Objective: To investigate the effect of unprotected perineal delivery on perineal side cut rate in primipara during childbirth. Methods: From March 2018 to June 2018, 210 primiparas from the Obstetrics and Gynecology Department of Zhejiang Xiaoshan Hospital were selected in the study.They were divided into observation group (108 cases) and control group (102 cases) according to the delivery method.The control group used protected perineal delivery method, and the observation group used unprotected perineal delivery method.The perineal side cut rate, the perineal integrity rate, the perineal laceration rate were compared between the two groups. Results: 36 cases were treated with perineal side cut in the observation group, accounting for 33.33%, and 48 cases with perineal side cut in the control group, accounting for 47.06%, there was statistically significant difference between the two groups(χ²=4.118, P=0.042).25 patients had complete perineum in the observation group, accounting for 23.15%, and 9 patients in the control group had complete perineum, accounting for 8.82%, there was statistically significant difference between the two groups(χ²=7.933, P=0.005). The observation group had 39 cases of degree Ⅰ perineal laceration (36.11%) and 6 cases of degree Ⅱ perineal laceration (5.56%). The control group had 35 cases of degree Ⅰ perineal laceration (34.31%) and 15 cases of degree Ⅱ perineal laceration (25.49%), there was statistically significant difference between the two groups(χ²=0.074, 4.880, P=0.785, 0.027). Conclusion Unprotected perineal delivery method can reduce the perineal cut rate of primipara and the occurrence of perineal laceration, which is worthy of clinical promotion.

Objective To explore the effect of focus solution mode on the health education for women with non-drug delivery analgesia.Methods A total of 196 cases of natural childbirth women were randomly divided into observation group and control group from March 2013 to February 2016,each with 98 cases. The delivery women in the observation group were given health education with the focus solution mode for non-drug delivery analgesia, and the delivery women in the control group received routine health education for non-drug delivery analgesia. The scores of anxiety and depression, self-care ability and analgesic effect of the two groups were compared before and after interventions.Results At the time of admission, the depression and anxiety scores of the two groups had no statistically significant difference (P>0.05); after interventions, the scores of depression and anxiety for delivery women in the observation group were significantly lower than that of the control group, and the differences were statistically significant (t=3.331, 3.458;P<0.01). After intervention, the maternal self-concept, health knowledge, nursing skills, self responsibility and total score in the observation group were higher than those in the control group, and the differences were statistically significant (t=6.389, 4.254, 3.365, 2.421, 3.541;P<0.05). The number of delivery women with the pain of grade I and below was 102 in the observation group, and the effective analgesia rate was 91.07%; in the control group was 37, and the effective rate of analgesia was 34.69%, so the effective rate of analgesia in the observation group was significantly higher than that of the control group(χ2=70.789,P<0.01).Conclusions Focus solution mode for health education can alleviate the degree of depression and anxiety, improve the self-management ability of delivery women, and improve the effective rate of analgesia.

SARS-CoV-2 infection causes complicated clinical manifestations with variable multi-organ injuries, however, the underlying mechanism, in particular immune responses in different organs, remains elusive. In this study, comprehensive transcriptomic alterations of 14 tissues from rhesus macaque infected with SARS-CoV-2 were analyzed. Compared to normal controls, SARS-CoV-2 infection resulted in dysregulation of genes involving diverse functions in various examined tissues/organs, with drastic transcriptomic changes in cerebral cortex and right ventricle. Intriguingly, cerebral cortex exhibited a hyperinflammatory state evidenced by significant upregulation of inflammation response-related genes. Meanwhile, expressions of coagulation, angiogenesis and fibrosis factors were also up-regulated in cerebral cortex. Based on our findings, neuropilin 1 (NRP1), a receptor of SARS-CoV-2, was significantly elevated in cerebral cortex post infection, accompanied by active immune response releasing inflammatory factors and signal transmission among tissues, which enhanced infection of the central nervous system (CNS) in a positive feedback way, leading to viral encephalitis. Overall, our study depicts a multi-tissue/organ transcriptomic landscapes of rhesus macaque with early infection of SARS-CoV-2, and provides important insights into the mechanistic basis for COVID-19-associated clinical complications.
Animals ; COVID-19/genetics* ; Macaca mulatta ; SARS-CoV-2/genetics* ; Transcriptome