Hippocampus is an important structure of neural memory circuit , which plays an important role in learning and memory integration in human and animal .Vision is the most important channel for people to accept information , while the vision system is the most common organ contacting information from outside .The retina as an important part of the visual system , plays a key role in human cognition of the objective world .The study found that many of pathological changes of the body will make the patients cognitive function decline , but molecular mechanism remains unclear .With the research on cognitive dysfunction was penetrated , the link be-tween how VEGF , PKC expression in hippocampus and retinal tissue and cognitive dysfunction was reviewed in this article .

Spleen and Stomach is the central embodiment of Li Dongyuan's academic thought, which has an extensive and profound influence on the later physicians and clinical application. In this paper,based on the specific Herbal Prescription of Li Dong yuan’s Treatise on Spleen and Stomach,the use of the concept of wholism in TCM,as wel as the Thinking Characteristics of Syndrome Differentiation Dependent Therapy ,discussing the drug administer-ing characteristics of Spleen and Stomach in four aspects:the use of wind drugs,combined ascend and descend,adjusting the medication according to the climatic change of the four seasons,emphasizing dietetic therapy ,medicine and food have the same effect.Obtaining the fol owing conclusions :The Dong Yuan treatment of disease is good at starting from the spleen and stomach. Attach importance to the function of the spleen and stomach of qi activity,its legislation is rigorous, and the thought of harmony between man and nature,ect.The research is helpful to further study of Dong yuan ’s academic thought ,to provide a theoretical basis for treating spleen and stomach disease nowadays.

BACKGROUND: Most cases of early-onset familial Alzheimer disease (AD) are associated with presenilin-1 mutation, which mainly occurs in the 5th and 8th exons. Except for the onset age and family history, sporadic AD is basically the same as early-onset familial AD regarding clinical manifestations and pathological characteristics. Is its pathogenesis also related to the mutation of presenilin-1 gene?OBJECTIVE: To probe into the relationship between sporadic AD and presenilin-1 mutation.DESIGN: Case control analysis based on clinical diagnosis.SETTING: Neurology Department of the First Hospital Affiliated to Sun Yat-sen University.PARTICIPANTS: Outpatients who came to the Memory Obstacle Clinic of the First Hospital Affiliated to Sun Yat-sen University between April 1998 and June 2000 but without familial history of AD were selected and divided into 3 groups: [1] AD group: There were 68 cases confirmed as probable having AD according to the clinical diagnostic standard of NINCDS-ADRDA. [2] Vascular dimentia (VaD) group: There were 25 cases confirmed as probable having vascular dimentia according to the clinical diagnostic standard of NINCDS-ADRDA. [3] Normal control group: There were 20 healthy elders (spouses of some patients in the above two groups)without dimentia.METHODS: The conventional phenol extraction method was used to withdraw DNA from peripheral blood, and polymerase chain reaction-single strand conformation polymorphism analysis (PCR-SCCP) was applied to detect the mutation of the 5th and 8th exons in presenilin-1 gene.RESULTS: All the 5th and 8th exons in presenilin-1 gene were proved to be composed of two single strands and a double strand by PCR-SCCP analysis.No abnormal electrophoresis was found, suggesting no that mutation occurred.CONCLUSION: Sporadic AD differs from familial AD in pathogenicity;mutation does not occur or seldom occurs in the 5th and 8th exons in presenilin-1 gene, thereby cannot be considered as an important causive factor for sporadic AD.

OBJECTIVE:To observe the effect of ondansetron on the anesthesia of cesarean section surgery with spinal-epidur-al anesthesia. METHODS:A total of 60 singletons full-term pregnancy were randomly divided into test group and control group. Test group was given 6% Hetastarch(130/0.4)electrolyte injection 500 ml by intravenous infusion 30 min before anesthesia,and Ondansetreon hydrochloric acid injection 4 ml by intravenous infusion 5 min before anesthesia;control group was given 6% Hetas-tarch(130/0.4)electrolyte injection 500 ml by intravenous infusion 30 min before anesthesia,and 0.9% Sodium chloride injection 4 ml by intravenous infusion 5 min before anesthesia. The clinic data was recorded,including the mean arterial pressure (MAP) and heart rate(HR)before anesthesia puncture(T1),after anesthesia maternal left side(T2),after fetal childbirth(T3)and at the end of surgery(T4),Apgar score and incidence of adverse reactions. RESULTS:MAP in control group at T2,T3 was obviously low-er than T1 and test group,HR was obviously higher than T1 and test group,the difference was statistically significant(P<0.05);there were no significant differences in the MAP and HR in test group at each time point(P>0.05). Apgar score of newborn after 1 min birth in test group was significantly higher than control group,the difference was statistically significant (P<0.05),and there was no significant difference in the Apgar score of newborn after 5 min birth between 2 groups(P>0.05). The ADR incidence in test group was significantly lower than control group(P<0.05). CONCLUSIONS:Ondansetron can effectively reduce the inci-dences of vomit vomitting and hypotension in on the cesarean section surgery with spinal-epidural anesthesia,with good safety.

Objective:To investigate the sensitivity of novel small molecule tyrosine kinase inhibitors NXGH and NXGF to 4 lung cancer cell lines with different EGFR expression and mutant status.Methods:Novel small molecule tyrosine kinase inhibitors NXGH and NXGF based on NXG structure were designed.Four lung cancer cell lines with different EGFR expression and mutation status:PC9(Exon 19 deletion mutation),H1975(L858R mutation combined T790M mutation),H358(wide EGFR expression) and H520(EGFR negative expression) were chosen.Inhibition ratio of NXGH and NXGF at different concentration (1.25,2.5,5.0,10,20,30,40,60,80 μmoL·L-1)against 4 lung cancer cell lines in 48 h were investigated by MTT method.IC50 and cell viability were calculated,and sensitivity between different cell lines were compared.Results:IC50 of PC9,H358,520 and H1975 cells incubated with NXGH were 0.675 μ moL·L-1,12.097 μmoL·L-1,11.368 μmoL·L-1 and 0.981 μmoL·L-1,respectively.IC50 of PC9 and H1975 were less than H358 and H520 when the concentration was 1.25,2.5 and 5 μmoL ·L-1 (P＜0.05).IC50 of PC9,H358,H520 and H1975 cells incubated with NXGF were0.685 μmoL·L-1,4.265 μmoL·L-1,3.097 μmoL·L-1 and 0.331 μmoL·L-1,respectively.IC50 of PC9 and H1975 were less than H358 and H520 when the concentration was 1.25 and 5 μmoL·L-1 (P＜0.05).Conclusion:The novel small molecule tyrosine kinase inhibitors NXGH and NXGF,which were designed and constructed in our laboratory successfully,had high affinity for lung cancer cells with different EGFR expression and mutation status.And they were more sensitive to EGFR mutant cell at low concentration as expected.

Objective To detect the correlations of miR -106 a expression with clinical features of pa-tients with colon cancer ,and to explore the significance of miR -106 a as a prognostic factor .Methods One hun-dred and five patients with primary colon cancer were retrospectively enrolled in this study including their clinical information and slices of FFPE.miR-106a expression was detected by qRT -PCR,and analyzed the correlation between the level of miR -106a and clinical features and survival .Results The level of miR -106a in tumor tissue was higher than adjacent normal tissue (1.142 vs.0.685,P<0.001).miR-106a was correlated with TNM stage and lymphnode metastasis .The higher miR -106a expression,the poorer survival patients were .The hazard risk was increased 3.390 folds(95%CI for HR:1.028 ~11.178)when compared high expression group with the low.Conclusion The aberrant expression of miR -106a may be associated with colon cancer .It will po-tentially be a therapeutic target and prognostic factors .

Aim: To construct a prokaryotic expression vector carrying NuBCP-9-tumstatin(74-98) (abbreviated as NT) gene and to obtain the fusion peptide with antitumor activity. Methods: Nucleotide sequences of antitumor peptides, NuBCP-9 and Tumstatin( 74-98), were connected via a linker(G_4S)_3 based on biased codons of E. coli the fused NT gene was reconstructed using SOE PCR, and inserted into pET32a(+) vector, and transformed in E. coli BL21(DE3). After expression, the novel fusion peptide was purified through nickel-affinity chromatogra-phy, Factor Xa digestion and ultrafiltration. Biological activity of the fusion peptide on ECV304 and A549 cells was evaluated by MTT assay. Results: A prokaryotic expression system with NT gene was successfully constructed. The soluble fusion peptide was accounted for approximately 25% when induced by 0. 5 mmol/L IPTG at 30 ℃ for 4 h. The purified fusion peptide could inhibit cell growth of ECV304 and A549 with inhibition rates of 60. 8% and 65. 2% at 20 μmol/L, respectively. Conclusion: A novel fusion peptide with antitumor activity was cloned, expressed and purified.

Diabetic cognitive impairment is one of the chronic complications of diabetes,and the pathogenesis has not yet been fully clarified. In recent years, more and more studies of CID showed that the changes of NMDA receptor and subunits NR2A and NR2B might be important during the period of the diabetes,and they are associated with the mechanism of cognitive impairment. This article makes a summary on these researches so as to lay the foundation for a further study.

Objective To Analyze CD4+ cell count which embodies curative effect in HIV and patients with AIDS, who have treated with TCM for half a year. Methods According to CD4+cell count, the patients were divided into 4 phases. Their CD4+cell count were analyzed before and after the treatment. Results 1.Rank sun test showed that CD4+cell count were significantly improved in people who used TCM treatment. On the whole, CD4+cell count was(317.76±175.61) in 1 cu mm before treatment, which was(350.60±175.92) in 1 cu mm after treatment, P<0.01. 2. Ridit showed that patients whose CD4+cell count more than 500 were in phase I. Their R=0.614, and the 95%confidence interval was 0.5702 to 0.6579. Patients whose CD4+cell count more than 350 and less than 500 were in phase II. Their R=0.575, and the 95%confidence interval was 0.5439 to 0.6062. Patients whose CD4+cell count more than 200 and less than 350 were inphase III. Their R=0.460 and the 95%confidence interval was 0.4347 to 0.4849. Patients whose CD4+cell count less than 200 were in phase IV. Their R=0.428, and the 95%confidence interval was 0.3971 to 0.4589. There was no overlap between phase III, phase IV and phase I, phase II in 95% confidence interval. Conclusion TCM has the advantages in strengthening vital qi, but that is worse than western medicine in effort of expelling pathogen.

ObjectiveTo explore the relationship between coping modes and depression in stroke patients accepting rehabilitation initially. Methods30 stroke patients were assessed with Medical Coping Modes Questionnaire (MCMQ) and Self-rating Depression Scale (SDS). ResultsThe score of resignation in MCMQ was significantly higher in depression group than in non-depression group （P<0.01). There was correlation between scores of resignation and SDS (r=0.416, P=0.003). ConclusionDepression may relate with resignation in stroke patients admitted in the hospital for rehabilitation initially.