with acute cerebral infarction were randomly divided into the observation group (n=60) and the control group (n=62). The control group was treated with the routine treatment, and the ob-servation group was treated with butylphthalide additionally. The treatment course was two weeks. The total effective rate, blood Hcy, the level of plasma CRP and the changes of neurological function deficits scale in both groups before and after the treatment were ob-served and compared. Results:The overall response rate in the observation group (81. 67%) was significantly higher than that in the control group (64. 52%). After the treatment, the blood Hcy and CRP in both groups were significantly lower than that before the treatment(P<0. 05), and that of the observation group was significantly lower than that of the control group(P<0. 05). The neuro-logical function deficits scale in both groups were significantly lower than that before the treatment(P<0. 05), and that of the observa-tion group was significantly lower than that of the control group at the same period. The two groups during the treatment showed no sig-nificant adverse reactions. Conclusion: The effect of butylphthalide in the patients with acute cerebral infarction is effective, which can reduce blood Hcy, the level of plasma CRP and the neurological function deficits scale, and improve the neurological function with-out obvious adverse reactions during the treatment course.

Objective To explore the effectiveness of using middle-latency somatosensory evoked potentials (MLSEP) to predict the prognosis in patients with acute severe stroke. Methods MLSEP, shortlatency somatosensory evoked potentials (SLSEP), and Glasgow Coma Scale (GCS) were recorded in 70 acute severe supratentorial stroke patients within 1 week after onset. All patients were evaluated with modified Rankin Scale (mRS) and follow-up in 6 months after onset. SLSEP and MLSEP were recorded in 20 normal controls. The consistency between MLSEP, SLSEP, GCS and prognosis, as well as the prognostic authenticity of MLSEP, SLSEP, and GCS were analyzed. Results Bilateral N20, N35, and N60 exited in all normal controls. Some waves of MLSEP were absent in stroke patients, and the proportion of absent waves in ipsilateral MLSEP was higher than in contralateral MLSEP. The consistency between bilateral absence of N60 and unfavorable outcome ( Kappa = 0.828, P ＜ 0.01 ), and between bilateral absence of N60 and death ( Kappa = 0.686, P ＜ 0.01 ) was satisfactory. By using the prognostic authenticity analysis of predictors, the ipsilateral absence of N60 showed the highest sensitivity ( 100% ) for unfavorable outcome and death, which added 14.3% compared with the sensitivity of ipsilateral absence of N20 ( 85.7% ). Bilateral absence of N60 showed a high specificity of 100% for unfavorable outcome, which equaled bilateral absence of N20.However, it showed a lower specificity ( 82.9% ) for death, than bilateral absence of N20 (97.1% ).Conclusions MLSEP was able to reflect the degree of brain injury and showed higher sensitivity than SLSEP for predicting unfavorable outcomes. Therefore combined use of MLSEP and SLSEP in evaluating and predicting the outcomes in brain injuries is suggested.

Objective to observe the efficacy and application value of PFNA and Gamma in the treatment of intertrochanteric fractures in elderly. Methods 100 patients with intertrochanteric fractures were divided into PFNA group and Gamma group, who received PFNA treatment and Gamma treatment respectively. And the operation status of patients at different ages,postoperative recovery,and complications were observed. Results The operation time,intraoperatve blood soss of PFNA group were lower than those of Gamma group(P<0. 05). There was no significant difference in fracture healing time. The Harris score of the over 75s in PFNA group was higher than that in Gamma group(P<0. 05),and the complications were less than Gamma group(P<0. 05). Conclusion PFNA was suitable for the patients at differ-ent ages with the advantages of more rigid fixation,good anti-rotation,fewer trauma and fewer complications.

Bacterial extracellular metalloproteases ( BEMPs) are a large group of metal ion-contai-ning proteases. All BEMPs identified so far are endopeptidase or endoprotease. BEMPs can be classified into nine metalloprotease families based on the sequences and structures of enzymatic molecules. Double-valence zinc ion ( Zn2+) is necessarily required by catalytic centers of most BEMPs. The main function of BEMPs in non-pathogenic heterotrophic bacteria is to hydrolyze environmental proteins and polypeptides to provide vari-ous amino acids as nutrients. However, BEMPs of pathogenic bacteria, serving as important virulence fac-tors, help the pathogens invade into hosts and spread in hosts. In recent years, the roles and mechanism of BEMPs in bacterial pathogenesis have attracted great attention. Here, we make a brief review about the structures and types as well as the functions and pathogenic roles of BEMPs.

With the development of aging population,the rational drug use in old people was paid more attention nowadays. So far, a number of studies in Europe, Asia and North America showed that lots of prescriptions with potential irrational drug use could be found out using STOPP & START criteria. In China, there is no systematic tool for guiding the rational drug use in old people yet, therefore, we can learn the content of STOPP&START criteria and use them for reference to promote the rational drug use in Chinese old people and lay the foundation for establishing rational drug use judgment criteria suitable for Chinese old people.

Objective To analyze the DCE-MRI features and ADC value in triple-negative breast cancer and HER-2 overexpres-sion breast cancer,and to improve the j udgment of both characteristic in MRI.Methods To analyse the DCE-MRI imaging of TNBC and HER-2 overexpression breast cancer,measured the ADC value and compared the statistics.Results Compared with the shape, margin,the way of enhancement and ADC value of the TNBC and HER-2 overexpression breast cancer,the distinction has statisti-cally significant(P<0.05);there were no statistically significant difference among age,mass or nomass,categories of lesions and TIC,(P>0.05).Conclusion DCE-MRI features of the shape,margin,the way of enhancement and ADC values were useful for the diagnosis of TNBC and HER-2 overexpression breast cancer.

Objective To perform the cytogenetic diagnosis on single lymphocyte of DMD patient with dystrophin gene exon 50 deletion.Methods Single lymphocytes of a DMD patient with dystrophin gene exon 50 deletion and normal volunteers were picked out and prepared for two-time duplex PCR.Results The rate of precise positive was 92%,91% and 93% in specimens of the patient(SRY positive,exon 50 negative),the male volunteer(SRY positive,exon 50 positive)and the female volunteer(SRY negative,exon 50 positive),respectively.Conclusion Two-time duplex PCR is fit for the genetic diagnosis of single lymphocyte from DMD patient with dystrophin gene exon 50 deletion.

Objective To investigate and analyze clinical analysis of low molecular weight heparin calcium combined with psychological intervention in the treatment of diabetic foot patients. Methods 100 patients with type 2 diabetic foot who were treated in our hospital from February 2015 to November 2016 were selected and randomly divided into the control group and the experimental group, with 50 patients in each group. The patients in the control group and the experimental group were treated with anti infection therapy and insulin hypoglycemic therapy, and the dressing change was carried out. The experimental group was given subcutaneous injection of low molecular weight heparin for psychological intervention, pay attention to the patient's psychological status, strengthen communication with patients, and increase the patient's confidence and treatment compliance. The clinical indexes of the experimental group and the control group were compared and analyzed. Results After the corresponding treatment, 6 cases in the experimental group the wound healing is invalid. The number of ineffective wounds in the control group was 20. The effective rate of treatment in the experimental group was 88.0％, which was significantly higher than that in the control group, and the effective rate was 60.0％, with statistical difference (P<0.05). After treatment, the plasma viscosity and the blood flow velocity of the dorsalis pedis artery in the experimental group were significantly better than those in the control group, with statistical difference (P<0.05). Conclusion Low molecular weight heparin calcium combined with psychological intervention in the treatment of diabetic foot has a good clinical effect, and has the significance of promotion.

OBJECTIVEThis study aimed to improve students' ability in practical and theoretical courses of oral health education and to promote students' learning interest and initiative.

METHODSFourth-year students of the oral medical profession from 2006 to 2008 at Weifang Medical University were chosen as research objects for oral health education to explore the experimental teaching reform. The students were divided into test and control groups, with the test group using the "speak out" way of teaching and the control group using the traditional teaching method. Results of after-class evaluation of the test group, as well as final examination and practice examination of the two groups, were analyzed and compared.

RESULTSAfter-class evaluation results of the test group showed that the "speak out" teaching method was recognized by the students and improved students' ability to understand oral health education. The final examination and practice examination results showed that the score of the test group was higher than that of the control group (P < 0.01).

CONCLUSION"Speak out" teaching methods can improve students' ability for oral health education, in accordance with the trend of teaching reform.