The principal and technique of laser therapy are discoursed upon in chief, current development and application of laser therapy equipment are described by introducing some lasers applied in surgery and medicine such as ophthalmology and photodynamic therapy. Finally, individual view to evolution trend of laser therapy equipment is presented.

Low-level laser biostimulation can be applied in wound therapy to speed up the wound healing,prevent infection and relieve the pain.Although it is still a problem how to select the laser wavelength,power and dose for different wounds because of its unclear mechanism,it has been proved that LLLT has good curative effect and application prospect in wound healing.

Malnutrition is the common complication for the patients with gastrointestinal neoplasms during perioperative period.Tumor and chemotherapy drugs are the main influencing factors of patients with malnutrition.The correct evaluation of the nutritional status in patients is the basis of individualized nutrition therapy.New evaluation methods and nutritional preparations could more help patients with gastrointestinal neoplasms during perioperative period to maintaining good nutritional status.

BACKGROUND: Transmitting law of laser in biological tissues is hard to calculate by mathematical analysis or clinical monitoring. Therefore, numerical simulation is commonly used for its prediction. Monte Carlo simulation is an ideal method for this study. OBJECTIVE: To establish a light dose mathematical model by Monte Carlo method, and to explore the damage of laser to liver tissues.METHODS: Light dose mathematical models of normal tissues and tumor with varied optical parameters were established by Monte Carlo method, and the migration trajectory of ponderosus photons was calculated one by one, and the distribution of photon was analyzed.RESULTS AND CONCLUSION: The decay of photo dose with depth was approximately exponential, and the second peak value will be formed at the verge of the organization. The damage of organization was a process of the threshold value. The dose curved shape was the same as the line of threshold. Using Monte Carlo method, the ransmitting law of laser in tissues of liver tumor was obtained. The results demonstrated that optimal ration of illumination intensity can obtain a shallow but entirely selective depth of tumor necrosis, but to obtain deeper tumor necrosis necessarily means some degree of normal tissue damage.

Repair of peripheral nerve injury is an important topic in the field of neural tissue rehabilitation research.At present there are many new technologies that can realize the treatment of peripheral nerve injury.As the research on the effect of laser radiation on biological mechanism develops,a new method for tissue regeneration is applied to the field of nerve injury repair.This paper is mainly centered on the recovery mechanism of low-level laser radiation,the function recovery after peripheral nerve injury and nerve tissue scarring inhibition.The new progress of laser treatment and nerve injury repair are reviewed.

Laser has been used in the treatment of glaucoma for more than twenty years.Its main applications include laser peripheral iridoplasty,laser trabeculoplasty,laser sclerostomy,and laser cyclophotocoagulation. Other applications such as C02 laser filtering surgery and photodynamic therapy(PDT)have just started.In this paper,a review is provided on the present applications of lasers in the treatment of glaucoma including choice of laser,indications,surgery approach and efficacy.The prospect of the future application is also discussed.

Objective Turn the CT sectional image into three-dimensional imaging based on VTK, in or-der to make better treatment programs for patients with liver cancer. Methods Establish visualization toolkit (VTK) visualization development environment based on VC6.0, obtain patient's CT images in DICOM format, use VTK filter and Laplacian sharpening template for pre-treatment of the images, and then use Ray-Casting algo-rithm for three-dimensional reconstruction. Results We can get three-dimensional images with higher resolu-tion and faster imaging speed through this method, which can also be cut, rotated and zoomed. Conclusion Ray-Casting algorithm based on VTK can be used for the three-dimensional imaging of human liver. With the as-sistance of three-dimensional images, doctors can detect the location of diseased tissue more easily and observe the shapes of diseased tissue intuitively.

Aim To purify a small platide inhibiting platelet aggregation from Agkistrodon Acutus snake venom,and to identify its physical and chemical properties and its effects on the platelet aggregation stimulated by ADP, collagen, and thrombin. Methods Extract snake venom through Superdex 75 gel filtration, ultrafiltration and DEAE-Sepharose CL-6B ionexchange. The purified product was identified by HPLC C_ 18 . The molecular weight was determined by SDS-polyacrylamid gel electrphoresis. Platelet aggragation was measured by nephelometry. Results The molecular weight of the peptide was 7 862 u and its purified from Agkistrodon Acutus snake venom is oelectric point was pH 4.29. This peptide dose-dependently inhibited the platelet aggregation induced by ADP,collagen,and thrombin. Conclusion The method has been proved to be successful for the purification of low molecular weight peptide fraction that inhibits platelet aggregation.

Objective To investigate the relationship between essential hypertension and serum C-reactive protein and plasma lipids.Methods Detecting fasting serum CRP and plasma lipid in each group of hypertensive patients to investigate the relationship between hypertension and fasting serum CRP and plasma lipids.Results CRP,TC,TG,HDL-C and LDL-C level of A,B and C group had significant differences with that of D group(t =4.03, 4.23, 4.61, 3.12, 3.41, 2.76,2.98,2.56,2.64,2.57,3.05,3.68,2.54,3.16,4.28,all P＜0.05).CRP,TC,TG,HDL-C and LDL-C levels between A,B and C group had significant differences(F = 4.68,4.05,3.29,3.59,4.11,all P＜0.05).There was significant correlation between serum CRP level and TC,TG,HDL-C,LDL-C level in 186 hypertension patients(r =0.274,0.356,0.327,0.302,all P＜0.05).Conclusion CRP,TC,TG and LDL-C levels showed significant linear correlation in patients with hypertension.

Objective To identify the changes of mesenteric lymph fluid in cases of uncontrolled hemor-rhagic shock (UHS), which is most often occurred in modem trauma and battle injury. The lymph of gut plays an important role during shock and MODS. The objective of this experiment is to identify the change of mesenteric lymph fluid in UHS. Method Thirty-eight SD rats were canulated with PE 50 catheters separately into right carotid artery,left femoral artery and femoral vein. A serf-made steel catheter was inserted into main mesenteric lymph duct and the mesenteric lymph fluid was collected. The rots were divided into three groups: control group (n=10), controlled hemorrhagic shock (CHS) group (n=10) and UHS group (n=10). After the mean blood pressure was dropped to 40 mmHg by exsanguination, hemorrhage was made by amputation of 75% tail in length and let the stump keep open to bleed. The volume of mesenteric lymph fluid was recorded, and the protein and endotoxin in it were examined, too. Data were analyzed using ANOVA and Chi-square test. Results The mesenteric lymph fluid was decreasing remarkably during shock until it was below 1/4 of normal. After hemorrhage was controlled, the mesentefic lymph fluid increased slowly. However, it kept decreasing persistently in cases of UHS. So did the protein content. The eudotoxin in lymph fluid increased 2 hours after CHS and one hour after UHS. There was significant difference in endotoxin between CHS and UHS 2 hours after shock (P<0.05). Con-dusions The volume and protein content of mesenteric lymph fluid decreased mare significantly in UHS than that in CHS. And contrarily, the endotoxin in lymph fluid increased more significantly in UHS than that in CHS. The change of the mesenteric lymph fluid may be an important cause of MODS after UHS.